Chinese Journal of Analytical Chemistry最新文献

Analytical strategies for quantifying methylated selenium species in staple crops: Methods, and emerging techniques

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-04-01 DOI: 10.1016/j.cjac.2025.100511

Angstone Thembachako MLANGENI , Fatema JAGOT , Sydney NAMAUMBO , Noel Jabesi KAPITO , Deborah Charles TSUKULUZA , Loti BOTHA , Patrick NDOVIE , Patsani KUMAMBALA

Background

Selenium is an essential trace element that exists in various forms, including methylated species, each with distinct biological activities and toxicity profiles. Understanding these transformations is critical for evaluating selenium's role in health and the environment. This review was conducted through a targeted search of multiple databases, including PubMed, Scopus, Web of Science, and Google Scholar. Keywords such as "selenium methylation detection" and "analytical methods for selenium" were used to gather a broad and relevant selection of articles. Studies were included based on their relevance, methodological quality, and contributions to the field.

Results

The review provides a detailed analysis of both established and emerging analytical techniques for selenium detection. This includes gas chromatography-mass spectrometry (GC–MS), liquid chromatography-mass spectrometry (LC-MS), high-performance liquid chromatography (HPLC), X-ray absorption spectroscopy (XAS), and nuclear magnetic resonance (NMR). It evaluates the effectiveness of these methods in separating, identifying, and quantifying selenium species, with a particular focus on sensitivity, selectivity, and throughput. Additionally, new technologies such as capillary electrophoresis coupled with inductively coupled plasma-mass spectrometry (CE-ICP-MS) and laser-induced breakdown spectroscopy (LIBS) are discussed for their potential to enhance precision and efficiency.

Significance

This review highlights the critical role of advanced analytical methods in understanding selenium transformations and improving measurement accuracy. By addressing challenges such as matrix effects, overlapping retention times, and spectral interferences, the review underscores the importance of reliable selenium analysis for health and environmental assessments, and provides insights into the ongoing advancements in analytical technology.

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引用次数: 0

Electrochemical surface modification and regeneration of carbon fiber microelectrodes in deionized water

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-25 DOI: 10.1016/j.cjac.2025.100531

Tao FANG , Tingting WANG , Uchkun ISHIMOV , Yuan LI , Ghulam MURTAZA , Awais IHSAN , Yanbing PAN , Ruiting TIAN , Han CHENG , Xianju HUANG

The contamination, passivation, or fouling of the detection electrodes is a serious problem undermining the analytical performance of electroanalytical devices. In this paper, a simple and effective carbon fiber microelectrodes (CFME) renewal method was proposed for the first time. CFME was pretreated with an initial potential of 1.75 V in deionized water for 26.13 min to obtain an activated electrode with a significantly increased electrochemical response to dopamine. This method can restore the electrochemical performance of the inactivated CFME. An advantage of this method is that its activation and regeneration effect on the electrode was not less than the other reported activation solutions, although deionized water does not contain any other electrolyte. Our study furthered the understanding of the activation mechanism of CFME treated electrochemically in deionized water, this was ascribed to a renewing in the surface of carbon fibers after electrochemical treatment. At the same time, it was proved that the oxygen-containing functional groups were modified on the surface of activated CFME, which led to the regeneration of an electrochemically active surface and improved CFME sensitivity. The differential pulse voltammetry (DPV) response of dopamine showed good linearity (R² = 0.9961) on the regenerated CFME in the concentration range from 1.0 × 10⁻⁷ to 1.0 × 10⁻⁴ mol/L. A limit of detection as low as 3.1 × 10^–8 mol/L was found. The regenerated CFME was quite stable and had good reproducibility. It is expected to be applied to activate and regenerate electrodes contaminated in the monitoring of a complex biological microenvironment.

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引用次数: 0

Characterization and Health - related Study of Maifanitum by ATR - FTIR and ICP - MS

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-19 DOI: 10.1016/j.cjac.2025.100529

Xilong QIAN , Sicheng WU , Liu ZHOU , Zheng LIU , Yanqiong PAN , Xiangsong MENG , YuLu MA , Fang FANG , Kewei WANG , Jing ZHAO , Shengjin LIU

With the continuous rise of consumers' material and physical health pursuit, it is particularly critical to evaluate the safety and effectiveness of natural healthy mineral materials such as Maifanitum. The progress of modern analytical technology enables us to deeply study the internal characteristics and mechanism of these traditional mineral materials, providing a solid scientific foundation and technical support for the application of Maifanitum. A comprehensive analysis of Maifanitum was conducted using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and inductively coupled plasma mass spectrometry (ICP-MS). The ATR-FTIR analysis established a quality control fingerprint for Maifanitum, and the similarity evaluation method was employed to assess the similarity between different samples. Orthogonal partial least squares discriminant analysis (OPLS-DA) analysis revealed distinct absorption peaks among Maifanitum samples from different production areas, enabling traceability and identification. ICP-MS technology was utilized to analyze the inorganic elements in Maifanitum, revealing numerous elements closely associated with human health. These findings indicate that the established ATR-FTIR fingerprint can be effectively used to identify and control the quality of Maifanitum. The characteristics of inorganic elements in Maifanitum can not only help to distinguish its sources, but also provide a theoretical basis for the current application of Maifanitum in health care.

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引用次数: 0

Deciphering the mechanism of Sang Ju Yin in ameliorating acute lung injury: An integrated systems pharmacology approach encompassing chemical composition analysis, network pharmacology, metabolomics, molecular docking and molecular biology 解密桑菊饮改善急性肺损伤的机制：包含化学成分分析、网络药理学、代谢组学、分子对接和分子生物学的综合系统药理学方法

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-11 DOI: 10.1016/j.cjac.2025.100527

Ruinan REN , Fang WANG , Guoan ZHAO , Qi YU , Jinling ZHANG , Wenbao WANG , Tianyang WANG , Song LIN , Yan LIN

Sang Ju Yin (SJY), a renowned traditional Chinese medicinal formula, has been widely utilized in acute lung injury (ALI) management. Yet, its underlying therapeutic mechanisms remain obscure. This study devised an innovative and integrated methodology, merging advanced chemical analysis with biological assays, to elucidate SJY's action mechanism in ALI treatment. Initially, UFLC-ESI-QTOF-MS was employed to precisely analyze SJY's chemical constituents. Subsequently, network pharmacology predicted potential targets and signaling pathways for SJY's beneficial effects. In parallel, pharmacodynamic evaluation was performed on ALI rats. Utilizing LC-MS and ¹H NMR metabolomics techniques with an innovative data fusion strategy, potential biomarkers and perturbed metabolic pathways were identified. Crucially, integrating network pharmacology and metabolomics insights yielded a holistic understanding of the mechanism. Finally, verification experiments involving molecular docking, Western Blot, and qRT-PCR were carried out. Notably, potential key bioactive components including apigenin were identified for SJY's anti-ALI activity. Marked perturbations in representative pathways such as arachidonic acid (AA) metabolism and PI3K-Akt pathway were obtained after SJY administration. Furthermore, integrated data spotlighted PTGS2, PLA2, and AA metabolism as pivotal, linking predicted targets and metabolic alterations for SJY in ALI treatment. In summary, this study uncovers SJY's mechanism in ALI treatment, presenting an advanced interdisciplinary framework. It deepens our comprehension of traditional Chinese medicine's role in ALI, setting a new standard for research at the chemistry-pharmacology interface.

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引用次数: 0

A selective methane gas sensor based on SnO2 utilizing a reactive and porous substrate

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-06 DOI: 10.1016/j.cjac.2025.100526

Youzhi LUO , Jiaqi GAO , Huihua FU , Shuhuan LIU , Zhongqiu HUA

Generally, selective response of metal oxide semiconductor (MOS) gas sensors is dominated by the process of gas diffusion and reactions through the sensing layers. The microstructure and surface chemical activities of MOS thin films simultaneously control the diffusion reaction process and modulate electronic conduction. Thus, it is very difficult to modulate the diffusion-reaction process just using sensing layers because gas diffusion and reaction process cannot be separated from the sensing films. Hence, the selective response of the MOS gas sensors is barely satisfactory. In this study, a novel sensor structure was proposed. The MOS sensing layer was printed on a porous ceramic substrate with a well-defined pore structure. The gas molecules must diffuse through the porous substrate before contacting the sensing layer of the MOS. Therefore, the diffusion reaction and sensing processes are separated from each other and are performed by two different parts of the sensor devices. Consequently, the selective response was significantly improved by the proposed sensor structure.

{"title":"A selective methane gas sensor based on SnO2 utilizing a reactive and porous substrate","authors":"Youzhi LUO , Jiaqi GAO , Huihua FU , Shuhuan LIU , Zhongqiu HUA","doi":"10.1016/j.cjac.2025.100526","DOIUrl":"10.1016/j.cjac.2025.100526","url":null,"abstract":"<div><div>Generally, selective response of metal oxide semiconductor (MOS) gas sensors is dominated by the process of gas diffusion and reactions through the sensing layers. The microstructure and surface chemical activities of MOS thin films simultaneously control the diffusion reaction process and modulate electronic conduction. Thus, it is very difficult to modulate the diffusion-reaction process just using sensing layers because gas diffusion and reaction process cannot be separated from the sensing films. Hence, the selective response of the MOS gas sensors is barely satisfactory. In this study, a novel sensor structure was proposed. The MOS sensing layer was printed on a porous ceramic substrate with a well-defined pore structure. The gas molecules must diffuse through the porous substrate before contacting the sensing layer of the MOS. Therefore, the diffusion reaction and sensing processes are separated from each other and are performed by two different parts of the sensor devices. Consequently, the selective response was significantly improved by the proposed sensor structure.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 5","pages":"Article 100526"},"PeriodicalIF":1.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic fingerprints of protein corona formation on MIL-88B(Fe)-NH2 metal-organic framework

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-06 DOI: 10.1016/j.cjac.2025.100524

Junyu YE , Yanhong LI , Yang GAO , Yiling LI , Bin XIA , Zhengguo CUI , Keming QU

When nanoparticles are introduced to living systems, they are quickly covered by a biomolecular coating referred to as the protein corona. This coating endows nanoparticles with new biorecognition properties, subsequently impacting their behavior and downstream toxic effects. Herein, the proteomic fingerprints of protein corona formation on Fe-based metal-organic framework (Fe-MOF) MIL-88B(Fe)-NH₂ exposed to plasma of the marine flatfish turbot (Scophthalmus maximus) were investigated. The formation of fish plasma protein corona on haemocompatibility of Fe-MOF were investigated. Transmission electron microscopy and flow cytometry confirmed the formation of plasma protein coronas on the Fe-MOF particles. The immunoglobulins and complement proteins were enriched on the Fe-MOF surface, whereas the lipoproteins and coagulation proteins were significantly depleted compared with their original concentrations in the crude plasma. Hemolysis induced by Fe-MOF was influenced by fish plasma protein corona formation. This study offers insights into the interaction between a representative Fe-MOF and fish plasma proteins, enabling a better understanding of the biological behavior of Fe-MOFs in the blood system.

{"title":"Proteomic fingerprints of protein corona formation on MIL-88B(Fe)-NH2 metal-organic framework","authors":"Junyu YE , Yanhong LI , Yang GAO , Yiling LI , Bin XIA , Zhengguo CUI , Keming QU","doi":"10.1016/j.cjac.2025.100524","DOIUrl":"10.1016/j.cjac.2025.100524","url":null,"abstract":"<div><div>When nanoparticles are introduced to living systems, they are quickly covered by a biomolecular coating referred to as the protein corona. This coating endows nanoparticles with new biorecognition properties, subsequently impacting their behavior and downstream toxic effects. Herein, the proteomic fingerprints of protein corona formation on Fe-based metal-organic framework (Fe-MOF) MIL-88B(Fe)-NH<sub>2</sub> exposed to plasma of the marine flatfish turbot (<em>Scophthalmus maximus</em>) were investigated. The formation of fish plasma protein corona on haemocompatibility of Fe-MOF were investigated. Transmission electron microscopy and flow cytometry confirmed the formation of plasma protein coronas on the Fe-MOF particles. The immunoglobulins and complement proteins were enriched on the Fe-MOF surface, whereas the lipoproteins and coagulation proteins were significantly depleted compared with their original concentrations in the crude plasma. Hemolysis induced by Fe-MOF was influenced by fish plasma protein corona formation. This study offers insights into the interaction between a representative Fe-MOF and fish plasma proteins, enabling a better understanding of the biological behavior of Fe-MOFs in the blood system.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 5","pages":"Article 100524"},"PeriodicalIF":1.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive determination of amphetamine-type stimulants in human hair by electro-enhanced solid-phase microextraction coupled to gas chromatography with nitrogen-phosphorus detector 电增强固相微萃取-气相色谱-氮磷检测器灵敏测定人类毛发中的苯丙胺类兴奋剂

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-06 DOI: 10.1016/j.cjac.2025.100525

Aiying SONG , Rong LIU , Xinghe HE , Linlin WEI

In the field of forensic sciences, the analysis of amphetamine-type stimulants in hair samples has become a fundamental tool for assessing long-term substance use. The persistent nature of amphetamine-type stimulants in hair provides a unique chronological record of drug intake, offering valuable insights for legal and clinical applications. Developing a simple, sensitive, and reliable method for the determination of these drugs in hair samples remains an important issue in forensic analysis. In this study, polydimethylsiloxane modified stainless steel fiber was firstly fabricated. By taking advantage of the affinity of the polydimethylsiloxane coating toward amphetamine-type stimulants and the excellent conductivity of stainless steel wire, an electro-enhanced solid-phase microextraction coupled with gas chromatography equipped with a nitrogen-phosphorus detector method was established to sensitively determine four common amphetamine-type stimulants in hair samples. The experimental results showed a linear range from 0.1 to 50 µg L⁻¹. The limits of detection and qualification respectively varied from 0.012 to 0.039 and 0.05 to 0.13 µg L⁻¹ (S/N = 3), and it exhibited good reproducibility (< 6.2%). Recoveries ranging from 79% to 106%, with a relative standard deviation of < 6.7%, were obtained for all analytes. Fiber-to-fiber reproducibility (RSD), obtained using three fibers, was < 8.8%. The developed strategy combined the affinity of the polydimethylsiloxane coating towards amphetamine-type stimulants with the electrical conductivity of the stainless steel wire, effectively improving the enrichment ability and detection sensitivity, and achieving simultaneous selective enrichment and detection of several analytes. This is a simple, efficient, and reliable method for the analysis of amphetamine-type stimulants from complex matrices.

在法医学领域，分析毛发样本中的苯丙胺类兴奋剂已成为评估长期药物使用情况的基本工具。苯丙胺类兴奋剂在毛发中的持久性为药物摄入提供了独特的时间记录，为法律和临床应用提供了宝贵的见解。开发一种简单、灵敏、可靠的方法来测定毛发样本中的这类药物仍然是法医分析中的一个重要问题。本研究首先制备了聚二甲基硅氧烷改性不锈钢纤维。利用聚二甲基硅氧烷涂层对苯丙胺类兴奋剂的亲和性和不锈钢丝良好的导电性，建立了电增强固相微萃取-气相色谱-氮磷检测器联用方法，灵敏地测定了毛发样品中四种常见的苯丙胺类兴奋剂。实验结果表明，其线性范围为 0.1 至 50 µg L-¹。检出限和定性限分别为 0.012 至 0.039 和 0.05 至 0.13 µg L-¹（信噪比为 3），重现性良好（6.2%）。所有分析物的回收率为 79% 至 106%，相对标准偏差为 6.7%。使用三根纤维获得的纤维间重现性（RSD）为 8.8%。所开发的策略将聚二甲基硅氧烷涂层对苯丙胺类兴奋剂的亲和性与不锈钢丝的导电性相结合，有效提高了富集能力和检测灵敏度，实现了对多种分析物的同时选择性富集和检测。该方法简单、高效、可靠，适用于复杂基质中苯丙胺类兴奋剂的分析。

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引用次数: 0

Development of a new eco-friendly HPLC method and assessing the pharmacokinetics of pelitinib, a potent epidermal growth factor receptor inhibitor used for the treatment of non-small cell lung cancer associated with EML4-ALK mutant gene

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-01 DOI: 10.1016/j.cjac.2025.100513

Rashed N. HERQASH , Ali S. ALQAHTANI , Ibrahim A. DARWISH

Pelitinib (PEL) is a potent drug which demonstrated clinical success in the treatment of non-small cell lung cancers harboring the mutant fusion gene EML4-ALK (echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase. The objective of this study was the development of eco-friendly HPLC method to refine the therapeutic findings, support pharmacokinetic assessments, and aid in the potential therapeutic monitoring of PEL. The chromatographic separation of PEL and levofloxacin as an internal standard (IS) was accomplished on a Shim-pack VP-ODS C18 HPLC column using a gradient elution with a mobile phase consisting of formic acid (0.1 %, v/v) in an acetonitrile–methanol mixture (80:20 %, v/v). The flow rate was 1.0 mL min^–1 and the detection wavelength was 260 nm. Validation of the method was conducted in accordance with ICH guidelines for bioanalytical method validation, and all parameters’ values were acceptable. The novelty of the proposed eco-friendly HPLC-UV method in terms of its adherence of the method's procedures to the requirements of the green analytical chemistry approaches was confirmed by three comprehensive metric tools. The method was successfully applied in studying the pharmacokinetics of PEL in rats. The findings revealed that PEL was absorbed and reached maximum plasma concentration of 182.08 ng mL⁻¹ after 4 h and had an eliminated rate constant of 0.072 h⁻¹. The volume of distribution was 0.064 L/kg. The clearance was found at 0.005 L/h/kg. The results presented this method as a valuable tool for realizing targeted therapeutic benefits and ensuring safety of PEL treatment.

{"title":"Development of a new eco-friendly HPLC method and assessing the pharmacokinetics of pelitinib, a potent epidermal growth factor receptor inhibitor used for the treatment of non-small cell lung cancer associated with EML4-ALK mutant gene","authors":"Rashed N. HERQASH , Ali S. ALQAHTANI , Ibrahim A. DARWISH","doi":"10.1016/j.cjac.2025.100513","DOIUrl":"10.1016/j.cjac.2025.100513","url":null,"abstract":"<div><div>Pelitinib (PEL) is a potent drug which demonstrated clinical success in the treatment of non-small cell lung cancers harboring the mutant fusion gene EML4-ALK (echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase. The objective of this study was the development of eco-friendly HPLC method to refine the therapeutic findings, support pharmacokinetic assessments, and aid in the potential therapeutic monitoring of PEL. The chromatographic separation of PEL and levofloxacin as an internal standard (IS) was accomplished on a Shim-pack VP-ODS C18 HPLC column using a gradient elution with a mobile phase consisting of formic acid (0.1 %, v/v) in an acetonitrile–methanol mixture (80:20 %, v/v). The flow rate was 1.0 mL min<sup>–1</sup> and the detection wavelength was 260 nm. Validation of the method was conducted in accordance with ICH guidelines for bioanalytical method validation, and all parameters’ values were acceptable. The novelty of the proposed eco-friendly HPLC-UV method in terms of its adherence of the method's procedures to the requirements of the green analytical chemistry approaches was confirmed by three comprehensive metric tools. The method was successfully applied in studying the pharmacokinetics of PEL in rats. The findings revealed that PEL was absorbed and reached maximum plasma concentration of 182.08 ng mL<sup>−1</sup> after 4 h and had an eliminated rate constant of 0.072 h<sup>−1</sup>. The volume of distribution was 0.064 L/kg. The clearance was found at 0.005 L/h/kg. The results presented this method as a valuable tool for realizing targeted therapeutic benefits and ensuring safety of PEL treatment.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 3","pages":"Article 100513"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptosis-inducing effects of aqueous extract of Eleutherococcus senticosus on non-small cell lung cancer cell proliferation

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-01 DOI: 10.1016/j.cjac.2025.100510

Xuekun KOU , Yufeng LI , Lei WANG , Xin SONG , Dan LI , Zhuo WANG , Yuanyuan ZHAO , Xiaohui ZHANG , Jingwu LI , Zhaobin XING

Non-small cell lung cancer is a malignant tumor with high morbidity and mortality worldwide. Eleutherococcus senticosus can induce apoptosis in non-small cell lung cancer cells, but the mechanism remains unclear. This study aimed to elucidate the role of Eleutherococcus senticosus in inducing apoptosis in non-small cell lung cancer cells and analyze its potential active constituents, targets, and molecular mechanisms. The results of network pharmacology analysis showed that Eleutherococcus senticosus contained 49 active ingredients that induced apoptosis in non-small cell lung cancer cells, and these components could act on 66 apoptosis-related targets. Compared to the control group, Eleutherococcus senticosus significantly increased apoptosis in A549 cells with increasing concentration (p < 0.05). The results of transcriptome and metabolomic analyses showed that Eleutherococcus senticosus significantly changed 5836 genes and 418 metabolites in A549 cells (p < 0.05), with the most significant changes in 18 genes and 34 metabolites related to apoptosis. qRT-PCR and Western blot results showed that, after Eleutherococcus senticosus treatment, the mRNA and protein expression of EGFR, MAPK3, and ICAM1 significantly increased, while CTSK decreased (p < 0.01 or p < 0.001). Correlation analysis and molecular docking results indicated that calycanthoside and oleanolic acid can directly modify the expression levels of the transcription factors POU2F3, FOXS1, and TGIF2LY or indirectly influence the binding affinity of these transcription factors to the promoters of key target genes, ultimately leading to the activation of EGFR, MAPK3, ICAM1, and CTSK, which triggers apoptosis in non-small cell lung cancer cells.

{"title":"Apoptosis-inducing effects of aqueous extract of Eleutherococcus senticosus on non-small cell lung cancer cell proliferation","authors":"Xuekun KOU , Yufeng LI , Lei WANG , Xin SONG , Dan LI , Zhuo WANG , Yuanyuan ZHAO , Xiaohui ZHANG , Jingwu LI , Zhaobin XING","doi":"10.1016/j.cjac.2025.100510","DOIUrl":"10.1016/j.cjac.2025.100510","url":null,"abstract":"<div><div>Non-small cell lung cancer is a malignant tumor with high morbidity and mortality worldwide. <em>Eleutherococcus senticosus</em> can induce apoptosis in non-small cell lung cancer cells, but the mechanism remains unclear. This study aimed to elucidate the role of <em>Eleutherococcus senticosus</em> in inducing apoptosis in non-small cell lung cancer cells and analyze its potential active constituents, targets, and molecular mechanisms. The results of network pharmacology analysis showed that <em>Eleutherococcus senticosus</em> contained 49 active ingredients that induced apoptosis in non-small cell lung cancer cells, and these components could act on 66 apoptosis-related targets. Compared to the control group, <em>Eleutherococcus senticosus</em> significantly increased apoptosis in A549 cells with increasing concentration (<em>p <</em> 0.05). The results of transcriptome and metabolomic analyses showed that <em>Eleutherococcus senticosus</em> significantly changed 5836 genes and 418 metabolites in A549 cells (<em>p <</em> 0.05), with the most significant changes in 18 genes and 34 metabolites related to apoptosis. qRT-PCR and Western blot results showed that, after <em>Eleutherococcus senticosus</em> treatment, the mRNA and protein expression of <em>EGFR, MAPK3</em>, and <em>ICAM1</em> significantly increased, while <em>CTSK</em> decreased (<em>p <</em> 0.01 or <em>p <</em> 0.001). Correlation analysis and molecular docking results indicated that calycanthoside and oleanolic acid can directly modify the expression levels of the transcription factors <em>POU2F3, FOXS1</em>, and <em>TGIF2LY</em> or indirectly influence the binding affinity of these transcription factors to the promoters of key target genes, ultimately leading to the activation of <em>EGFR, MAPK3, ICAM1</em>, and <em>CTSK</em>, which triggers apoptosis in non-small cell lung cancer cells.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 3","pages":"Article 100510"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplemented Xijiao Dihuang Decoction alleviates sepsis via modulation of gut microbiota and metabolites: A multi-omics approach

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-02-24 DOI: 10.1016/j.cjac.2025.100517

Aiping ZHANG , Xingxing HU , Zhenfeng LU , Haibin NI , Jingsheng GUO , Xiaofei HUANG , Yehong HU , Xiaoming YAO , Zhijun FANG , Lei WANG

Sepsis, a critical condition that is a leading cause of mortality in critically ill patients, involves complex interactions between host genetics and environmental factors. The present study evaluated the therapeutic efficacy and mechanisms of action of supplemented Xijiao Dihuang Decoction (SXJDHD), a traditional Chinese medicine (TCM) formula augmented with additional herbs, in treating sepsis. Using a multi-omics approach encompassing metabolomics and gut microbiota analysis, we investigated the effects of SXJDHD on sepsis outcomes. Network pharmacology analysis revealed that SXJDHD targets multiple pathways implicated in sepsis pathogenesis. In a mouse model of sepsis, SXJDHD significantly improved survival rates, alleviated multi-organ damage, and reduced the levels of inflammatory cytokines TNF-α and IL-6. Additionally, SXJDHD modulated the gut microbiota, increasing the abundance of beneficial bacteria such as Bacteroides and Prevotellaceae UCG-001, while decreasing that of Helicobacter. Metabolomics analysis showed significant changes in microbial metabolites following SXJDHD intervention, suggesting modulation of the metabolome. Collectively, these findings indicate that SXJDHD exhibits therapeutic potential in sepsis through the regulation of gut microbiota and metabolites, providing insights into the mechanisms underlying its efficacy.

败血症是一种危重症，是危重症患者死亡的主要原因，涉及宿主遗传和环境因素之间复杂的相互作用。本研究评估了补益西郊地黄汤（SXJDHD）治疗败血症的疗效和作用机制。我们采用包括代谢组学和肠道微生物群分析在内的多组学方法，研究了 SXJDHD 对败血症结果的影响。网络药理学分析表明，SXJDHD 可靶向与败血症发病机制有关的多条通路。在脓毒症小鼠模型中，SXJDHD能显著提高存活率，减轻多器官损伤，并降低炎性细胞因子TNF-α和IL-6的水平。此外，SXJDHD 还能调节肠道微生物群，增加有益菌（如 Bacteroides 和 Prevotellaceae UCG-001）的数量，同时减少螺旋杆菌的数量。代谢组学分析表明，SXJDHD干预后微生物代谢物发生了显著变化，表明代谢组发生了改变。总之，这些研究结果表明，SXJDHD 通过调节肠道微生物群和代谢物对败血症具有治疗潜力，为了解其疗效机制提供了启示。

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引用次数: 0