Gypenoside A Protects Human Myocardial Cells from Ischemia/Reperfusion Injury via the circ_0010729/miR-370-3p/RUNX1 Axis

IF 2.3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry (Moscow) Pub Date : 2024-05-31 DOI:10.1134/s000629792405016x

Hailiang Ma, Yuanben Lu, Dewen Zhu, Zhenhua Jiang, FanZhi Zhang, Jun Peng, Li Wang

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Abstract

Ischemia/reperfusion (I/R) injury is one of the major causes of cardiovascular disease. Gypenoside A (GP), the main active component of Gynostemma pentaphyllum, alleviates myocardial I/R injury. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in the I/R injury. We explored the protective effect of GP on human cardiomyocytes (HCMs) via the circ_0010729/miR-370-3p/RUNX1 axis. Overexpression of circ_0010729 abolished the effects of GP on HMC, such as suppression of apoptosis and increase in cell viability and proliferation. Overexpression of miR-370-3p reversed the effect of circ_0010729 overexpression, resulting in the stimulation of HMC viability and proliferation and inhibition of apoptosis. The knockdown of miR-370-3p suppressed the effects of GP in HCMs. RUNX1 silencing counteracted the effect of miR-370-3p knockdown and maintained GP-induced suppression of apoptosis and stimulation of HMC viability and proliferation. The levels of RUNX1 mRNA and protein were reduced in cells expressing miR-370-3p. In conclusion, this study confirmed that GP alleviated the I/R injury of myocardial cell via the circ_0010729/miR-370-3p/RUNX1 axis.

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Gypenoside A 通过 circ_0010729/miR-370-3p/RUNX1 轴保护人类心肌细胞免受缺血再灌注损伤

摘要缺血/再灌注（I/R）损伤是心血管疾病的主要原因之一。绞股蓝的主要活性成分绞股蓝甙 A（GP）可减轻心肌 I/R 损伤。环状 RNA（circRNA）和微 RNA（miRNA）参与了 I/R 损伤。我们探讨了 GP 通过 circ_0010729/miR-370-3p/RUNX1 轴对人类心肌细胞（HCMs）的保护作用。过量表达 circ_0010729 可消除 GP 对 HCM 的影响，如抑制细胞凋亡、提高细胞活力和增殖。miR-370-3p的过表达逆转了circ_0010729过表达的效应，从而刺激了HMC的活力和增殖，抑制了细胞凋亡。敲除 miR-370-3p 可抑制 GP 对 HCMs 的影响。RUNX1 沉默抵消了 miR-370-3p 敲除的作用，维持了 GP 诱导的抑制细胞凋亡和刺激 HMC 活力与增殖的作用。在表达 miR-370-3p 的细胞中，RUNX1 mRNA 和蛋白水平均有所下降。总之，本研究证实了GP通过circ_0010729/miR-370-3p/RUNX1轴减轻了心肌细胞的I/R损伤。

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来源期刊

Biochemistry (Moscow) 生物-生化与分子生物学

CiteScore

4.70

自引率

3.60%

发文量

139

审稿时长

2 months

期刊介绍： Biochemistry (Moscow) is the journal that includes research papers in all fields of biochemistry as well as biochemical aspects of molecular biology, bioorganic chemistry, microbiology, immunology, physiology, and biomedical sciences. Coverage also extends to new experimental methods in biochemistry, theoretical contributions of biochemical importance, reviews of contemporary biochemical topics, and mini-reviews (News in Biochemistry).