Phosphorylation of ZmAL14 by ZmSnRK2.2 regulates drought resistance through derepressing ZmROP8 expression

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Integrative Plant Biology Pub Date : 2024-05-28 DOI:10.1111/jipb.13677
Yalin Wang, Jinkui Cheng, Yazhen Guo, Zhen Li, Shuhua Yang, Yu Wang, Zhizhong Gong
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Abstract

Drought stress has negative effects on crop growth and production. Characterization of transcription factors that regulate the expression of drought-responsive genes is critical for understanding the transcriptional regulatory networks in response to drought, which facilitates the improvement of crop drought tolerance. Here, we identified an Alfin-like (AL) family gene ZmAL14 that negatively regulates drought resistance. Overexpression of ZmAL14 exhibits susceptibility to drought while mutation of ZmAL14 enhances drought resistance. An abscisic acid (ABA)-activated protein kinase ZmSnRK2.2 interacts and phosphorylates ZmAL14 at T38 residue. Knockout of ZmSnRK2.2 gene decreases drought resistance of maize. A dehydration-induced Rho-like small guanosine triphosphatase gene ZmROP8 is directly targeted and repressed by ZmAL14. Phosphorylation of ZmAL14 by ZmSnRK2.2 prevents its binding to the ZmROP8 promoter, thereby releasing the repression of ZmROP8 transcription. Overexpression of ZmROP8 stimulates peroxidase activity and reduces hydrogen peroxide accumulation after drought treatment. Collectively, our study indicates that ZmAL14 is a negative regulator of drought resistance, which can be phosphorylated by ZmSnRK2.2 through the ABA signaling pathway, thus preventing its suppression on ZmROP8 transcription during drought stress response.

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ZmSnRK2.2对ZmAL14的磷酸化通过抑制ZmROP8的表达来调节抗旱性。
干旱胁迫对作物生长和产量有负面影响。表征调控干旱响应基因表达的转录因子对于了解干旱响应的转录调控网络至关重要,有助于提高作物的抗旱性。在这里,我们发现了一种能负向调控抗旱性的类阿尔芬(AL)家族基因 ZmAL14。ZmAL14的过表达表现出对干旱的易感性,而ZmAL14的突变则增强了抗旱性。脱落酸(ABA)激活的蛋白激酶ZmSnRK2.2与ZmAL14相互作用并使其在T38残基上磷酸化。敲除 ZmSnRK2.2 基因会降低玉米的抗旱性。脱水诱导的Rho样小鸟苷三磷酸酶基因ZmROP8被ZmAL14直接靶向并抑制。ZmSnRK2.2对ZmAL14的磷酸化阻止了它与ZmROP8启动子的结合,从而解除了对ZmROP8转录的抑制。过表达 ZmROP8 能刺激过氧化物酶的活性,减少干旱处理后过氧化氢的积累。综上所述,我们的研究表明,ZmAL14是抗旱性的负调控因子,它可以通过ABA信号途径被ZmSnRK2.2磷酸化,从而阻止其在干旱胁迫响应过程中对ZmROP8转录的抑制。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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