Ziao Jiao, Mingliang Pan, Khadija Yousaf, Daniel Doveiko, Michelle Maclean, David Griffin, Yu Chen, David Day Uei Li
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引用次数: 0
Abstract
We propose a smartphone-based optical sectioning (SOS) microscope based on the HiLo technique, with a single smartphone replacing a high-cost illumination source and a camera sensor. We built our SOS with off-the-shelf optical, mechanical cage systems with 3D-printed adapters to seamlessly integrate the smartphone with the SOS main body. The liquid light guide can be integrated with the adapter, guiding the smartphone's LED light to the digital mirror device (DMD) with neglectable loss. We used an electrically tuneable lens (ETL) instead of a mechanical translation stage to realise low-cost axial scanning. The ETL was conjugated to the objective lens's back pupil plane (BPP) to construct a telecentric design by a 4f configuration to maintain the lateral magnification for different axial positions. SOS has a 571.5 µm telecentric scanning range and an 11.7 µm axial resolution. The broadband smartphone LED torch can effectively excite fluorescent polystyrene (PS) beads. We successfully used SOS for high-contrast fluorescent PS beads imaging with different wavelengths and optical sectioning imaging of multilayer fluorescent PS beads. To our knowledge, the proposed SOS is the first smartphone-based HiLo optical sectioning microscopy (£1965), which can save around £7035 compared with a traditional HiLo system (£9000). It is a powerful tool for biomedical research in resource-limited areas.
我们提出了一种基于智能手机的光学切片(SOS)显微镜,它以 HiLo 技术为基础,用一部智能手机取代了高成本的照明光源和相机传感器。我们利用现成的光学、机械笼系统和 3D 打印适配器构建了 SOS,将智能手机与 SOS 主体无缝集成。液态光导可与适配器集成,以可忽略的损耗将智能手机的 LED 光导至数字镜像设备(DMD)。我们使用电动可调透镜(ETL)代替机械平移台,以实现低成本的轴向扫描。ETL 与物镜的后瞳孔平面 (BPP) 连接,通过 4f 配置构建远心设计,以保持不同轴向位置的横向放大率。SOS 的远心扫描范围为 571.5 微米,轴向分辨率为 11.7 微米。宽带智能手机 LED 手电筒可有效激发荧光聚苯乙烯(PS)珠。我们成功地将 SOS 用于不同波长的高对比度荧光聚苯乙烯珠成像和多层荧光聚苯乙烯珠的光学切片成像。据我们所知,拟议的 SOS 是首个基于智能手机的 HiLo 光学切片显微镜(1965 英镑),与传统 HiLo 系统(9000 英镑)相比,可节省约 7035 英镑。它是资源有限地区生物医学研究的有力工具。
期刊介绍:
The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit.
The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens.
Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.