{"title":"Emergence of Salmonella Infantis carrying the pESI-like plasmid from eggs in egg grading and packing plants in Korea","authors":"Min Beom Kim, Young Ju Lee","doi":"10.1016/j.fm.2024.104568","DOIUrl":null,"url":null,"abstract":"<div><p>The plasmid of emerging <em>S</em>. Infantis (pESI) or pESI-like plasmid in <em>Salmonella enterica</em> Infantis are consistently reported in poultry and humans worldwide. However, there has been limited research on these plasmids of <em>S</em>. Infantis isolated from eggs. Therefore, this study aimed to analyze the prevalence and characteristics of <em>S</em>. Infantis carrying the pESI-like plasmid from eggs in egg grading and packing plants. In this study, the pESI-like plasmid was only detected in 18 (78.3%) of 23 <em>S</em>. Infantis isolates, and it was absent in the other 9 <em>Salmonella</em> serovars. In particular, <em>S</em>. Infantis isolates carrying the pESI-like plasmid showed the significantly higher resistance to β-lactams, phenicols, cephams, aminoglycosides, quinolones, sulfonamides, and tetracyclines than <em>Salmonella</em> isolates without the pESI-like plasmid (<em>p</em> < 0.05). Moreover, all <em>S</em>. Infantis isolates carrying the pESI-like plasmid were identified as extended-spectrum β-lactamase (ESBL) producer, harboring the <em>bla</em><sub>CTX-M-65</sub> and <em>bla</em><sub>TEM-1</sub> genes, and carried non-β-lactamase resistance genes (<em>ant(3′′)-Ia</em>, <em>aph(4)-Ia</em>, <em>aac(3)-IVa</em>, <em>aph(3′)-Ic</em>, <em>sul1</em>, <em>tetA</em>, <em>dfrA14</em>, and <em>floR</em>) against five antimicrobial classes. However, all isolates without the pESI-like plasmid only carried the <em>bla</em><sub>TEM-1</sub> gene among the β-lactamase genes, and either had no non-β-lactamase resistance genes or harbored non-β-lactamase resistance genes against one or two antimicrobial classes. Furthermore, all <em>S</em>. Infantis isolates carrying the pESI-like plasmid carried class 1 and 2 integrons and the <em>aadA1</em> gene cassette, but none of the other isolates without the pESI-like plasmid harbored integrons. In particular, D87Y substitution in the <em>gyrA</em> gene and IncP replicon type were observed in all the <em>S</em>. Infantis isolates carrying the pESI-like plasmid but not in the <em>S</em>. Infantis isolates without the pESI-like plasmid. The distribution of pulsotypes between pESI-positive and pESI-negative <em>S</em>. Infantis isolates was clearly distinguished, but all <em>S</em>. Infantis isolates were classified as sequence type 32, regardless of whether they carried the pESI-like plasmid. This study is the first to report the characteristics of <em>S</em>. Infantis carrying the pESI-like plasmid isolated from eggs and can provide valuable information for formulating strategies to control the spread of <em>Salmonella</em> in the egg industry worldwide.</p></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"122 ","pages":"Article 104568"},"PeriodicalIF":4.5000,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0740002024001060","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The plasmid of emerging S. Infantis (pESI) or pESI-like plasmid in Salmonella enterica Infantis are consistently reported in poultry and humans worldwide. However, there has been limited research on these plasmids of S. Infantis isolated from eggs. Therefore, this study aimed to analyze the prevalence and characteristics of S. Infantis carrying the pESI-like plasmid from eggs in egg grading and packing plants. In this study, the pESI-like plasmid was only detected in 18 (78.3%) of 23 S. Infantis isolates, and it was absent in the other 9 Salmonella serovars. In particular, S. Infantis isolates carrying the pESI-like plasmid showed the significantly higher resistance to β-lactams, phenicols, cephams, aminoglycosides, quinolones, sulfonamides, and tetracyclines than Salmonella isolates without the pESI-like plasmid (p < 0.05). Moreover, all S. Infantis isolates carrying the pESI-like plasmid were identified as extended-spectrum β-lactamase (ESBL) producer, harboring the blaCTX-M-65 and blaTEM-1 genes, and carried non-β-lactamase resistance genes (ant(3′′)-Ia, aph(4)-Ia, aac(3)-IVa, aph(3′)-Ic, sul1, tetA, dfrA14, and floR) against five antimicrobial classes. However, all isolates without the pESI-like plasmid only carried the blaTEM-1 gene among the β-lactamase genes, and either had no non-β-lactamase resistance genes or harbored non-β-lactamase resistance genes against one or two antimicrobial classes. Furthermore, all S. Infantis isolates carrying the pESI-like plasmid carried class 1 and 2 integrons and the aadA1 gene cassette, but none of the other isolates without the pESI-like plasmid harbored integrons. In particular, D87Y substitution in the gyrA gene and IncP replicon type were observed in all the S. Infantis isolates carrying the pESI-like plasmid but not in the S. Infantis isolates without the pESI-like plasmid. The distribution of pulsotypes between pESI-positive and pESI-negative S. Infantis isolates was clearly distinguished, but all S. Infantis isolates were classified as sequence type 32, regardless of whether they carried the pESI-like plasmid. This study is the first to report the characteristics of S. Infantis carrying the pESI-like plasmid isolated from eggs and can provide valuable information for formulating strategies to control the spread of Salmonella in the egg industry worldwide.
期刊介绍:
Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.