Quorum Quenching Potential of Reyranella sp. Isolated from Riverside Soil and Description of Reyranella humidisoli sp. nov.

IF 3.3 4区 生物学 Q2 MICROBIOLOGY Journal of Microbiology Pub Date : 2024-06-01 Epub Date: 2024-05-30 DOI:10.1007/s12275-024-00131-2
Dong Hyeon Lee, Seung Bum Kim
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Abstract

Quorum quenching refers to any mechanism that inhibits quorum sensing processes. In this study, quorum quenching activity among bacteria inhabiting riverside soil was screened, and a novel Gram-stain-negative, rod shaped bacterial strain designated MMS21-HV4-11T, which showed the highest level of quorum quenching activity, was isolated and subjected to further analysis. Strain MMS21-HV4-11T could be assigned to the genus Reyranella of Alphaproteobacteria based on the 16S rRNA gene sequence, as the strain shared 98.74% sequence similarity with Reyranella aquatilis seoho-37T, and then 97.87% and 97.80% sequence similarity with Reyranella soli KIS14-15T and Reyranella massiliensis 521T, respectively. The decomposed N-acyl homoserine lactone was restored at high concentrations under acidic conditions, implying that lactonase and other enzyme(s) are responsible for quorum quenching. The genome analysis indicated that strain MMS21-HV4-11T had two candidate genes for lactonase and one for acylase, and expected protein structures were confirmed. In the quorum sensing inhibition assay using a plant pathogen Pectobacterium carotovorum KACC 14888, development of soft rot was significantly inhibited by strain MMS21-HV4-11T. Besides, the swarming motility by Pseudomonas aeruginosa PA14 was significantly inhibited in the presence of strain MMS21-HV4-11T. Since the isolate did not display direct antibacterial activity against either of these species, the inhibition was certainly due to quorum quenching activity. In an extended study with the type strains of all known species of Reyranella, all strains were capable of degrading N-acyl homoserine lactones (AHLs), thus showing quorum quenching potential at the genus level. This is the first study on the quorum quenching potential and enzymes responsible in Reyranella. In addition, MMS21-HV4-11T could be recognized as a new species through taxonomic characterization, for which the name Reyranella humidisoli sp. nov. is proposed (type strain = MMS21-HV4-11 T = KCTC 82780 T = LMG 32365T).

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从河边土壤中分离出的 Reyranella sp.
法定人数淬灭是指任何抑制法定人数感应过程的机制。本研究筛选了栖息在河边土壤中的细菌的法定人数淬灭活性,并分离出一株新型革兰氏染色阴性杆状细菌,命名为 MMS21-HV4-11T,该菌株显示出最高水平的法定人数淬灭活性,并对其进行了进一步分析。根据 16S rRNA 基因序列,菌株 MMS21-HV4-11T 与 Reyranella aquatilis seoho-37T 的序列相似度为 98.74%,与 Reyranella soli KIS14-15T 和 Reyranella massiliensis 521T 的序列相似度分别为 97.87%和 97.80%,因此可归属于 Alphaproteobacteria 的 Reyranella 属。在酸性条件下,分解的 N-酰基高丝氨酸内酯在高浓度下得以恢复,这意味着内酯酶和其他酶是造成定量淬灭的原因。基因组分析表明,菌株 MMS21-HV4-11T 有两个内酯酶候选基因和一个酰化酶候选基因,并确认了预期的蛋白质结构。在使用植物病原果胶杆菌(Pectobacterium carotovorum KACC 14888)进行的法定量感应抑制试验中,菌株 MMS21-HV4-11T 显著抑制了软腐病的发生。此外,在菌株 MMS21-HV4-11T 的存在下,铜绿假单胞菌 PA14 的蜂拥运动也受到明显抑制。由于该分离物对这两种假单胞菌都没有直接的抗菌活性,因此这种抑制作用肯定是由法定量淬灭活性引起的。在对所有已知 Reyranella 物种的模式菌株进行的扩展研究中,所有菌株都能降解 N-酰基高丝氨酸内酯(AHLs),因此在属一级显示出了法定人数淬灭潜力。这是首次对雷氏菌的法定熄灭潜能和酶进行研究。此外,通过分类鉴定,MMS21-HV4-11T 可被认定为一个新种,并被命名为 Reyranella humidisoli sp.nov.(模式菌株 = MMS21-HV4-11 T = KCTC 82780 T = LMG 32365T)。
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来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
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