{"title":"Nanobody immobilization on magnetic nanoparticles via monomeric streptavidin-biotin specific interaction for aflatoxin adsorption","authors":"Hongpeng Wang, Alexey Tarabarov, Qingqing Rao, Xing Wang, Yiyu Qi, Yongqi Wang, Zhuqian Xiao, Changjiang Lv, Jiayao Yang, Jun Huang, Shengxiang Yang","doi":"10.1007/s42114-024-00893-8","DOIUrl":null,"url":null,"abstract":"<div><p>A new magnetic aflatoxin (AF) adsorbent was synthesized by nanobody (Nb28) immobilization on magnetic nanoparticles (MNPs). Monomeric streptavidin (mSA)-biotin specific interaction technology was used as a linker for nanobody immobilization. As a magnetic solid support, Fe<sub>3</sub>O<sub>4</sub> nanoparticles were modified with tetraethyl orthosilicate (TEOS), (3-Aminopropyl) trimethoxysilane (APTMS), and glutaraldehyde (GA). According to the characterization results, modified MNPs were monodisperse, paramagnetic, with average diameter of 450 nm and they showed less susceptibility to oxidation on air. MNPs-Nb28 and MNPs-mSA-Nb28 enzymatic activity was analyzed by high-performance liquid chromatography (HPLC) and showed the highest level of adsorption under optimal conditions for aflatoxin G<sub>2</sub> (AFG<sub>2</sub>), reaching concentration reduction up to 96.9% and 97.1%, respectively. MNPs-Nb28 showed higher stability over storage time, temperature, and pH compare to MNPs-mSA-Nb28. The maximum AF adsorption capacities of MNPs-Nb28 and MNPs-mSA-Nb28 were 193 µg·g<sup>−1</sup> and 194 µg·g<sup>−1</sup>, respectively. Both nanocomposites were proven to be a reliable tool for fast and efficient removal of different aflatoxins (AFs) from solution.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7220,"journal":{"name":"Advanced Composites and Hybrid Materials","volume":null,"pages":null},"PeriodicalIF":23.2000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Composites and Hybrid Materials","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s42114-024-00893-8","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, COMPOSITES","Score":null,"Total":0}
引用次数: 0
Abstract
A new magnetic aflatoxin (AF) adsorbent was synthesized by nanobody (Nb28) immobilization on magnetic nanoparticles (MNPs). Monomeric streptavidin (mSA)-biotin specific interaction technology was used as a linker for nanobody immobilization. As a magnetic solid support, Fe3O4 nanoparticles were modified with tetraethyl orthosilicate (TEOS), (3-Aminopropyl) trimethoxysilane (APTMS), and glutaraldehyde (GA). According to the characterization results, modified MNPs were monodisperse, paramagnetic, with average diameter of 450 nm and they showed less susceptibility to oxidation on air. MNPs-Nb28 and MNPs-mSA-Nb28 enzymatic activity was analyzed by high-performance liquid chromatography (HPLC) and showed the highest level of adsorption under optimal conditions for aflatoxin G2 (AFG2), reaching concentration reduction up to 96.9% and 97.1%, respectively. MNPs-Nb28 showed higher stability over storage time, temperature, and pH compare to MNPs-mSA-Nb28. The maximum AF adsorption capacities of MNPs-Nb28 and MNPs-mSA-Nb28 were 193 µg·g−1 and 194 µg·g−1, respectively. Both nanocomposites were proven to be a reliable tool for fast and efficient removal of different aflatoxins (AFs) from solution.
期刊介绍：
Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field.
The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest.
Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials.
Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.
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