Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas

IF 3.7 2区 医学 Q1 PARASITOLOGY International journal for parasitology Pub Date : 2024-09-01 DOI:10.1016/j.ijpara.2024.05.004
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Abstract

Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France.

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挑战主要流行区多棘球蚴分离株之间的系统发育关系。
肺泡棘球蚴病(AE)是一种罕见但严重的疾病,全世界每年有 18,000 多人患病。对多棘球蚴线粒体基因组的完整测序使得研究这种寄生虫的遗传多样性及其时空演变成为可能。我们通过 PCR 扩增了整个线粒体基因组,使用了一个单工反应和两个多工反应来覆盖有丝分裂基因组的 13,738 bp,然后利用 Illumina 技术对扩增子进行了测序。共分析了来自欧洲、亚洲、北极和北美洲的 113 个样本。结果发现了三个主要的单倍群:HG1 聚集了来自阿拉斯加(包括圣劳伦斯岛)、雅库特(俄罗斯)和斯瓦尔巴群岛的样本;HG2 聚集了来自亚洲、北美和欧洲的样本;HG3 则细分为三个微型单倍群。HG3a 包括北美和欧洲的样本,而 HG3b 和 HG3c 只包括欧洲的样本。在法国,HG3a 包括最近在历史流行区以外地区确诊的患者样本。第四个可能的单倍群 HG4 仅有一个来自俄罗斯奥尔洪岛的分离株。多角体细胞有丝分裂基因组的完整测序提高了判别能力,从而突出了四个不同的地理集群,其中一个在法国被分为三个微单倍群。
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来源期刊
CiteScore
8.40
自引率
2.50%
发文量
76
审稿时长
23 days
期刊介绍: International Journal for Parasitology offers authors the option to sponsor nonsubscriber access to their articles on Elsevier electronic publishing platforms. For more information please view our Sponsored Articles page. The International Journal for Parasitology publishes the results of original research in all aspects of basic and applied parasitology, including all the fields covered by its Specialist Editors, and ranging from parasites and host-parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture.
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