Chlamydia trachomatis upregulates lncRNA CYTOR to mediate autophagy through miR-206/MAPK1 axis.

IF 2.7 4区医学 Q3 IMMUNOLOGY Pathogens and disease Pub Date : 2024-02-07 DOI:10.1093/femspd/ftae011

Shan Cheng, Yi Liu, Bei He, Jinrong Zhang, Yewei Yang, Xinglv Wang, Zhongyu Li

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Abstract

Chlamydia trachomatis infection can be regulated by autophagy-related genes. LncRNA CYTOR has been proven to be involved in autophagy. In this research, we investigated the role of CYTOR in autophagy induced by C. trachomatis and the potential mechanisms. After C. trachomatis infection, CYTOR and MAPK1 were up-regulated and miR-206 was down-regulated, meanwhile, the autophagy-related protein Beclin1 and LC3-Ⅱ/LC3-Ⅰ ratio were increased. Interference with CYTOR or overexpression with miR-206 downregulated the autophagy-related protein Beclin1 and the number of autophagic spots LC3, decreased the protein ratio of LC3-II/LC3-I, and upregulated the expression of P62 protein. The luciferase reporter assay confirmed that CYTOR acted as a sponge for miR-206 to target MAPK1. In addition, CYTOR promoted autophagy induced by C. trachomatis infection through the MAPK1/ERK signaling pathway activation. Taken together, we have identified a novel molecular mechanism that the CYTOR/miR-206/MAPK1 axis was involved in the regulation of autophagy in C. trachomatis infection. This work provides an experimental basis for elucidating the pathogenesis of C. trachomatis for the treatment, prevention and control of related infectious diseases.

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沙眼衣原体通过 miR-206/MAPK1 轴上调 lncRNA CYTOR 以介导自噬。

沙眼衣原体感染可受自噬相关基因的调控。LncRNA CYTOR已被证实参与自噬。本研究探讨了CYTOR在沙眼衣原体诱导的自噬中的作用及其潜在机制。沙眼衣原体感染后，CYTOR和MAPK1上调，miR-206下调，同时自噬相关蛋白Beclin1和LC3-Ⅱ/LC3-Ⅰ比值升高。干扰CYTOR或过表达miR-206可下调自噬相关蛋白Beclin1和自噬点LC3的数量，降低LC3-Ⅱ/LC3-Ⅰ的蛋白比值，上调P62蛋白的表达。荧光素酶报告实验证实，CYTOR 是 miR-206 靶向 MAPK1 的海绵。此外，CYTOR 通过激活 MAPK1/ERK 信号通路促进沙眼衣原体感染诱导的自噬。综上所述，我们发现了一种新的分子机制，即 CYTOR/miR-206/MAPK1 轴参与了沙眼衣原体感染中自噬的调控。这项工作为阐明沙眼衣原体的发病机制，治疗、预防和控制相关传染病提供了实验依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathogens and disease IMMUNOLOGY-INFECTIOUS DISEASES

CiteScore

7.40

自引率

3.00%

发文量

期刊介绍： Pathogens and Disease publishes outstanding primary research on hypothesis- and discovery-driven studies on pathogens, host-pathogen interactions, host response to infection and their molecular and cellular correlates. It covers all pathogens – eukaryotes, prokaryotes, and viruses – and includes zoonotic pathogens and experimental translational applications.