Molecular Insights into the Defense of Dioscorea opposita Cultivar Tiegun Callus Against Pathogenic and Endophytic Fungal Infection Through Transcriptome Analysis.

IF 2.6 2区 农林科学 Q2 PLANT SCIENCES Phytopathology Pub Date : 2024-08-01 Epub Date: 2024-08-05 DOI:10.1094/PHYTO-04-24-0125-R
Chaochuang Li, Lanning Wang, Chenwei Tong, Haibing Li, Zhao Qin, Xiangpeng Zeng, Yingying Chang, Mingjun Li, Qingxiang Yang
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Abstract

Dioscorea opposita cultivar Tiegun is an economically important crop with high nutritional and medicinal value. Plants can activate complex and diverse defense mechanisms after infection by pathogenic fungi. Moreover, endophytic fungi can also trigger the plant immune system to resist pathogen invasion. However, the study of the effects of endophytic fungi on plant infection lags far behind that of pathogenic fungi, and the underlying mechanism is not fully understood. Here, the black spot pathogen Alternaria alternata and the endophytic fungus Penicillium halotolerans of Tiegun were identified and used to infect calli. The results showed that A. alternata could cause more severe membrane lipid peroxidation, whereas P. halotolerans could rapidly increase the activity of the plant antioxidant enzymes superoxide dismutase, peroxidase, and catalase; thus, the degree of damage to the callus caused by P. halotolerans was weaker than that caused by A. alternata. RNA sequencing analysis revealed that various plant defense pathways, such as phenylpropanoid biosynthesis, flavonoid biosynthesis, plant hormone signal transduction, and the mitogen-activated protein kinase signaling pathway, play important roles in triggering the plant immune response during fungal infection. Furthermore, the tryptophan metabolism, betalain biosynthesis, fatty acid degradation, flavonoid biosynthesis, tyrosine metabolism, and isoquinoline alkaloid biosynthesis pathways may accelerate the infection of pathogenic fungi, and the ribosome biogenesis pathway in eukaryotes may retard the damage caused by endophytic fungi. This study lays a foundation for exploring the infection mechanism of yam pathogens and endophytic fungi and provides insight for effective fungal disease control in agriculture.

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通过转录组分析,从分子角度了解薯蓣变种'铁枪'胼胝体对病原真菌和内生真菌感染的防御能力。
Dioscorea opposita cv. 'Tiegun'是一种具有重要经济价值的作物,具有很高的营养和药用价值。植物在受到病原真菌感染后会启动复杂多样的防御机制。此外,内生真菌还能激发植物免疫系统抵抗病原体入侵。然而,对内生真菌对植物感染影响的研究远远落后于对病原真菌影响的研究,而且对其内在机制的了解也不全面。在此,我们鉴定了'铁观音'的黑斑病病原体交替丝核菌和内生真菌卤腐青霉,并用它们感染胼胝体。结果表明,交替孢霉能引起更严重的膜脂过氧化,而卤化青霉能迅速提高植物抗氧化酶超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性;因此,卤化青霉对胼胝体的损伤程度比交替孢霉弱。RNA-seq分析表明,在真菌感染过程中,各种植物防御通路,如苯丙类生物合成、类黄酮生物合成、植物激素信号转导和MAPK信号通路,在触发植物免疫反应中发挥着重要作用。此外,色氨酸代谢、甜菜碱生物合成、脂肪酸降解、类黄酮生物合成、酪氨酸代谢和异喹啉生物碱生物合成途径可能加速病原真菌的感染,真核生物的核糖体生物发生途径可能延缓内生真菌造成的损害。这项研究为探索山药病原菌和内生真菌的感染机制奠定了基础,并为农业领域有效控制真菌病害提供了启示。
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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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