Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0648
O. Smirnova, I. Kalashnikova, G. Kurbatov
In this study, we analyzed the absorption spectra of the reaction products of aqueous extracts of mouse tissues with thiobarbituric acid, with the aim to determine the concentration of malonic dialdehyde (MDA) in them. The concentration of MDA is an important part of the analysis of the redox status of tissues, which is important in the study of inflammatory reactions, for example, after various stressful effects, as well as in the study of aging. In normal practice, they calculate the concentration of MDA in a solution by its optical density at 532 nm, then these data are related with similar solutions of the tetramethoxypropane (TMP) reaction with known concentration. We have shown that in cases of submicromolar MDA content, fluctuations in the nonspecific background level of the optical density of solutions can be commensurate to the magnitude of the actual absorption signal of the resulting colored adduct. Solutions of biological tissue extracts, due to the additional content of proteins, lipids and carbohydrates, are characterized by turbidity, which distorts the absorption spectrum non-linearly. The second derivatives of the absorption spectra deprived of background scattering distortions and can be used for automatic software calculation of the pigment content. Obtaining correct derivatives is complicated by the need to smooth the original spectra. We used two smoothing methods: the moving average method and the Savitsky–Goley filter with a polynomial of the third degree. We compared the data obtained on the basis of measuring the optical densities of solutions at 532 nm with those based on the analysis of the second derivatives of their absorption spectra, and also on the basis of integral sums of the second derivatives in the range of 520-550 nm. The results of calculations using the second derivatives gave 2-5 times lower concentrations of MDA than those obtained from optical densities at the maximum absorption of the adduct. At the same time, the convergence of the data, especially when using integral sums of the second derivatives, turned out to be significantly better than for the zero order, and the resulting errors were 2-3 times smaller.
{"title":"MDA PHOTOMETRY IN SOLUTIONS: COMPARISON OF METHODS OF DIRECT OPTICAL DENSITY DATA AND DERIVATIVE SPECTROSCOPY","authors":"O. Smirnova, I. Kalashnikova, G. Kurbatov","doi":"10.29039/rusjbpc.2023.0648","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0648","url":null,"abstract":"In this study, we analyzed the absorption spectra of the reaction products of aqueous extracts of mouse tissues with thiobarbituric acid, with the aim to determine the concentration of malonic dialdehyde (MDA) in them. The concentration of MDA is an important part of the analysis of the redox status of tissues, which is important in the study of inflammatory reactions, for example, after various stressful effects, as well as in the study of aging. In normal practice, they calculate the concentration of MDA in a solution by its optical density at 532 nm, then these data are related with similar solutions of the tetramethoxypropane (TMP) reaction with known concentration. We have shown that in cases of submicromolar MDA content, fluctuations in the nonspecific background level of the optical density of solutions can be commensurate to the magnitude of the actual absorption signal of the resulting colored adduct. Solutions of biological tissue extracts, due to the additional content of proteins, lipids and carbohydrates, are characterized by turbidity, which distorts the absorption spectrum non-linearly. The second derivatives of the absorption spectra deprived of background scattering distortions and can be used for automatic software calculation of the pigment content. Obtaining correct derivatives is complicated by the need to smooth the original spectra. We used two smoothing methods: the moving average method and the Savitsky–Goley filter with a polynomial of the third degree. We compared the data obtained on the basis of measuring the optical densities of solutions at 532 nm with those based on the analysis of the second derivatives of their absorption spectra, and also on the basis of integral sums of the second derivatives in the range of 520-550 nm. The results of calculations using the second derivatives gave 2-5 times lower concentrations of MDA than those obtained from optical densities at the maximum absorption of the adduct. At the same time, the convergence of the data, especially when using integral sums of the second derivatives, turned out to be significantly better than for the zero order, and the resulting errors were 2-3 times smaller.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"113 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141377671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0635
I. Golovchenko, V. Ratnikov
This paper provides a means of analysing a four-component system using a model consistent with the theory of interceptor-protector action. The given model is a further development of already well-studied three-component systems and was previously demonstrated without a symbolic and graphical solution. Elements of the theory present a complete analytical algorithm that can potentially be applied to any system of DNA-specific mixtures of aromatic drugs; components that inhibit the deleterious effects of noxious agents that interfere with biologically relevant molecular interactions, or conversely, inhibitors of catalytic agents. Such analytical models are important in the context of designing new drugs to regulate their efficacy and targeting. For the purposes of this article, the fourth component of the system is considered to be specific to the interceptor/protector in a three-component system, acting as a regulator of the inhibitory activity of the third component, with any interaction with the other two components being completely ignored. A full graphical representation of the model is given only for the extreme cases where the affinity to the fourth component is either completely absent or infinite. The qualitative analysis of the graph of the real four-component system is carried out by tracking the proximity of the experimental points to one of the previously mentioned graphs.
本文提供了一种使用与拦截器-保护器作用理论一致的模型分析四组件系统的方法。所给出的模型是对已经研究得很透彻的三组份系统的进一步发展，之前已经在没有符号和图形解决方案的情况下进行了演示。该理论的各要素提出了一种完整的分析算法，有可能应用于任何 DNA 特异性芳香药物混合物系统；抑制干扰生物相关分子相互作用的有害制剂的有害效应的成分，或反过来说，抑制催化制剂的成分。此类分析模型对于设计新药物以调节其药效和靶向性非常重要。在本文中，系统中的第四组分被认为是三组分系统中的特异性拦截剂/保护剂，对第三组分的抑制活性起调节作用，而与其他两组分的任何相互作用则被完全忽略。只有在与第四种成分的亲和力完全不存在或无限大的极端情况下，才会给出模型的完整图示。通过跟踪实验点与前面提到的图形之一的接近程度，可以对真实的四成分系统图形进行定性分析。
{"title":"ANALYSIS OF THE FOUR-COMPONENT SYSTEM REDUCED MODEL CONSIDERING THE THEORY OF INTERCEPTOR-PROTECTOR ACTION","authors":"I. Golovchenko, V. Ratnikov","doi":"10.29039/rusjbpc.2023.0635","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0635","url":null,"abstract":"This paper provides a means of analysing a four-component system using a model consistent with the theory of interceptor-protector action. The given model is a further development of already well-studied three-component systems and was previously demonstrated without a symbolic and graphical solution. Elements of the theory present a complete analytical algorithm that can potentially be applied to any system of DNA-specific mixtures of aromatic drugs; components that inhibit the deleterious effects of noxious agents that interfere with biologically relevant molecular interactions, or conversely, inhibitors of catalytic agents. Such analytical models are important in the context of designing new drugs to regulate their efficacy and targeting. For the purposes of this article, the fourth component of the system is considered to be specific to the interceptor/protector in a three-component system, acting as a regulator of the inhibitory activity of the third component, with any interaction with the other two components being completely ignored. A full graphical representation of the model is given only for the extreme cases where the affinity to the fourth component is either completely absent or infinite. The qualitative analysis of the graph of the real four-component system is carried out by tracking the proximity of the experimental points to one of the previously mentioned graphs.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"4 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141379617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0636
V. Klochkova, D. Shumeiko, A. Lelekov
The study of the size structure of cultures of marine red Purphurium purpurium (Vogu) Ross and green Tetraselmis viridis Rouch microalgae under batch and quasi-continuous modes of cultivation with different irradiance was carried out. Cell size distribution was determined using a laser analyzer "Laska-TM" and a microscope with a camera. Based on a sample of 50 random cells, the mean diameter value was found. It is shown that in the accumulative mode of cultivation the cell size remains constant. The average cell diameter of Porphyridium cells ranged from 8.9 to 9.3 μm, and that of Tetraselmis from 6.8 to 7.4 μm. The result obtained is supported by literature data on cultivation of the microalga Chlorella protothecoides. The quasi-continuous regime was carried out in the exponential growth phase, when the culture density is low and the only limiting factor is the light flux intensity. It was experimentally found that the average cell diameter of both microalgae increased with increasing irradiance. It is shown that the values of average diameters obtained on the laser analyzer are overestimated than the results obtained using a microscope. The obtained data can serve as a basis for the development of growth models of the accumulative culture of microalgae. The constancy of the average cell size makes it possible to abandon complex distributed cell population models.
{"title":"SIZE STRUCTURE OF PORPHYRIDIUM PURPUREUM AND TETRASELMIS VIRIDIS POPULATIONS UNDER DIFFERENT CULTIVATION REGIMES","authors":"V. Klochkova, D. Shumeiko, A. Lelekov","doi":"10.29039/rusjbpc.2023.0636","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0636","url":null,"abstract":"The study of the size structure of cultures of marine red Purphurium purpurium (Vogu) Ross and green Tetraselmis viridis Rouch microalgae under batch and quasi-continuous modes of cultivation with different irradiance was carried out. Cell size distribution was determined using a laser analyzer \"Laska-TM\" and a microscope with a camera. Based on a sample of 50 random cells, the mean diameter value was found. It is shown that in the accumulative mode of cultivation the cell size remains constant. The average cell diameter of Porphyridium cells ranged from 8.9 to 9.3 μm, and that of Tetraselmis from 6.8 to 7.4 μm. The result obtained is supported by literature data on cultivation of the microalga Chlorella protothecoides. The quasi-continuous regime was carried out in the exponential growth phase, when the culture density is low and the only limiting factor is the light flux intensity. It was experimentally found that the average cell diameter of both microalgae increased with increasing irradiance. It is shown that the values of average diameters obtained on the laser analyzer are overestimated than the results obtained using a microscope. The obtained data can serve as a basis for the development of growth models of the accumulative culture of microalgae. The constancy of the average cell size makes it possible to abandon complex distributed cell population models.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"33 S117","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141377743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0653
I. Parfyonova, A. Soldatov
Information on critical and lethal oxygen concentrations for some Black Sea fish is summarized. It is shown that the Black Sea fish species can be conditionally divided into two groups with respect to resistance to oxygen deficiency. Group 1 with high sensitivity and low resistance to hypoxia, which is represented mainly by pelagic and some bottom-dwelling fish species (Trachurus mediterraneus, Spicara smaris, Merlangius merlangus). Group 2 with low sensitivity and high resistance to hypoxia, representatives of which are mainly bottom and a number of bottom fish species (Scorpaena porcus, Mullus barbatus barbatus, Uranoscopus scaber). Among these species, the most resistant and least sensitive to hypoxia species are the S. porcus and the Neogobius melanostomus, which are widely represented along the Black Sea coast. The lowest values of critical and lethal oxygen tensions were obtained for them: 33-36 and 4-11 kPa, respectively. The range of dependent respiration is 22-31 kPa.
{"title":"CRITICAL AND LETHAL OXYGEN CONCENTRATIONS FOR SOME BLACK SEA FISH (SHORT REVIEW)","authors":"I. Parfyonova, A. Soldatov","doi":"10.29039/rusjbpc.2023.0653","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0653","url":null,"abstract":"Information on critical and lethal oxygen concentrations for some Black Sea fish is summarized. It is shown that the Black Sea fish species can be conditionally divided into two groups with respect to resistance to oxygen deficiency. Group 1 with high sensitivity and low resistance to hypoxia, which is represented mainly by pelagic and some bottom-dwelling fish species (Trachurus mediterraneus, Spicara smaris, Merlangius merlangus). Group 2 with low sensitivity and high resistance to hypoxia, representatives of which are mainly bottom and a number of bottom fish species (Scorpaena porcus, Mullus barbatus barbatus, Uranoscopus scaber). Among these species, the most resistant and least sensitive to hypoxia species are the S. porcus and the Neogobius melanostomus, which are widely represented along the Black Sea coast. The lowest values of critical and lethal oxygen tensions were obtained for them: 33-36 and 4-11 kPa, respectively. The range of dependent respiration is 22-31 kPa.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"163 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141375916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0644
A. Stepanov, A. Popov, A. Kovalenko, D. Yumanov, A. Dimitrieva, K. Sergeeva, D. Dementiev, A. Nikolskaya, V. Tulovchikov, D. Tetelbaum
This paper presents a molecular dynamics simulation of hypersonic wave propagation in liquid–solid systems such as aqueous solutions of NaCl and glass (containing Na ions); the role of waveguide properties of Na+–(H2O)n–type clusters is analyzed; the role of Na+–(H2O)n-type clusters in the propagation of hypersonic waves is discussed. The interaction between hypersound and cellular organelles was studied numerically and compared with biological experiments on seeds of spring soft wheat variety Ekada 70, in particular on the effect of this effect on their growth. hypersonic irradiation affects the biometric parameters of spring soft wheat, seed weight and yield. In this case, the time of exposure to this irradiation is of great importance. The smallest plant height in the variant with seed irradiation for 30 minutes was 71 s, and the productive bushiness was 1.8. The indices of general bushiness and the average number of normally developed stems producing grain per plant were also the best in the variant with exposure to seed material irradiation for 100 seconds, the worst in the variant of 30 minutes. From this we can conclude that hypersonic irradiation affects the biometric parameters of spring soft wheat, seed weight and yield. In this case, the time of exposure to this irradiation is of great importance. The data obtained as a result of modeling show that the water-glass interface is a channel for energy transfer from a source of hypersonic waves to a biological object.
{"title":"GENERATION OF HYPERSONIC WAVES UPON IRRADIATION OF SILICON WITH LIGHT, THEIR PROPAGATION AND INFLUENCE ON LIVING ORGANISMS: COMPUTER SIMULATION AND EXPERIMENT","authors":"A. Stepanov, A. Popov, A. Kovalenko, D. Yumanov, A. Dimitrieva, K. Sergeeva, D. Dementiev, A. Nikolskaya, V. Tulovchikov, D. Tetelbaum","doi":"10.29039/rusjbpc.2023.0644","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0644","url":null,"abstract":"This paper presents a molecular dynamics simulation of hypersonic wave propagation in liquid–solid systems such as aqueous solutions of NaCl and glass (containing Na ions); the role of waveguide properties of Na+–(H2O)n–type clusters is analyzed; the role of Na+–(H2O)n-type clusters in the propagation of hypersonic waves is discussed. The interaction between hypersound and cellular organelles was studied numerically and compared with biological experiments on seeds of spring soft wheat variety Ekada 70, in particular on the effect of this effect on their growth. hypersonic irradiation affects the biometric parameters of spring soft wheat, seed weight and yield. In this case, the time of exposure to this irradiation is of great importance. The smallest plant height in the variant with seed irradiation for \u000030 minutes was 71 s, and the productive bushiness was 1.8. The indices of general bushiness and the average number of normally developed stems producing grain per plant were also the best in the variant with exposure to seed material irradiation for 100 seconds, the worst in the variant of 30 minutes. From this we can conclude that hypersonic irradiation affects the biometric parameters of spring soft wheat, seed weight and yield. In this case, the time of exposure to this irradiation is of great importance. The data obtained as a result of modeling show that the water-glass interface is a channel for energy transfer from a source of hypersonic waves to a biological object.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"98 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141377151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0645
B. Zenteno Mateo, M. Morales, R. Serrano, A. Cervantes Tavera, R. Jose Israel, Adrian Ernandes Sant'yago
In this research work proposal, a way to generalize the thermokinetic Oregonator model in order to simulate the dynamics of oscillating chemical reactions. The simulations to be carried out use elementary numerical methods using the Runge Kutta method, which are a very good approximation of the mathematical solutions of the models of these complex systems of nonlinear chemistry. The implications of the results of the simulations lie in the reproduction of the nonlinear dynamics of thermokinetic oregonator model to temperatures different from room temperature. Additionally, the linear stability analysis is established to find the oscillation regions in the parameter space of the proposed model.
{"title":"GENERALIZATION OF THE THERMOKINETIC OREGONATOR MODEL","authors":"B. Zenteno Mateo, M. Morales, R. Serrano, A. Cervantes Tavera, R. Jose Israel, Adrian Ernandes Sant'yago","doi":"10.29039/rusjbpc.2023.0645","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0645","url":null,"abstract":"In this research work proposal, a way to generalize the thermokinetic Oregonator model in order to simulate the dynamics of oscillating chemical reactions. The simulations to be carried out use elementary numerical methods using the Runge Kutta method, which are a very good approximation of the mathematical solutions of the models of these complex systems of nonlinear chemistry. The implications of the results of the simulations lie in the reproduction of the nonlinear dynamics of thermokinetic oregonator model to temperatures different from room temperature. Additionally, the linear stability analysis is established to find the oscillation regions in the parameter space of the proposed model.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"57 s198","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141376498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0638
A. Brilkov, E. Brilkova, I. Jabrun, V. Ganusov, Yu. Loginov, A. Shuvaev
In the present work, a mathematical model for the distribution of transgenic bacteria cells by the number of plasmids contained in them is constructed. This makes it possible to study the patterns of survival of transgenic microorganisms under certain environmental conditions, taking into account various characteristics of transgenic strains, for example, the stability of plasmids in new cells, the “population cost” of maintaining conjugative and non-conjugative plasmids, the efficiency of expression of genes cloned on plasmids, and others. The "plasmid paradox" lies in the fact that although the presence of plasmids containing cloned genes usually reduces the specific growth rate of transgenic bacteria, but with prolonged growth of bacteria under non-selective conditions ("experimental evolution", 20-30 or more generations of the original form without antibiotics) “cost of fitness” is reduced by an order of magnitude or two. An analysis of the experimental results leads to the conclusion that this is due to a change in the difference in the growth rates of plasmid and plasmid-free cells, with a change in the probability of formation of plasmid-free cells, the copy number of plasmids, and the expression of cloned genes in cells of transgenic bacteria under various environmental conditions.
{"title":"MATHEMATICAL MODELING OF THE EXPERIMENTAL EVOLUTION OF TRANSGENIC BACTERIA: “PLASMID PARADOX”","authors":"A. Brilkov, E. Brilkova, I. Jabrun, V. Ganusov, Yu. Loginov, A. Shuvaev","doi":"10.29039/rusjbpc.2023.0638","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0638","url":null,"abstract":"In the present work, a mathematical model for the distribution of transgenic bacteria cells by the number of plasmids contained in them is constructed. This makes it possible to study the patterns of survival of transgenic microorganisms under certain environmental conditions, taking into account various characteristics of transgenic strains, for example, the stability of plasmids in new cells, the “population cost” of maintaining conjugative and non-conjugative plasmids, the efficiency of expression of genes cloned on plasmids, and others. The \"plasmid paradox\" lies in the fact that although the presence of plasmids containing cloned genes usually reduces the specific growth rate of transgenic bacteria, but with prolonged growth of bacteria under non-selective conditions (\"experimental evolution\", 20-30 or more generations of the original form without antibiotics) “cost of fitness” is reduced by an order of magnitude or two. An analysis of the experimental results leads to the conclusion that this is due to a change in the difference in the growth rates of plasmid and plasmid-free cells, with a change in the probability of formation of plasmid-free cells, the copy number of plasmids, and the expression of cloned genes in cells of transgenic bacteria under various environmental conditions.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"25 36","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141379819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0649
A. Botin, D. Mashal, T. Popova, M.G.H. Rizk, A. Cordova
The paper considers one of the most important directions in modern pharmacology - targeted delivery of medicines, namely the directed transport of medicinal substance to given area of body, which is realized with help of carriers, which, as a rule, have sizes of tens or hundreds of nanometers, different nature and chemical structure. The delivery of antitumor drugs using nanoparticles is being discussed. Immobilization of drugs on nanocarriers makes it possible to increase their bioavailability. Various graphene derivatives - graphene oxide (GO) and reduced graphene oxide (RGO) - are being tested as carriers for drug delivery. There are several approaches for targeted drug delivery in oncology. The first, simple one is the attachment of both low– and high-molecular preparation to the surface of the carrier directly. The drug doxorubicin is firmly bound to surface of graphene oxide and is released only in acidic environment of tumor. The second, more complex method is to attach to surface of carrier not only active substance, but also guiding molecules - ligands. Sometimes ligand itself can be a drug at the same time. Polygraphene (PG) is an original modified analogue of thermally split graphite, obtained in the form of new form of expanded graphite, after repeated chemical modification and thermal activation, it is reduced to the characteristics of a layered material with stacks of carbon monolayers of smaller multiplicity (from 5 to 50), up to single sheets of graphene. The results of tests of PG as an effective basis for the immobilization of enzymes are presented, in particular, on the example of antitumor enzyme L-lysine-α-oxidase. These data indicate prospects of possible biomedical use of PG in oncology, namely, in treatment of intestinal cancer. Modified forms of graphene and polygraphene should be considered as new carrier of drugs.
{"title":"CARBON NANOCOMPOSITES IN MEDICINE: GRAPHENE AND POLYGRAPHENE AS POSSIBLE DRUG DELIVERY VEHICLE FOR INTESTINAL ONCOLOGY","authors":"A. Botin, D. Mashal, T. Popova, M.G.H. Rizk, A. Cordova","doi":"10.29039/rusjbpc.2023.0649","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0649","url":null,"abstract":"The paper considers one of the most important directions in modern pharmacology - targeted delivery of medicines, namely the directed transport of medicinal substance to given area of body, which is realized with help of carriers, which, as a rule, have sizes of tens or hundreds of nanometers, different nature and chemical structure. The delivery of antitumor drugs using nanoparticles is being discussed. Immobilization of drugs on nanocarriers makes it possible to increase their bioavailability. Various graphene derivatives - graphene oxide (GO) and reduced graphene oxide (RGO) - are being tested as carriers for drug delivery. There are several approaches for targeted drug delivery in oncology. The first, simple one is the attachment of both low– and high-molecular preparation to the surface of the carrier directly. The drug doxorubicin is firmly bound to surface of graphene oxide and is released only in acidic environment of tumor. The second, more complex method is to attach to surface of carrier not only active substance, but also guiding molecules - ligands. Sometimes ligand itself can be a drug at the same time. Polygraphene (PG) is an original modified analogue of thermally split graphite, obtained in the form of new form of expanded graphite, after repeated chemical modification and thermal activation, it is reduced to the characteristics of a layered material with stacks of carbon monolayers of smaller multiplicity (from 5 to 50), up to single sheets of graphene. The results of tests of PG as an effective basis for the immobilization of enzymes are presented, in particular, on the example of antitumor enzyme L-lysine-α-oxidase. These data indicate prospects of possible biomedical use of PG in oncology, namely, in treatment of intestinal cancer. Modified forms of graphene and polygraphene should be considered as new carrier of drugs.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"28 S102","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141376706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0642
V. Bystrov, S. Filippov, I. Likhachev, O. Ledeneva, E. Belova
The paper presents calculations of the dipole, polar, and electronic properties of helical nanostructures based on dileucine (LL) of different chirality (L, D) using the AM1, PM3 methods from the HyperChem software package. It has been shown that the physical properties of dileucine-based nanotubes are close to those of diphenylalanine nanotubes (FF PNT). For nanostructures based on dileucine of left chirality L-LL, the existing experimental data make it possible to isolate from crystallographic structures molecular nanostructures of helical nanotubes that have right chirality D, which corresponds to the law of chirality change upon transition to the next level of self-organization. Model helical structures of nanotubes based on dileucine of right chirality D-LL were also constructed, for which there are no experimental data. Calculations of chirality by the method of mixed vector-scalar product of dipole moments of dipeptides showed that the law of changing the type of chirality is also valid for model helical nanotubes based on the D-LL. These results can be the basis for the synthesis of new experimental nanostructures based on right-handed dipeptides.
{"title":"SIMULATION AND COMPUTER STUDY OF THE CHIRAL PROPERTIES OF PEPTIDE NANOTUBES BASED ON DILEUCINE","authors":"V. Bystrov, S. Filippov, I. Likhachev, O. Ledeneva, E. Belova","doi":"10.29039/rusjbpc.2023.0642","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0642","url":null,"abstract":"The paper presents calculations of the dipole, polar, and electronic properties of helical nanostructures based on dileucine (LL) of different chirality (L, D) using the AM1, PM3 methods from the HyperChem software package. It has been shown that the physical properties of dileucine-based nanotubes are close to those of diphenylalanine nanotubes (FF PNT). For nanostructures based on dileucine of left chirality L-LL, the existing experimental data make it possible to isolate from crystallographic structures molecular nanostructures of helical nanotubes that have right chirality D, which corresponds to the law of chirality change upon transition to the next level of self-organization. Model helical structures of nanotubes based on dileucine of right chirality D-LL were also constructed, for which there are no experimental data. Calculations of chirality by the method of mixed vector-scalar product of dipole moments of dipeptides showed that the law of changing the type of chirality is also valid for model helical nanotubes based on the D-LL. These results can be the basis for the synthesis of new experimental nanostructures based on right-handed dipeptides.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"42 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141377878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.29039/rusjbpc.2023.0650
A. Botin, M.G.H. Rizk, T. Popova, A. Cordova
The article considers one of the most important factors allowing to determine the possibility of wide and safe use of graphene nanoobjects in modern biomedicine - this is the biocompatibility factor, namely, the interaction of a graphene-containing substance with a given part of the body, which is realized at different scales and at different levels of organization of living matter. Graphene and its derivatives have shown exceptional properties and potential for various applications. While graphene derivatives as graphene-oxide (GO), reduced graphene-oxide (rGO), few-layers graphene (FLG), and multi-layers graphene (MLG) exhibit similar properties to graphene, more research is needed to address scalability and cost-effectiveness for practical applications. In tissue engineering, graphene-based materials have shown promise in scaffolds, biosensors, and drug delivery systems, but optimizing biocompatibility and functionalization strategies are crucial for safe and effective use. This work is a try to better understanding the complex interactions between graphene and biological systems, including cells, tissues, and organs, which is necessary for future research and expanding the use of graphene in biomedical applications.
{"title":"SAFETY OF CARBON NANOCARRIERS IN BIOMEDICAL APPLICATIONS: BIOCOMPATIBILITY OF GRAPHENE AND ITS DERIVATIVES","authors":"A. Botin, M.G.H. Rizk, T. Popova, A. Cordova","doi":"10.29039/rusjbpc.2023.0650","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0650","url":null,"abstract":"The article considers one of the most important factors allowing to determine the possibility of wide and safe use of graphene nanoobjects in modern biomedicine - this is the biocompatibility factor, namely, the interaction of a graphene-containing substance with a given part of the body, which is realized at different scales and at different levels of organization of living matter. Graphene and its derivatives have shown exceptional properties and potential for various applications. While graphene derivatives as graphene-oxide (GO), reduced graphene-oxide (rGO), few-layers graphene (FLG), and multi-layers graphene (MLG) exhibit similar properties to graphene, more research is needed to address scalability and cost-effectiveness for practical applications. In tissue engineering, graphene-based materials have shown promise in scaffolds, biosensors, and drug delivery systems, but optimizing biocompatibility and functionalization strategies are crucial for safe and effective use. This work is a try to better understanding the complex interactions between graphene and biological systems, including cells, tissues, and organs, which is necessary for future research and expanding the use of graphene in biomedical applications.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"36 1‐2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141381087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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