Wenpu Wu, Cuichi Yu, Lei Sui, Hui Xu, Jinhua Li, Na Zhou, Lingxin Chen and Zhihua Song
{"title":"Molecularly imprinted polymer-coated silica microbeads for high-performance liquid chromatography†","authors":"Wenpu Wu, Cuichi Yu, Lei Sui, Hui Xu, Jinhua Li, Na Zhou, Lingxin Chen and Zhihua Song","doi":"10.1039/D4AN00239C","DOIUrl":null,"url":null,"abstract":"<p >Molecularly imprinted polymer (MIP)-based chromatographic separation materials, owing to their advantages of unique selectivity, low cost, suitable reproducibility, and acceptable stability, have attracted a great deal of research in different fields. In this investigation, a new type of MIP-coated silica (MIP/SiO<small><sub>2</sub></small>) separation material was developed using sulfamethoxazole as a template; the specific recognition ability of MIP and appropriate physicochemical properties (abundant Si-OH, suitable pore structure, good stability, <em>etc</em>.) of SiO<small><sub>2</sub></small> microbeads were combined. The MIP/SiO<small><sub>2</sub></small> separation materials were characterized carefully. Then, various compounds (such as sulfonamides, ginsenosides, nucleosides, and several pesticides) were used to comprehensively evaluate the chromatographic performances of the MIP/SiO<small><sub>2</sub></small> column. Furthermore, the chromatographic performances of the MIP/SiO<small><sub>2</sub></small> column were compared with those of other separation materials (such as non-imprinted polymer-coated silica, C18/SiO<small><sub>2</sub></small>, and bare silica) packed columns. The resolution value of all measured compounds was more than 1.51. The column efficiencies of 13 510 plates per meter (N m<small><sup>−1</sup></small>) for sulfamethoxazole, 11 600 N m<small><sup>−1</sup></small> for ginsenoside Rd, and 10 510 N m<small><sup>−1</sup></small> for 2′-deoxyadenosine were obtained. The acceptable results verified that the MIP/SiO<small><sub>2</sub></small> column can be applied to separate highly polar drugs such as sulfonamides, ginsenosides, nucleosides, and pesticides.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analyst","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/an/d4an00239c","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Molecularly imprinted polymer (MIP)-based chromatographic separation materials, owing to their advantages of unique selectivity, low cost, suitable reproducibility, and acceptable stability, have attracted a great deal of research in different fields. In this investigation, a new type of MIP-coated silica (MIP/SiO2) separation material was developed using sulfamethoxazole as a template; the specific recognition ability of MIP and appropriate physicochemical properties (abundant Si-OH, suitable pore structure, good stability, etc.) of SiO2 microbeads were combined. The MIP/SiO2 separation materials were characterized carefully. Then, various compounds (such as sulfonamides, ginsenosides, nucleosides, and several pesticides) were used to comprehensively evaluate the chromatographic performances of the MIP/SiO2 column. Furthermore, the chromatographic performances of the MIP/SiO2 column were compared with those of other separation materials (such as non-imprinted polymer-coated silica, C18/SiO2, and bare silica) packed columns. The resolution value of all measured compounds was more than 1.51. The column efficiencies of 13 510 plates per meter (N m−1) for sulfamethoxazole, 11 600 N m−1 for ginsenoside Rd, and 10 510 N m−1 for 2′-deoxyadenosine were obtained. The acceptable results verified that the MIP/SiO2 column can be applied to separate highly polar drugs such as sulfonamides, ginsenosides, nucleosides, and pesticides.