Live imaging analysis of sexual and asexual reproduction, zygospore and sporangiospore formation, in Gilbertella persicaria

IF 1.5 4区生物学 Q4 MYCOLOGY Mycoscience Pub Date : 2024-05-29 DOI:10.47371/mycosci.2024.03.002

Norio Takeshita, Yusuke Takashima, Yousuke Degawa

{"title":"Live imaging analysis of sexual and asexual reproduction, zygospore and sporangiospore formation, in Gilbertella persicaria","authors":"Norio Takeshita, Yusuke Takashima, Yousuke Degawa","doi":"10.47371/mycosci.2024.03.002","DOIUrl":null,"url":null,"abstract":"Most Mucoromycota fungi form zygospores as sexual reproductive structures. When two colonies of compatible strains meet, zygospores are formed in the area where the colonies meet. The structure and development of zygospores have been studied for a long time by light microscopy and electron microscopy. This study is the first time-lapse report on the dynamic movements of sexual and asexual reproductive processes by live imaging in Gilbertella persicaria (Choanephoraceae, Mucorales). Our live imaging analysis indicated the formation of zygospores begin immediately after two aerial hyphae contact whether at the tip or middle of the hyphae. The early-stage zygospores elongated from the contact site with a rate of 1.2–1.7 μm/s and reach < 200 μm in 2–3 h. Following maturation of zygospores, from progametangia to gametangia and maturation stage, took a few hours, in total 5 to 6 h after the first contact of two hyphae. When a zygospore was formed near the tip of hypha in contact with the partner hypha, the hyphal growth ceased. When zygospore was formed behind the tip of the hypha, the hyphal growth continued without slowing down. This study provides quantitative spatio-temporal information on the dynamics of zygospore formation.\n","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"44 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycoscience","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.47371/mycosci.2024.03.002","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Most Mucoromycota fungi form zygospores as sexual reproductive structures. When two colonies of compatible strains meet, zygospores are formed in the area where the colonies meet. The structure and development of zygospores have been studied for a long time by light microscopy and electron microscopy. This study is the first time-lapse report on the dynamic movements of sexual and asexual reproductive processes by live imaging in Gilbertella persicaria (Choanephoraceae, Mucorales). Our live imaging analysis indicated the formation of zygospores begin immediately after two aerial hyphae contact whether at the tip or middle of the hyphae. The early-stage zygospores elongated from the contact site with a rate of 1.2–1.7 μm/s and reach < 200 μm in 2–3 h. Following maturation of zygospores, from progametangia to gametangia and maturation stage, took a few hours, in total 5 to 6 h after the first contact of two hyphae. When a zygospore was formed near the tip of hypha in contact with the partner hypha, the hyphal growth ceased. When zygospore was formed behind the tip of the hypha, the hyphal growth continued without slowing down. This study provides quantitative spatio-temporal information on the dynamics of zygospore formation.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

对宿主吉尔伯特氏菌的有性生殖和无性生殖、子孢子和孢子囊形成的实时成像分析

大多数粘菌纲真菌都会形成子囊孢子作为有性生殖结构。当两个相容菌株的菌落相遇时，就会在菌落相遇的地方形成子囊孢子。长期以来，人们一直通过光学显微镜和电子显微镜研究子囊孢子的结构和发育。本研究首次通过活体成像技术，对柿树吉尔伯特菌（Choanephoraceae，Mucorales）有性生殖和无性生殖过程的动态运动进行了延时报道。我们的活体成像分析表明，无论是在菌丝的顶端还是中部，当两条气生菌丝接触后，子囊孢子立即开始形成。早期的子囊孢子以每秒 1.2-1.7 μm 的速度从接触部位开始伸长，2-3 小时后就能达到 < 200 μm。当子孢子在与伴侣菌丝接触的菌丝顶端附近形成时，菌丝生长停止。当子孢子在菌丝顶端后方形成时，菌丝生长继续，没有减缓。这项研究提供了关于子囊孢子形成动态的定量时空信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Mycoscience MYCOLOGY-

CiteScore

3.00

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.