Familiarize Students with Direct MS Analysis Methods: Localization of Components in Citrus Peel by Induced Electrospray Ionization

IF 2.5 3区教育学 Q2 CHEMISTRY, MULTIDISCIPLINARY Journal of Chemical Education Pub Date : 2024-05-30 DOI:10.1021/acs.jchemed.4c00027

Bin Xu, Yongyi Li and Zhenwei Wei*,

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Abstract

A laboratory experiment was designed for senior undergraduate/graduate students to introduce direct current-induced electrospray ionization (DC-iESI), a technique which allows in situ mass spectrometric (MS) analysis of components in microregions of citrus peels. The citrus peels exhibit distinct microregions, such as oil glands and the flavedo, each containing unique components. Students were guided to compare traditional ESI (flow injection) and DC-iESI for the analysis of components in oil glands and the flavedo. As the flow injection ESI experiment has a requirement for a minimum amount of sample, students had to extract components from bulk citrus peels for MS analysis, which failed to show any differences in the components in these two typical regions. In the DC-iESI experiment, students used nanoelectrospray emitters to freehand collect liquids directly from oil glands and the flavedo, allowing MS analysis of components in these two different microregions. By comparison of these two methods, students have the opportunity to realize the limitations of traditional ESI analysis and learn about using DC-iESI for in situ MS analysis. We believe that this training course can help students enhance their understanding of in situ analysis and broaden their appreciation of MS in modern applications.

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让学生熟悉直接质谱分析方法：通过诱导电喷雾离子化定位柑橘皮中的成分

我们为高年级本科生/研究生设计了一个实验室实验，介绍直流电诱导电喷雾离子化（DC-iESI）技术，该技术可对柑橘皮微区中的成分进行原位质谱分析。柑橘类果皮具有不同的微区，如油脂腺和黄酮，每个微区都含有独特的成分。学生们在指导下比较了传统 ESI（流动注射）和 DC-iESI 在分析油腺和黄酮中的成分方面的优势。由于流动注射式 ESI 实验对样品量有最低要求，学生们不得不从大量柑橘皮中提取成分进行 MS 分析，结果显示这两个典型区域的成分没有任何差异。在 DC-iESI 实验中，学生们使用纳米电喷雾发射器直接从油脂腺和黄皮中自由收集液体，从而可以对这两个不同微区中的成分进行 MS 分析。通过这两种方法的比较，学生们有机会认识到传统 ESI 分析的局限性，并学习使用 DC-iESI 进行原位 MS 分析。我们相信，本培训课程能帮助学生加深对原位分析的理解，拓宽他们对 MS 在现代应用中的认识。

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来源期刊

Journal of Chemical Education 化学-化学综合

CiteScore

5.60

自引率

50.00%

发文量

465

审稿时长

6.5 months

期刊介绍： The Journal of Chemical Education is the official journal of the Division of Chemical Education of the American Chemical Society, co-published with the American Chemical Society Publications Division. Launched in 1924, the Journal of Chemical Education is the world’s premier chemical education journal. The Journal publishes peer-reviewed articles and related information as a resource to those in the field of chemical education and to those institutions that serve them. JCE typically addresses chemical content, activities, laboratory experiments, instructional methods, and pedagogies. The Journal serves as a means of communication among people across the world who are interested in the teaching and learning of chemistry. This includes instructors of chemistry from middle school through graduate school, professional staff who support these teaching activities, as well as some scientists in commerce, industry, and government.