Hepatoprotective Effect of Aqueous Neem Leaves Extract on Hepatocytes and Nuclear Diameter of Albino Wistar Rats Induced with Erythromycin Toxicity

Life and Science Pub Date : 2024-05-05 DOI:10.37185/lns.1.1.572

N. Jamshed, Iffat Raza, Mehwish Razzaq, Naureen Jamshed, Aaqiba Rasheed, Lubna Faisal

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Abstract

Objective: To evaluate the hepatoprotective effects of Neem leaves extract on hepatocyte and nucleardiameters of Albino Wistar rats induced by erythromycin hepatotoxicity.Study Design: Laboratory-based Experimental study.Place and Duration of Study: The study was carried out at the Department of Anatomy and Animal House ofBaqai Medical University (BMU) in Karachi, Pakistan from January 2019 to March 2019.Methods: A total of 80 Albino Wistar rats were divided equally into 4 groups. The control group was markedas Group A with no interventions. In Group B only erythromycin was administered. For Group C botherythromycin and aqueous Neem leave Extract were given and for Group D only aqueous neem leave wasgiven. Animals were sacrificed and the liver weight of Albino Wistar rats was measured.Results: It was observed that rats of experimental control group A noticed no significant change inhepatocytes13.19± 0.44μm and nuclear diameters 7.18±0. 28μm (Figure.1) as this group had no interventionreceived. In group B hepatocyte diameter markedly increased by 16.54±0.6μm and a decrease in nucleardiameter of 5.20±0.30μm (p<0.01) with loss of architecture, ballooning, and edematous changes (Figure.2, 3and 4) was observed as this group received only erythromycin while group C received both erythromycin andaqueous Neem leaf extract showed histological architecture disrupted to some extent and hepatocytes14.49±80μm and nuclear diameter 6.13±0.17μm were started to improve its normal sizes (Figure.5). Group Dobserved no significant changes in hepatocytes 13.0±0.52μm and nuclear diameters 7.14±0.30μm as thisgroup only received aqueous neem leave extract (Figure.6).Conclusion: According to the current study, Erythromycin exerts oxidative stress and Changes in hepatocytesand nuclear diameters were noticeably improved by aqueous neem leaf extract, which is a powerfulantioxidant. How to cite this: Jamshed N, Raza I, Razzaq M, Jamshed N, Rasheed A, Faisal L. Hepatoprotective Effect of Aqueous Neem Leaves Extract on Hepatocytes and Nuclear Diameter of Albino Wistar Rats Induced with Erythromycin Toxicity. Life and Science. 2024; 5(2): 228-236. doi: http://doi.org/10.37185/LnS.1.1.572

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苦楝树叶水提取物对红霉素毒性诱导的白化 Wistar 大鼠肝细胞和核直径的保肝作用

研究目的评估印楝叶提取物对红霉素肝毒性诱导的白化Wistar大鼠肝细胞和核心功能的保护作用：研究地点和时间：研究于2019年1月至2019年3月在巴基斯坦卡拉奇巴凯医科大学（BMU）解剖学系和动物房进行：将 80 只白化 Wistar 大鼠平均分为 4 组。对照组为 A 组，不采取任何干预措施。B 组只使用红霉素。C 组给予红霉素和苦楝水提取物，D 组仅给予苦楝水提取物。动物被处死，并测量白化 Wistar 大鼠的肝脏重量：结果：实验对照 A 组大鼠的肝细胞直径为 13.19±0.44μm，核直径为 7.18±0.28μm（图 1）。B 组肝细胞直径明显增大 16.54±0.6μm，核直径减小 5.20±0.30μm（p<0.01），并伴有结构丧失、气球和水肿变化（图 2、C组同时接受红霉素和水相印楝叶提取物治疗，组织学结构受到一定程度的破坏，肝细胞直径（14.49±80μm）和核直径（6.13±0.17μm）开始恢复正常大小（图 5）。D组由于只接受楝树叶水提取物，肝细胞13.0±0.52μm和核直径7.14±0.30μm无明显变化（图6）：根据目前的研究，印楝叶水提取物是一种强有力的抗氧化剂，它能明显改善红霉素对肝细胞的氧化应激和肝细胞核直径的变化。如何引用Jamshed N, Raza I, Razzaq M, Jamshed N, Rasheed A, Faisal L. Hepatoprotective Effect of Aqueous Neem Leaves Extract on Hepatocytes and Nuclear Diameter of Albino Wistar Rats Induced with Erythromycin Toxicity.生命与科学2024; 5(2):228-236. DOI: http://doi.org/10.37185/LnS.1.1.572

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