Sanna Johanna Viitanen, Sabrine Moya Gehani, Anni Maria Tilamaa, Minna Marjaana Rajamäki, Ruud Anthonius Wilhelmus Veldhuizen
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引用次数: 0
Abstract
Background: A syndrome of acute non-cardiogenic pulmonary edema associated with hunting is prevalent in the drever breed, but etiology of this syndrome is currently unknown. Alveolar surfactant has a critical role in preventing alveolar collapse and edema formation. The aim of this study was to investigate, whether the predisposition to hunting associated pulmonary edema in drever dogs is associated with impaired biophysical properties of alveolar surfactant. Seven privately owned drever dogs with recurrent hunting associated pulmonary edema and seven healthy control dogs of other breeds were included in the study. All affected dogs underwent thorough clinical examinations including echocardiography, laryngeal evaluation, bronchoscopy, and bronchoalveolar lavage (BAL) as well as head, neck and thoracic computed tomography imaging to rule out other cardiorespiratory diseases potentially causing the clinical signs. Alveolar surfactant was isolated from frozen, cell-free supernatants of BAL fluid and biophysical analysis of the samples was completed using a constrained sessile drop surfactometer. Statistical comparisons over consecutive compression expansion cycles were performed using repeated measures ANOVA and comparisons of single values between groups were analyzed using T-test.
Results: There were no significant differences between groups in any of the biophysical outcomes of surfactant analysis. The critical function of surfactant, reducing the surface tension to low values upon compression, was similar between healthy dogs and affected drevers.
Conclusions: The etiology of hunting associated pulmonary edema in drever dogs is not due to an underlying surfactant dysfunction.
背景:与狩猎有关的急性非心源性肺水肿综合征在德瑞弗犬种中很普遍,但目前病因不明。肺泡表面活性物质在防止肺泡塌陷和水肿形成方面起着至关重要的作用。本研究的目的是调查德瑞弗犬狩猎相关肺水肿的易感性是否与肺泡表面活性物质的生物物理特性受损有关。研究对象包括七只反复出现狩猎相关性肺水肿的私人饲养的德瑞弗犬和七只其他品种的健康对照犬。所有患犬均接受了全面的临床检查,包括超声心动图、喉部评估、支气管镜检查、支气管肺泡灌洗(BAL)以及头颈部和胸部计算机断层扫描成像,以排除可能导致临床症状的其他心肺疾病。肺泡表面活性物质是从冷冻的无细胞BAL液上清液中分离出来的,样本的生物物理分析是使用受限无柄液滴表面活性仪完成的。连续压缩膨胀周期的统计比较采用重复测量方差分析,组间单个值的比较采用 T 检验:结果:在表面活性物质分析的生物物理结果方面,各组之间没有明显差异。表面活性物质的关键功能是在压缩时将表面张力降低到低值,健康犬和患病 drevers 的这一功能相似:德瑞弗犬狩猎相关性肺水肿的病因不是潜在的表面活性物质功能障碍。
期刊介绍:
Acta Veterinaria Scandinavica is an open access journal encompassing all aspects of veterinary research and medicine of domestic and wild animals.