D. Chernyshev, V. Klochkova, A. Lelekov, E. Seryak
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引用次数: 0
Abstract
We have described the absorption spectra of acetone extract of the microalga Arthrospira (Spirulina) platensis by mathematical models of carotenoids and chlorophylls in the range from 400 to
700 nm. The obtained values of pigment concentrations agree well with standard methods. A model of the absorption spectrum of acetone extract of A. platensis can be used to determine the concentration of chlorophyll a and carotenoids. The absence of chlorophyll b contribution to the total absorption spectrum was shown. The method of mathematical separation of acetone extract absorption spectrum in Microsoft Excel program was implemented. Calculations performing approximations of pigment concentrations by minimizing the sum of squares of deviations between the true absorption spectrum and its mathematical model have been performed using the spreadsheet processor with the help of the solution search tool.
我们通过类胡萝卜素和叶绿素的数学模型描述了微藻类节旋藻（螺旋藻）丙酮提取物在 400 至 700 纳米波长范围内的吸收光谱。所获得的色素浓度值与标准方法十分吻合。利用丙酮提取物的吸收光谱模型可以确定叶绿素 a 和类胡萝卜素的浓度。研究表明，叶绿素 b 在总吸收光谱中不存在。在 Microsoft Excel 程序中实现了丙酮提取物吸收光谱的数学分离方法。在解决方案搜索工具的帮助下，使用电子表格处理器，通过最小化真实吸收光谱与其数学模型之间偏差的平方和，计算出色素浓度的近似值。
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