Toward biomanufacturing of next-generation bacterial nanocellulose (BNC)-based materials with tailored properties: A review on genetic engineering approaches

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology advances Pub Date : 2024-05-31 DOI:10.1016/j.biotechadv.2024.108390
Dariela Núñez , Patricio Oyarzún , Sebastián González , Irene Martínez
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Abstract

Bacterial nanocellulose (BNC) is a biopolymer that is drawing significant attention for a wide range of applications thanks to its unique structure and excellent properties, such as high purity, mechanical strength, high water holding capacity and biocompatibility. Nevertheless, the biomanufacturing of BNC is hindered due to its low yield, the instability of microbial strains and cost limitations that prevent it from being mass-produced on a large scale. Various approaches have been developed to address these problems by genetically modifying strains and to produce BNC-based biomaterials with added value. These works are summarized and discussed in the present article, which include the overexpression and knockout of genes related and not related with the nanocellulose biosynthetic operon, the application of synthetic biology approaches and CRISPR/Cas techniques to modulate BNC biosynthesis. Further discussion is provided on functionalized BNC-based biomaterials with tailored properties that are incorporated in-vivo during its biosynthesis using genetically modified strains either in single or co-culture systems (in-vivo manufacturing). This novel strategy holds potential to open the road toward cost-effective production processes and to find novel applications in a variety of technology and industrial fields.

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实现具有定制特性的下一代细菌纳米纤维素(BNC)材料的生物制造:基因工程方法综述。
细菌纳米纤维素(BNC)是一种生物聚合物,因其独特的结构和优异的性能(如高纯度、机械强度、高保水能力和生物相容性),在广泛的应用领域备受关注。然而,由于产量低、微生物菌株不稳定以及成本限制,BNC 的生物制造受到阻碍,无法大规模生产。为了解决这些问题,人们开发了各种方法,通过对菌株进行基因改造,生产出具有附加值的基于 BNC 的生物材料。本文对这些工作进行了总结和讨论,其中包括过表达和敲除与纳米纤维素生物合成操作子有关和无关的基因、应用合成生物学方法和 CRISPR/Cas 技术来调节 BNC 的生物合成。报告还进一步讨论了基于 BNC 的功能化生物材料,这些材料具有量身定制的特性,在生物合成过程中,利用单培养系统或共培养系统(体内制造)中的转基因菌株将其纳入体内。这种新颖的战略有望开辟具有成本效益的生产工艺之路,并在各种技术和工业领域找到新的应用。
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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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