Aptamer and phosphorothioated DNA engineered liposomes as a targeted intracellular protein delivery system

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL Chinese Journal of Analytical Chemistry Pub Date : 2024-06-01 DOI:10.1016/j.cjac.2024.100400

Jiamei LI , An YAN , Mingquan WANG , Di LI

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Abstract

Targeted delivery of proteins into desired cell groups is crucial for disease therapy and cellular functionalization. However, current delivery methods face severe side effects and off-target problems, making it difficult to achieve cell-targeted protein delivery. Herein, we developed a user-friendly membrane fusion liposome for cancer cell-targeted protein delivery. Phosphorothioated DNA-mediated membrane fusion was employed as an efficient transmembrane delivery approach. The phosphorothioated DNA was capped with the AS1411 aptamer, which specifically recognizes nucleolar proteins on the cancer cell membrane, enabling a controllable delivery of proteins into targeted cancer cells. This delivery system exhibited commendable biocompatibility and targeted delivery ability, thereby realizing highly effective cancer cell inhibition in vitro. The in vivo results further suggested that the membrane protein-responsive membrane fusion delivery system offers a new avenue for highly biocompatible and targeted protein delivery.

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色聚体和硫代磷酸 DNA 工程脂质体作为一种靶向细胞内蛋白质输送系统

将蛋白质靶向输送到所需的细胞群对于疾病治疗和细胞功能化至关重要。然而，目前的递送方法面临着严重的副作用和脱靶问题，很难实现细胞靶向蛋白质递送。在此，我们开发了一种便于使用的膜融合脂质体，用于癌细胞靶向蛋白递送。硫代磷酸 DNA 介导的膜融合是一种高效的跨膜递送方法。硫代磷酸 DNA 以 AS1411 aptamer 为封端，AS1411 aptamer 可特异性识别癌细胞膜上的核仁蛋白，从而可控地将蛋白质输送到靶向癌细胞中。这种递送系统具有良好的生物相容性和靶向递送能力，从而在体外实现了对癌细胞的高效抑制。体内研究结果进一步表明，膜蛋白响应膜融合输送系统为高生物相容性和靶向性蛋白质输送提供了一条新途径。

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.