Enhancing cellulose hydrolysis via cellulase immobilization on zeolitic imidazolate frameworks using physical adsorption.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-07-01 Epub Date: 2024-05-30 DOI:10.1007/s00449-024-03030-3

Liqun Sun, Chaozhong Xu, Shanshan Tong, Xiaoli Gu

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Abstract

This study investigates the immobilization of cellulase on zeolitic imidazolate frameworks (ZIFs) by physical adsorption, specifically the ZIF-8-NH₂ and Fe₃O₄@ZIF-8-NH₂, to enhance enzymatic hydrolysis efficiency. The immobilization process was thoroughly analyzed, including optimization of conditions and characterization of ZIF carriers and immobilized enzymes. The impacts on the catalytic activity of cellulase under various temperatures, pH levels, and storage conditions were examined. Additionally, the reusability of the immobilized enzyme was assessed. Results showed the cellulase immobilized on Fe₃O₄@ZIF-8-NH₂ exhibited a high loading capacity of 339.64 mg/g, surpassing previous studies. Its relative enzymatic activity was found to be 71.39%. Additionally, this immobilized enzyme system demonstrates robust reusability, retaining 68.42% of its initial activity even after 10 cycles. These findings underscore the potential of Fe₃O₄@ZIF-8-NH₂ as a highly efficient platform for cellulase immobilization, with promising implications for lignocellulosic biorefinery.

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利用物理吸附技术将纤维素酶固定在沸石咪唑酸盐框架上，从而提高纤维素水解效率。

本研究探讨了通过物理吸附将纤维素酶固定在沸石咪唑框架（ZIF）上，特别是 ZIF-8-NH2 和 Fe3O4@ZIF-8-NH2，以提高酶水解效率。研究人员对固定化过程进行了深入分析，包括优化条件、表征 ZIF 载体和固定化酶。研究了在不同温度、pH 值和储存条件下对纤维素酶催化活性的影响。此外，还评估了固定化酶的可重复使用性。结果表明，固定在 Fe3O4@ZIF-8-NH2 上的纤维素酶的负载能力高达 339.64 mg/g，超过了之前的研究。其相对酶活性为 71.39%。此外，这种固定化酶系统还具有很强的可重复使用性，即使在循环 10 次后，其初始活性仍能保持 68.42%。这些发现强调了 Fe3O4@ZIF-8-NH2 作为高效纤维素酶固定化平台的潜力，对木质纤维素生物炼制具有广阔的前景。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.