Effects of Alpha Lipoic Acid Supplementation on Slow Skeletal Muscle Mass and Contractile Functions in Type 2 Diabetic Male Sprague Dawley Rats

Life and Science Pub Date : 2024-05-05 DOI:10.37185/lns.1.1.408

Barkat Ullah Khan, Iftekhar Yousef, Sidra Arshad, Faiza Ikram, Amjad Ali, Ikram Ullah

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Abstract

Objective: To see the Effects of Alpha-Lipoic Acid (ALA) supplementation on slow skeletal muscle mass andcontractile functions in type 2 diabetic male Sprague Dawley rats.Study Design: Quasi-experimental study.Place and Duration of Study: The study was carried out at the Physiology Research Lab, Army MedicalCollege, Rawalpindi, Pakistan from June 2019 to April 2021 in collaboration with the National Institute of Health(NIH) Islamabad, Pakistan.Methods: Sixty adult SD rats were divided into three equal groups. Rats were fed on a standard diet as per NIHprotocols. After 2 weeks type 2 Diabetes mellitus (T2DM) was induced in groups 2 and 3 by injecting low dose35mg of streptozotocin (STZ) in the abdomen intraperitoneally. T2DM was successfully developed andconfirmed by measuring glucose levels through a glucometer. Group 3 was injected with Alpha Lipoic acid30mg/kg/day at the lower abdomen for the next two weeks. After 04 weeks, soleus muscles were dissected.The animal data acquisition unit (iWorx) was used for assessing the contractile functions of soleus.Results: Alpha Lipoic acid group showed improvement in muscle mass, muscle tension strength, and recoveryfrom fatigue after applying fatigue protocol as compared to group 2.Conclusion: Alpha Lipoic acid supplementation improves contractile force and delays fatigue in the soleusmuscles of diabetic rats. How to cite this: Khan BU, Yousef I, Arshad S, Ikram F, Ali A, Ullah I. Effects of Alpha Lipoic Acid Supplementation on Slow Skeletal Muscle Mass and Contractile Functions in Type 2 Diabetic Male Sprague Dawley Rats. Life and Science. 2024; 5(2): 216-221. doi: http://doi.org/10.37185/LnS.1.1.408

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补充α-硫辛酸对 2 型糖尿病雄性 Sprague Dawley 大鼠慢速骨骼肌质量和收缩功能的影响

研究目的观察补充α-硫辛酸（ALA）对2型糖尿病雄性Sprague Dawley大鼠骨骼肌慢肌质量和收缩功能的影响：准实验研究：该研究于2019年6月至2021年4月在巴基斯坦拉瓦尔品第陆军医学院生理研究实验室进行，并与巴基斯坦伊斯兰堡国家卫生研究院（NIH）合作：方法：将 60 只成年 SD 大鼠分为 3 组。大鼠按照 NIHprotocols（美国国立卫生研究院协议）的标准饮食喂养。2 周后，通过腹腔注射低剂量链脲佐菌素（STZ）35 毫克，在第 2 组和第 3 组诱发 2 型糖尿病（T2DM）。通过血糖仪测量血糖水平，成功诱发并确认了 T2DM。接下来的两周，第 3 组在下腹部注射阿尔法硫辛酸 30 毫克/千克/天。04周后，解剖比目鱼肌，使用动物数据采集装置（iWorx）评估比目鱼肌的收缩功能：结果：与第二组相比，阿尔法硫辛酸组在肌肉质量、肌肉张力强度和疲劳恢复方面均有改善：结论：补充α-硫辛酸可提高糖尿病大鼠比目鱼肌的收缩力并延缓疲劳。引用方式Khan BU, Yousef I, Arshad S, Ikram F, Ali A, Ullah I. Effects of Alpha Lipoic Acid Supplementation on Slow Skeletal Muscle Mass and Contractile Functions in Type 2 Diabetic Male Sprague Dawley Rats. Life and Science.生命与科学2024; 5(2):216-221. doi: http://doi.org/10.37185/LnS.1.1.408

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