Emergence of Salmonella Infantis carrying the pESI-like plasmid from eggs in egg grading and packing plants in Korea

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food microbiology Pub Date : 2024-05-25 DOI:10.1016/j.fm.2024.104568
Min Beom Kim, Young Ju Lee
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Abstract

The plasmid of emerging S. Infantis (pESI) or pESI-like plasmid in Salmonella enterica Infantis are consistently reported in poultry and humans worldwide. However, there has been limited research on these plasmids of S. Infantis isolated from eggs. Therefore, this study aimed to analyze the prevalence and characteristics of S. Infantis carrying the pESI-like plasmid from eggs in egg grading and packing plants. In this study, the pESI-like plasmid was only detected in 18 (78.3%) of 23 S. Infantis isolates, and it was absent in the other 9 Salmonella serovars. In particular, S. Infantis isolates carrying the pESI-like plasmid showed the significantly higher resistance to β-lactams, phenicols, cephams, aminoglycosides, quinolones, sulfonamides, and tetracyclines than Salmonella isolates without the pESI-like plasmid (p < 0.05). Moreover, all S. Infantis isolates carrying the pESI-like plasmid were identified as extended-spectrum β-lactamase (ESBL) producer, harboring the blaCTX-M-65 and blaTEM-1 genes, and carried non-β-lactamase resistance genes (ant(3′′)-Ia, aph(4)-Ia, aac(3)-IVa, aph(3′)-Ic, sul1, tetA, dfrA14, and floR) against five antimicrobial classes. However, all isolates without the pESI-like plasmid only carried the blaTEM-1 gene among the β-lactamase genes, and either had no non-β-lactamase resistance genes or harbored non-β-lactamase resistance genes against one or two antimicrobial classes. Furthermore, all S. Infantis isolates carrying the pESI-like plasmid carried class 1 and 2 integrons and the aadA1 gene cassette, but none of the other isolates without the pESI-like plasmid harbored integrons. In particular, D87Y substitution in the gyrA gene and IncP replicon type were observed in all the S. Infantis isolates carrying the pESI-like plasmid but not in the S. Infantis isolates without the pESI-like plasmid. The distribution of pulsotypes between pESI-positive and pESI-negative S. Infantis isolates was clearly distinguished, but all S. Infantis isolates were classified as sequence type 32, regardless of whether they carried the pESI-like plasmid. This study is the first to report the characteristics of S. Infantis carrying the pESI-like plasmid isolated from eggs and can provide valuable information for formulating strategies to control the spread of Salmonella in the egg industry worldwide.

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韩国鸡蛋分级和包装厂出现携带类 pESI 质粒的 Infantis 沙门氏菌
新出现的.全球不断有报告称,家禽和人类体内存在新出现的.Infantis质粒(pESI)或.Infantis中的类pESI质粒。然而,关于从鸡蛋中分离出的.Infantis质粒的研究却很有限。因此,本研究旨在分析鸡蛋分级和包装厂中携带 pESI 样质粒的.Infantis 的流行率和特征。在本研究中,23 个.Infantis 分离物中只有 18 个(78.3%)检测到 pESI 样质粒。其他 9 个血清型均未检出 pESI 类质粒。特别是,.特别是,携带 pESI 样质粒的.Infantis 分离物对β-内酰胺类、酚类、头孢菌素类、氨基糖苷类、喹诺酮类、磺胺类和四环素类药物的耐药性明显高于未携带 pESI 样质粒的分离物(< 0.05)。此外,所有.此外,所有携带 pESI 样质粒的.Infantis 分离物均被鉴定为扩谱β-内酰胺酶(ESBL)产生者,携带和基因,并携带针对五类抗菌药物的非β-内酰胺酶耐药基因(、、、、、和)。然而,所有没有 pESI 样质粒的分离物只携带 β-内酰胺酶基因中的基因,要么没有非 β-内酰胺酶耐药基因,要么携带针对一种或两种抗菌素的非 β-内酰胺酶耐药基因。此外,所有.此外,所有携带 pESI 样质粒的.Infantis 分离物都携带 1 类和 2 类整合子及基因盒,但没有携带 pESI 样质粒的其他分离物都不携带整合子。特别是,在所有携带类 pESI 质粒的.Infantis 分离物中都观察到了基因中的 D87Y 替换和 IncP 复制子类型,而在不携带类 pESI 质粒的.Infantis 分离物中却没有观察到。在 pESI 阳性和 pESI 阴性的.Infantis 分离物中,脉冲型的分布明显不同。但所有.无论是否携带 pESI 样质粒,所有.Infantis 分离物都被归类为序列类型 32。这项研究首次报告了从鸡蛋中分离出的携带类 pESI 质粒的.Infantis 的特征,可为制定控制.Infantis 在全球鸡蛋行业中传播的策略提供有价值的信息。
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来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
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