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DL-assisted Self-volume-calibrating Colorimetric PAAHM Sensors for Water Surveillance dl辅助自容量校准比色PAAHM水监测传感器

IF 4.2 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-07 DOI: 10.1039/d5an01216c

Miaorong Lin, Jihan Qu, Ting Xiao, Tingting Yang, Jiahui Chen, Jianxin Meng, Fengyu Li

This work introduces a colorimetric sensing platform based on sodium polyacrylate hydrogel microspheres (PAAHM) integrated with a deep learning-assisted self-volume calibration strategy for the efficient and quantitative detection of NH4+, PO43-, and Fe2+ in water. The PAAHM are uniformly loaded with colorimetric indicators through a simple immersion process, significantly simplifying sensor fabrication and enabling rapid, consistent, large-scale production. Moreover, the PAAHM exhibits dual responsiveness-both colorimetric and volumetric-allowing for the simultaneous detection of analyte concentration changes and sample volume fluctuations. To address the limitations of conventional RGB analysis, a self-volume calibration method was implemented in conjunction with a Convolutional Neural Network (CNN) to automatically extract and model both colour and morphological features from sensor images. The results demonstrate that the CNN model achieves an R2 correlation coefficient of 0.999 for concentration prediction and 100% classification accuracy. This approach offers a convenient, low-cost, and highly accurate solution for on-site environmental monitoring, presenting significant potential for broad application.

{"title":"DL-assisted Self-volume-calibrating Colorimetric PAAHM Sensors for Water Surveillance","authors":"Miaorong Lin, Jihan Qu, Ting Xiao, Tingting Yang, Jiahui Chen, Jianxin Meng, Fengyu Li","doi":"10.1039/d5an01216c","DOIUrl":"https://doi.org/10.1039/d5an01216c","url":null,"abstract":"This work introduces a colorimetric sensing platform based on sodium polyacrylate hydrogel microspheres (PAAHM) integrated with a deep learning-assisted self-volume calibration strategy for the efficient and quantitative detection of NH4+, PO43-, and Fe2+ in water. The PAAHM are uniformly loaded with colorimetric indicators through a simple immersion process, significantly simplifying sensor fabrication and enabling rapid, consistent, large-scale production. Moreover, the PAAHM exhibits dual responsiveness-both colorimetric and volumetric-allowing for the simultaneous detection of analyte concentration changes and sample volume fluctuations. To address the limitations of conventional RGB analysis, a self-volume calibration method was implemented in conjunction with a Convolutional Neural Network (CNN) to automatically extract and model both colour and morphological features from sensor images. The results demonstrate that the CNN model achieves an R2 correlation coefficient of 0.999 for concentration prediction and 100% classification accuracy. This approach offers a convenient, low-cost, and highly accurate solution for on-site environmental monitoring, presenting significant potential for broad application.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"94 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal Desorption–Photoionization Ion Mobility Spectrometry for Rapid Analysis of Tobacco Flavorings and Flavored Filters 热解吸-光电离离子迁移光谱法快速分析烟草香料和调味滤嘴

IF 4.2 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-07 DOI: 10.1039/d5an01174d

Yajin He, Yating Yao, Yue Zhang, Yawen Guo, Huaiwen Cang, Weiguo Wang, Jinghua Li, Bing Wang, Shuang Wang, Wuduo Zhao

This study developed a rapid thermal desorption sampling device and integrated it with photoionization ion mobility spectrometry, establishing a system for rapid characterization of liquid flavor components and quantitative detection of specific flavor compounds in tobacco filter rods. Its core innovation lies in the construction of a detection platform capable of analyzing both liquid and solid matrices: only 2 μL of sample is needed to evaluate the quality of liquid flavorings within 20 seconds, while under conditions simulating the actual flavoring process, the target components in solid filter rods can be quantitatively analyzed within 15 minutes. Using WS-23 as a model analyte, the method established a linear range of 1–20 mg/mL (R2=0.99), with limits of detection (LOD) and quantitation (LOQ) of 0.229 mg/mL and 0.765 mg/mL, respectively. Relative standard deviations (RSDs) ranged from 1.73% to 6.17%. Applying this method to the rapid detection of WS-23 in commercially available menthol-flavored cigarette filters not only meets analytical requirements but also effectively distinguishes the differences in flavor additive content among different brands. The integrated system and analysis strategy developed in this study have the advantages of rapid response, simple operation, and high sensitivity, meeting industrial quantitative requirements. Furthermore, it can achieve full-process coverage from the quality control of flavor raw materials to the monitoring of the total release of flavor components in filter rods, providing a reliable technical means for the precise control of the flavor quality of filter rods in industrial production.

{"title":"Thermal Desorption–Photoionization Ion Mobility Spectrometry for Rapid Analysis of Tobacco Flavorings and Flavored Filters","authors":"Yajin He, Yating Yao, Yue Zhang, Yawen Guo, Huaiwen Cang, Weiguo Wang, Jinghua Li, Bing Wang, Shuang Wang, Wuduo Zhao","doi":"10.1039/d5an01174d","DOIUrl":"https://doi.org/10.1039/d5an01174d","url":null,"abstract":"This study developed a rapid thermal desorption sampling device and integrated it with photoionization ion mobility spectrometry, establishing a system for rapid characterization of liquid flavor components and quantitative detection of specific flavor compounds in tobacco filter rods. Its core innovation lies in the construction of a detection platform capable of analyzing both liquid and solid matrices: only 2 μL of sample is needed to evaluate the quality of liquid flavorings within 20 seconds, while under conditions simulating the actual flavoring process, the target components in solid filter rods can be quantitatively analyzed within 15 minutes. Using WS-23 as a model analyte, the method established a linear range of 1–20 mg/mL (R2=0.99), with limits of detection (LOD) and quantitation (LOQ) of 0.229 mg/mL and 0.765 mg/mL, respectively. Relative standard deviations (RSDs) ranged from 1.73% to 6.17%. Applying this method to the rapid detection of WS-23 in commercially available menthol-flavored cigarette filters not only meets analytical requirements but also effectively distinguishes the differences in flavor additive content among different brands. The integrated system and analysis strategy developed in this study have the advantages of rapid response, simple operation, and high sensitivity, meeting industrial quantitative requirements. Furthermore, it can achieve full-process coverage from the quality control of flavor raw materials to the monitoring of the total release of flavor components in filter rods, providing a reliable technical means for the precise control of the flavor quality of filter rods in industrial production.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"51 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances of Microneedle-Based Electrochemical Biosensors for Monitoring Biomarkers in Interstitial Fluid 微针电化学生物传感器监测组织间质液中生物标志物的研究进展

IF 4.2 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-06 DOI: 10.1039/d5an01250c

Renqiang Yuan, Xiaoxin Ma, Guda Zou, Guohuang Zhou, Wei Li, Jia Liu, Yixin Xu, Shao Su, Lianhui Wang

Microneedle (MN)-based electrochemical biosensors have emerged as a revolutionary technology for minimally invasive diagnostic applications, particularly point-of-care (POC) monitoring of biomarkers in interstitial fluid (ISF). Featuring painless skin penetration, rapid electrochemical response, high sensitivity, and seamless integration with portable/wireless devices, MN-based electrochemical biosensors offer distinct advantages over conventional invasive or lab-based assays. Therefore, this review comprehensively summarizes the recent advances in MN-based electrochemical biosensors. It begins by introducing the design strategies and unique advantages of different microneedle platforms for efficient ISF sampling. Subsequently, the review elaborates on the construction of MN-based electrochemical biosensors. Furthermore, the application progress of these biosensors for monitoring a wide range of biomarkers, including metabolites, hormones, electrolytes, nucleic acids and proteins, is systematically highlighted. Finally, the current challenges and future perspectives in this rapidly evolving field are discussed, outlining the development path toward next-generation MN-based electrochemical diagnostic devices.

{"title":"Recent Advances of Microneedle-Based Electrochemical Biosensors for Monitoring Biomarkers in Interstitial Fluid","authors":"Renqiang Yuan, Xiaoxin Ma, Guda Zou, Guohuang Zhou, Wei Li, Jia Liu, Yixin Xu, Shao Su, Lianhui Wang","doi":"10.1039/d5an01250c","DOIUrl":"https://doi.org/10.1039/d5an01250c","url":null,"abstract":"Microneedle (MN)-based electrochemical biosensors have emerged as a revolutionary technology for minimally invasive diagnostic applications, particularly point-of-care (POC) monitoring of biomarkers in interstitial fluid (ISF). Featuring painless skin penetration, rapid electrochemical response, high sensitivity, and seamless integration with portable/wireless devices, MN-based electrochemical biosensors offer distinct advantages over conventional invasive or lab-based assays. Therefore, this review comprehensively summarizes the recent advances in MN-based electrochemical biosensors. It begins by introducing the design strategies and unique advantages of different microneedle platforms for efficient ISF sampling. Subsequently, the review elaborates on the construction of MN-based electrochemical biosensors. Furthermore, the application progress of these biosensors for monitoring a wide range of biomarkers, including metabolites, hormones, electrolytes, nucleic acids and proteins, is systematically highlighted. Finally, the current challenges and future perspectives in this rapidly evolving field are discussed, outlining the development path toward next-generation MN-based electrochemical diagnostic devices.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"134 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Initial testing procedure based on microextraction followed by LC-HRMS analysis to determine 107 xenobiotics and their metabolites in serum/plasma 最初的检测程序是基于微萃取，然后是LC-HRMS分析，以确定血清/血浆中的107种外源性药物及其代谢物

IF 4.2 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-06 DOI: 10.1039/d5an01329a

Monica Mazzarino, Koen Deventer, Laurie De Wilde, Kris Roels, Peter Van Eenoo

A simple and sensitive microextraction protocol based on the use of unmodified cellulose was developed for the simultaneous extraction of 107 prohibited compounds and their metabolites from 20 µL of serum and plasma. Sample preparation consisted of spotting 20 µL of serum/plasma onto a cellulose card, followed by extraction of the analytes with 500 µL of a methanol/acetonitrile (1 : 1, v/v) mixture for 20 min. The extracts were analyzed by liquid chromatography coupled to high-resolution mass spectrometry. The entire workflow was validated in terms of selectivity (no interferences were detected at the retention times of the target analytes), sensitivity (limits of detection in the range of 0.08–7.50 ng mL⁻¹) carry-over (no signals in the negative sample injected after the positive sample at high concentration), matrix effect (10–28%), extraction yield (42–89%), and extract stability (the analytes were stable for at least 72 h in the autosampler at 10 °C). The method was successfully applied to the analysis of samples containing the compounds at low nanogram per milliliter range, demonstrating its effectiveness for doping control purposes. Stability studies showed that the compounds were stable for at least 3 months at −20 and 4 °C in serum and plasma samples. In contrast, at 22 °C several thiazide-based compounds were completely degraded after 4 weeks; FG2216 was no longer detectable after 7 weeks; S6 and RAD140 were no longer detectable after 9 weeks, whereas trenbolone was completely degraded after 14 weeks. The other compounds were still visible for the entire study period, with variations in the range of 37–56%.

{"title":"Initial testing procedure based on microextraction followed by LC-HRMS analysis to determine 107 xenobiotics and their metabolites in serum/plasma","authors":"Monica Mazzarino, Koen Deventer, Laurie De Wilde, Kris Roels, Peter Van Eenoo","doi":"10.1039/d5an01329a","DOIUrl":"https://doi.org/10.1039/d5an01329a","url":null,"abstract":"A simple and sensitive microextraction protocol based on the use of unmodified cellulose was developed for the simultaneous extraction of 107 prohibited compounds and their metabolites from 20 µL of serum and plasma. Sample preparation consisted of spotting 20 µL of serum/plasma onto a cellulose card, followed by extraction of the analytes with 500 µL of a methanol/acetonitrile (1 : 1, v/v) mixture for 20 min. The extracts were analyzed by liquid chromatography coupled to high-resolution mass spectrometry. The entire workflow was validated in terms of selectivity (no interferences were detected at the retention times of the target analytes), sensitivity (limits of detection in the range of 0.08–7.50 ng mL−1) carry-over (no signals in the negative sample injected after the positive sample at high concentration), matrix effect (10–28%), extraction yield (42–89%), and extract stability (the analytes were stable for at least 72 h in the autosampler at 10 °C). The method was successfully applied to the analysis of samples containing the compounds at low nanogram per milliliter range, demonstrating its effectiveness for doping control purposes. Stability studies showed that the compounds were stable for at least 3 months at −20 and 4 °C in serum and plasma samples. In contrast, at 22 °C several thiazide-based compounds were completely degraded after 4 weeks; FG2216 was no longer detectable after 7 weeks; S6 and RAD140 were no longer detectable after 9 weeks, whereas trenbolone was completely degraded after 14 weeks. The other compounds were still visible for the entire study period, with variations in the range of 37–56%.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"15 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing microscopic calcification deposits on acrylic intraocular lenses. 亚克力人工晶体显微钙化沉积特征。

IF 3.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-06 DOI: 10.1039/d5an00999e

Cassandra L Ward, S Sameera Perera, Zhi Mei, Bing X Ross, Manisha De Alwis Goonatilleke, Mahdi Ayoubi, Yao Xiao, Andrew P Ault, Judy A Westrick, Thomas H Linz, Xihui Lin

Intraocular lenses (IOLs) are implanted into the eyes during cataract surgery to improve vision. A rare complication following IOL implantation is the formation of crystallized deposits on the lenses, which significantly impair vision, necessitating subsequent surgical IOL exchange. Preventing these deposits from forming is critical, but this requires definitive molecular assignments of the crystals and knowledge of their mechanism of formation. Determining this information presents a significant analytical challenge due to the low numbers of crystals and curvature of the IOLs that limit use of conventional methods. Here, we report the development of multiple complementary analytical methods to characterize IOLs explanted from human patients and attain insight into the chemical identity and mechanism of the deposits. Initial elemental analyses using energy-dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) revealed that IOL crystals contained calcium, phosphorus, and sodium and provided quantitative ratios between elements. Subsequent Raman spectroscopy identified carbonate in the crystals as well. An innovative single-crystal X-ray diffraction (XRD) method with integrated Rietveld analysis was then developed, which conclusively determined that IOL crystals were composed of substituted hydroxyapatite. Collectively, the data obtained from EDS, XPS, XRD, and Raman indicate that IOL deposits are composed of crystalline Ca₉Na(PO₄)₅(CO₃)(OH)₂ layered with amorphous calcium phosphate. This structure is similar to bone, suggesting that a similar ossification mechanism is followed. The robust analytical methods developed herein provide the most comprehensive characterization of IOL crystals to date and signify that the microenvironment of the eye is conducive to bone mineralization pathways that induce crystal formation on IOLs.

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引用次数: 0

Magnetic molecularly imprinted polymers-assisted target enrichment for fluorescence detection of chloramphenicol in water 磁性分子印迹聚合物辅助靶富集用于水中氯霉素的荧光检测

IF 4.2 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-05 DOI: 10.1039/d6an00075d

Zan Long, Jiajun Guo, Xiaofeng Liu, Caicheng Long, Peng Zhang, Bo Feng, Taiping Qing

The abuse of antibiotics has induced various environmental problems and poses a threat to human health and life. Fluorescence analysis method has received extensive attention in the fields of antibiotic detection due to its high sensitivity and specificity. However, traditional fluorescence methods is confronted with the challenges of background interference and sample complexity, and there is an urgent need to develop efficient detection methods. Herein, an efficient sample separation and enrichment method based on magnetic molecularly imprinted polymers (MMIPs) was introduced into the fluorescence biosensor for anti-interference detection of antibiotics in water. As the proof-of-concept of our approach, chloramphenicol (CAP) was chosen as a model antibiotic to be investigated. The MMIPs were synthesized using surface molecular imprinting technology on Fe3O4 nanoparticles, achieving high selectivity and adsorption capacity (49.6 mg/g). More importantly, the MMIPs-assisted fluorescent probe had good anti-interference ability, while the ordinary fluorescent probe was easily interfered by coexisting substances (such as humic acid) in the water, resulting in poor detection performance. When detecting CAP in real water samples, the MMIPs-assisted biosensor still showed good detection performance and anti-interference ability. These results demonstrated the potential of MMIPs as an efficient, cost-effective tool for CAP extraction in environmental samples, offering a promising approach for environmental monitoring of antibiotic contamination.

抗生素的滥用引发了各种环境问题，对人类的健康和生命构成了威胁。荧光分析法以其高灵敏度和特异性在抗生素检测领域受到广泛关注。然而，传统的荧光检测方法面临着背景干扰和样品复杂性的挑战，迫切需要开发高效的检测方法。本文将基于磁性分子印迹聚合物（MMIPs）的高效样品分离富集方法引入到荧光生物传感器中，用于水中抗生素的抗干扰检测。作为我们方法的概念验证，氯霉素（CAP）被选为模型抗生素进行研究。采用表面分子印迹技术在Fe3O4纳米颗粒上合成了MMIPs，具有较高的选择性和吸附量（49.6 mg/g）。更重要的是，mmips辅助荧光探针具有良好的抗干扰能力，而普通荧光探针容易受到水中共存物质（如腐植酸）的干扰，导致检测性能较差。在实际水样中检测CAP时，mmips辅助生物传感器仍然表现出良好的检测性能和抗干扰能力。这些结果证明了MMIPs作为一种高效、经济的环境样品CAP提取工具的潜力，为抗生素污染的环境监测提供了一种有前途的方法。

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引用次数: 0

Versatile polymeric membrane ion-selective electrodes based on cellulose triacetate 基于三醋酸纤维素的多功能聚合物膜离子选择电极

IF 4.2 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-04 DOI: 10.1039/d5an01364j

Lu Liu, Shusheng Liu, Rongning Liang, Wei Qin

Nowadays, polymeric membrane ion-selective electrodes (ISEs) based on poly(vinyl chloride) (PVC) matrix have been widely used in in clinical diagnosis and environmental monitoring. However, chemical inert of PVC may limit sensor applications in some scenarios, such as surface modification and construction of potentiometric biosensors through surface grafting of receptors. In this work, cellulose triacetate (CTA) is employed as membrane matrix to prepare polymeric membrane ISEs. The versatile properties of the proposed CTA-based potentiometric sensors in terms of biocompatibility, biodegradability and easy chemical modification are illustrated. As a proof-of-concept experiment, the polymeric membrane Ca2+-ISEs using CTA as the membrane matrix are fabricated. The electrode has a linear range of 1.0 ×10⁻5–1.0 ×10⁻2 M with a Nernstian slope of 29.19 ± 0.97 mV dec⁻¹. A surface hydrophilic CTA-based Ca2+-ISE membrane can be simply obtained by alkaline hydrolysis of the electrode. The hydrolyzed surface of the obtained membrane can further be activated with carbonyldiimidazole (CDI) for the direct immobilization of the functionalized agent, polysaccharide chitosan, on the surface of the membranes. In addition, an enzyme, butyrylcholinesterase, can also be immobilized onto the ISE surface through a similar CDI reaction, which providing a great potential for fabrication of new potentiometric biosensors. It will be shown that CTA can be used as a powerful membrane matrix alternative for the classical PVC matrix for fabrication of polymeric membrane optical and electrochemical sensors in clinical applications.

目前，以聚氯乙烯（PVC）为基体的聚合物膜离子选择电极（ISEs）已广泛应用于临床诊断和环境监测。然而，PVC的化学惰性可能会限制传感器在某些情况下的应用，例如通过表面接枝受体进行表面改性和构建电位生物传感器。本研究以三乙酸纤维素（CTA）为膜基质，制备聚合膜。本文从生物相容性、生物可降解性和易化学修饰等方面阐述了所提出的基于cta的电位传感器的多种特性。作为一个概念验证实验，制备了以CTA为膜基质的Ca2+-ISEs聚合物膜。该电极的线性范围为1.0 ×10 - 1.0 ×10 - 2 M，能量斜率为29.19±0.97 mV dec - 1。通过对电极进行碱性水解，可以得到表面亲水的cta基Ca2+-ISE膜。得到的水解膜表面可以进一步用羰基二咪唑（CDI）活化，将功能化剂多糖壳聚糖直接固定在膜表面。此外，一种酶，丁基胆碱酯酶，也可以通过类似的CDI反应固定在ISE表面，这为制造新的电位生物传感器提供了巨大的潜力。研究结果表明，CTA可以作为一种强大的膜基质替代传统的PVC基质，在临床应用中用于制造聚合物膜光学和电化学传感器。

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引用次数: 0

Correction: Light-activated nanocomposite thin sheet for high throughput contactless biomolecular delivery into hard-to-transfect cells 校正：光激活纳米复合材料薄片，用于高通量非接触生物分子递送到难以转染的细胞中

IF 4.2 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-04 DOI: 10.1039/d5an90082d

Hima Harshan Padma, Donia Dominic, Kavitha Illath, Srabani Kar, Tuhin Subhra Santra

Correction for ‘Light-activated nanocomposite thin sheet for high throughput contactless biomolecular delivery into hard-to-transfect cells’ by Hima Harshan Padma et al., Analyst, 2025, 150, 860–876, https://doi.org/10.1039/D4AN01331J.

修正Hima Harshan Padma等人的“用于高通量非接触式生物分子递送到难以转染细胞的光活化纳米复合薄片”，Analyst, 2025, 150, 860-876, https://doi.org/10.1039/D4AN01331J。

引用次数: 0

Resolving the chemical space: a legacy of recording separation science and innovation in Analyst. 解决化学空间：在分析记录分离科学和创新的遗产。

IF 3.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-03 DOI: 10.1039/d5an90086g

Leon P Barron, Brett Paull, Lihua Zhang

引用次数: 0

One-Pot Ligation-PCR for Universal RNA Biomarker Detection 通用RNA生物标志物的单罐连接pcr检测

IF 4.2 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst

Pub Date : 2026-02-03 DOI: 10.1039/d5an01203a

Jinding Liu, Zhixin Xie, Nini Li, Jiajia Li, Minghua Zhang, Yongqiang Cheng, Jiangyan Zhang

In the detection of RNA biomarkers, conventional PCR-based workflows such as reverse transcription-PCR (RT-PCR) and ligation-PCR each exhibit inherent limitations. RT-PCR requires cDNA synthesis and often struggles with targets of high sequence similarity or atypical length and structure. Ligation-PCR improves sequence discrimination by enzymatically joining adjacent probes only when perfectly matched, yet conventional two-step formats—where ligation and amplification are performed separately—introduce workflow complexity, increased handling, and contamination risk. To overcome these challenges, we developed a glyoxal-assisted one-pot ligation-PCR assay that integrates probe ligation and PCR amplification within a single closed-tube system. The method employs thermally responsive glyoxal-caged primers that remain inactive during the ligation phase and are gradually activated during PCR cycling, thereby preventing premature extension and minimizing nonspecific amplification. Validated primarily on mRNA splice variants, the assay achieved sensitivity comparable to conventional two-step ligation-PCR while providing markedly improved discrimination among closely related splice isoforms. Additional experiments demonstrate the feasibility of extending this strategy to microRNA detection. This streamlined one-tube strategy simplifies operation, reduces contamination risk, and establishes a robust and efficient one-pot ligation-PCR framework that is readily adaptable to different RNA targets for precise RNA biomarker detection.

在RNA生物标志物的检测中，传统的基于pcr的工作流程，如逆转录- pcr （RT-PCR）和连接- pcr，都存在固有的局限性。RT-PCR需要cDNA合成，并且经常与高序列相似性或非典型长度和结构的目标作斗争。只有在完全匹配的情况下，连接- pcr才能通过酶连接相邻探针来改善序列识别，然而传统的两步格式（连接和扩增分开进行）引入了工作流程的复杂性，增加了处理和污染风险。为了克服这些挑战，我们开发了一种乙二醛辅助的单罐结扎PCR检测方法，该方法将探针结扎和PCR扩增整合在一个单一的闭管系统中。该方法采用热响应型乙二醛笼引物，在连接阶段保持无活性，在PCR循环过程中逐渐激活，从而防止过早延伸并最大限度地减少非特异性扩增。主要在mRNA剪接变体上进行验证，该方法的灵敏度与传统的两步连接- pcr相当，同时在密切相关的剪接异构体之间提供了显着改善的区分。其他实验证明了将该策略扩展到microRNA检测的可行性。这种流线型的单管策略简化了操作，降低了污染风险，并建立了一个强大而高效的单罐连接- pcr框架，易于适应不同的RNA靶标，用于精确的RNA生物标志物检测。

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