Kilian Lucas Ossetek, Andrea Teresa Müller, Axel Mithöfer
{"title":"Robotic mechanical wounding is sufficient to induce phenylacetaldoxime accumulation in <i>Tococa quadrialata</i>.","authors":"Kilian Lucas Ossetek, Andrea Teresa Müller, Axel Mithöfer","doi":"10.1080/15592324.2024.2360298","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigated the accumulation of phenlyacetaldoxime (PAOx) and PAOx-Glc in <i>Tococa quadrialata</i> leaves in response to herbivore infestation and mechanical wounding. Results show that PAOx levels peaked at 24 h post-infestation, while PAOx-Glc remained present for several days. The accumulation of PAOx began as early as 3 h after herbivory, with PAOx-Glc significantly increased after 6 h. Mechanical wounding induced similar responses in PAOx and PAOx-Glc accumulation as herbivory, suggesting that continuous tissue damage triggers the production of these compounds. Interestingly, SpitWorm-treated leaves showed the highest levels of both PAOx and PAOx-Glc, indicating that herbivore-derived oral secretions (OS) play a role in the induction of these compounds. Additionally, JA-independent PAOx production was found to be associated with tissue damage rather than specific known signaling compounds. Emission of benzyl cyanide and 2-phenylethanol, PAOx-derived plant volatiles, was observed in response to herbivory and SpitWorm treatment providing plant-derived OS, further highlighting the role of herbivore cues in plant defense responses.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2360298"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11141477/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant signaling & behavior","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/15592324.2024.2360298","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/30 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This study investigated the accumulation of phenlyacetaldoxime (PAOx) and PAOx-Glc in Tococa quadrialata leaves in response to herbivore infestation and mechanical wounding. Results show that PAOx levels peaked at 24 h post-infestation, while PAOx-Glc remained present for several days. The accumulation of PAOx began as early as 3 h after herbivory, with PAOx-Glc significantly increased after 6 h. Mechanical wounding induced similar responses in PAOx and PAOx-Glc accumulation as herbivory, suggesting that continuous tissue damage triggers the production of these compounds. Interestingly, SpitWorm-treated leaves showed the highest levels of both PAOx and PAOx-Glc, indicating that herbivore-derived oral secretions (OS) play a role in the induction of these compounds. Additionally, JA-independent PAOx production was found to be associated with tissue damage rather than specific known signaling compounds. Emission of benzyl cyanide and 2-phenylethanol, PAOx-derived plant volatiles, was observed in response to herbivory and SpitWorm treatment providing plant-derived OS, further highlighting the role of herbivore cues in plant defense responses.