INVESTIGATION OF THE POSSIBILITY OF MEASURING GLYCATED HEMOGLOBIN BY SCANNING FLOW CYTOMETRY

Abstract

The article explores the possibility of measuring glycated hemoglobin in single erythrocytes using scanning flow cytometry. Calculations of the intensity of scattered radiation from an erythrocyte in a wide angular range (light scattering indicatrix) were carried out for wavelengths at the maximum of the absorption band of glycated hemoglobin. The maximum sensitivity for the concentration of glycated hemoglobin was set at a wavelength of 415 nm. As a result, it is possible to reliably separate the concentrations of glycated hemoglobin in donors and patients with diagnosed diabetes with a difference in HbA1c concentrations of more than 5% on the existing practical implementation of a scanning flow cytometer, which includes lasers: 405 nm (30 mW, Radius, Coherent Inc., Santa Clara, USA) and 660 nm (LM–660–20–S, 40 mW). A theoretical calculation for a wavelength of 415 nm showed that a noticeable difference in the value of the scattering intensity (more than 10%) is observed only in the case of a difference in the concentrations of glycated hemoglobin of at least 3%. Thus, if a laser with a wavelength of 415 nm is installed on the existing practical implementation of the device, then the determination of glycated hemoglobin will become possible with an accuracy of no more than 3%.