Food microbiology最新文献

{"title":"Optimizing Monascus pigment production: Insights from mycelial morphology, gene expression, and transcriptomic analysis in simulated seawater fermentation","authors":"Wenqian Zhao ,&nbsp;Tao Li ,&nbsp;Mei Pang,&nbsp;Xihong Zhao,&nbsp;Ting Hu,&nbsp;Xueyan Sun,&nbsp;Gong Chen","doi":"10.1016/j.fm.2025.104797","DOIUrl":"10.1016/j.fm.2025.104797","url":null,"abstract":"<div><div><em>Monascus</em> pigments (MPs) are secondary metabolites produced by <em>Monascus</em> spp., which can be significantly influenced by the extreme environment. In this work, the regulatory mechanism of MPs in simulated seawater fermentation (SSF) were investigated following mycelial morphology, gene expression, and transcriptomic analysis. Yield of the extracellular yellow pigments (EYPs) was significantly increased by 34.2 % in SSF, compared with the conventional fermentation (CF). The relative proportion of four EYPs (Y1/Y2-Y4) and the relative content of intracellular orange pigments to yellow pigments (O/Y) were also significantly (<em>p</em> &lt; 0.05) changed. Fluorescence inverted microscope (FIM) and field emission scanning electron microscope (FE-SEM) showed the mycelium morphology was regulated in better status to facilitate the metabolism and secretion of MPs in SSF. The pigment biosynthesis gene <em>MpFasA2</em>, <em>MpFasB2</em>, <em>MpPKS5, mppB</em>, <em>mppC</em>, <em>mppD,</em> and <em>mppE</em> were significantly (<em>p</em> &lt; 0.05) up-regulated, whereas the regulatory genes <em>mppR1</em>, <em>mppR2</em> were significantly (<em>p</em> &lt; 0.05) down-regulated in SSF. Transcriptome further revealed 83 differentially expressed genes (DEGs) between the two groups (CF vs SSF), with 40 up-regulated and 43 down-regulated. Among them, polyketide synthase genes and fatty acid oxidative degradation pathways related to pigment synthesis were significantly up-regulated in SSF, which promoted the metabolism of MPs. The down-regulation of DNA replication pathway indicated a slowdown in cell growth and differentiation, which keeping a favorable state for MPs synthesis. Biometabolism-related pathways of cell wall component and secretion-related pathways were also significantly regulated to accelerate the transmembrane transport of EYPs. This study may provide clues to clarify the response mechanism of high osmotic tolerance of <em>Monascus</em> spp.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"131 ","pages":"Article 104797"},"PeriodicalIF":4.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"D-tagatose biotransformation from lactose by lactase and recombinant Bacillus subtilis expressing l-arabinose isomerase, and the product separation, purification and crystallization","authors":"Xin Wen ,&nbsp;Huibin Lin ,&nbsp;Guangwen Liu ,&nbsp;Yuhang Ning ,&nbsp;Yilin Ren ,&nbsp;Can Li ,&nbsp;Chengjia Zhang ,&nbsp;Nannan Dong ,&nbsp;Jianqiang Lin ,&nbsp;Xin Song ,&nbsp;Guoqiang Zhuang ,&nbsp;Jianqun Lin","doi":"10.1016/j.fm.2025.104785","DOIUrl":"10.1016/j.fm.2025.104785","url":null,"abstract":"<div><div><span>d</span>-Tagatose is a low-calorie rare sugar with notable physiological benefits. The recombinant <em>Bacillus subtilis</em> expressing L-arabinose isomerase (LAI) was constructed and used together with lactase for biotransformation of <span>d</span>-tagatose from cheap substrate of lactose. Under optimum conditions, 45.6 g/L <span>d</span>-tagatose was produced from 200 g/L lactose, achieving a conversion yield of 0.228 g <span>d</span>-tagatose/g lactose. <span>d</span>-Glucose produced by lactose hydrolysis was removed by anaerobic fermentation with <em>Saccharomyces cerevisiae</em>. Subsequently, the sugar solution containing <span>d</span>-galactose and <span>d</span>-tagatose was decolorized by using powdered activated carbon, desalinated by anion and cation exchange resin beds, and separated by chromatographic column, resulting in decolorization rate of 95.5 %, desalinization rate of 93.8 % and <span>d</span>-tagatose solution purity of 97.9 %. Finally, the separated <span>d</span>-tagatose solution was concentrated and crystallized, resulting in <span>d</span>-tagatose crystals with 99.9 % purity. In summary, this paper establishes a complete bioprocess for <span>d</span>-tagatose from lactose catalyzed by using recombinant <em>B. subtilis</em> and lactase. The methods developed in this study show promise for mass production of food-grade <span>d</span>-tagatose from the inexpensive substrate lactose.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"131 ","pages":"Article 104785"},"PeriodicalIF":4.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Priestia megaterium PH3 in alleviating postharvest disease caused by Penicillium expansum in Nanguo pear fruit","authors":"Zilong Li ,&nbsp;Jiamin Jiang ,&nbsp;Keyu Sun ,&nbsp;Shuhong Ye","doi":"10.1016/j.fm.2025.104784","DOIUrl":"10.1016/j.fm.2025.104784","url":null,"abstract":"<div><div><em>Penicillium expansum</em> is one of the primary pathogens causing rot in fruit and vegetables. The progression of blue mold damages the appearance and nutritional value of produce, leading to significant economic losses. Recent studies have shown that endophytes, as a vital component of potential microbial agents, can control various fungal diseases. This study focuses on the inhibitory mechanism of the peanut endophyte <em>Priestia megaterium</em> PH3 against blue mold in Nanguo pear. The results demonstrated that the endophytic <em>P. megaterium</em> PH3 and its metabolites not only inhibit the spore germination of <em>P. expansum</em>. Most notably, after rapidly colonizing the surface of Nanguo pear fruit, <em>P. megaterium</em> PH3 enhances the activity of antioxidant enzymes (SOD, CAT, APX, MDHAR, DHAR and GR) and non-enzymatic antioxidants (AsA and GSH), suppressing the ROS surge caused by <em>P. expansum</em> infection. Additionally, the <em>P. megaterium</em> PH3 upregulates the activity of enzymes (PAL, C4H, 4CL, PPO and POD) and gene expression (<em>PuPAL</em>, <em>PuC4H</em> and <em>Pu4CL</em>) related to phenolics metabolism, promoting the synthesis and metabolism of phenolics and flavonoids compounds, effectively inhibiting the onset of blue mold in Nanguo pear. These findings indicate that <em>P. megaterium</em> PH3 is a promising and effective microbial agent for mitigating postharvest blue mold in Nanguo pear.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"131 ","pages":"Article 104784"},"PeriodicalIF":4.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical fields reverse FeSO4-induced VBNC state in Listeria monocytogenes and facilitate ferroptosis","authors":"Xiaolin Zhu,&nbsp;Yunhong Wang,&nbsp;Shurui Peng,&nbsp;Jiayi Zhang,&nbsp;Hongbo Li,&nbsp;Haizhen Mo,&nbsp;Liangbin Hu","doi":"10.1016/j.fm.2025.104796","DOIUrl":"10.1016/j.fm.2025.104796","url":null,"abstract":"<div><div>Non-thermal sterilization methods are effectively in eliminating foodborne pathogens while preserving the appearance and quality of food. In this study, three physical fields, magnetic field (MF), ultrasound (US), and blue light (BL), were introduced for their efficacy in sterilizing FeSO₄-induced viable but non-culturable (VBNC) <em>Listeria monocytogenes</em> (<em>L</em>. <em>monocytogenes</em>) cells over a 4 h treatment. The Fe-MF treatment induced <em>L</em>. <em>monocytogenes</em> cells to form VBNC state, resulting in an 80.13 % reduction in culturable cells, with a 90.58 % survival rate. The ultrasound-assisted FeSO₄ (Fe-US) treatment reduced the VBNC cell population by 52.63 %. In contrast, the blue light-assisted FeSO₄ (Fe-BL) treatment reversed the VBNC state toward culturable state at the node of 3 h, and induced irreversible 100 % cell death at 4 h, with a 94.73 % decrease in viability and 68.2 % membrane damage. Additionally, Fe-BL treatment led to cell wrinkling and secretion aggregation. BL treatment alone compromised membrane permeability and triggered intracellular protein aggregation. Mechanistic investigations revealed that BL-assisted treatment disrupted the protective mechanism of <em>L. monocytogenes</em> when induced by FeSO₄ to form VBNC state, compromised VBNC cell membranes, promoted intracellular Fe²⁺ accumulation, and induced a reactive oxygen species (ROS) burst and lipid peroxidation, ultimately leading to ferroptosis. Proteomic analysis identified 240 upregulated and 376 downregulated differentially expressed proteins, highlighting significant changes in pathways related to ribosome biosynthesis (related genes <em>rplJ</em> and <em>hpf</em> significant upregulated), intracellular iron homeostasis (<em>ctaB</em>, <em>hemN</em>, and <em>lmo2590</em> downregulated), cellular morphology (<em>mreB</em> and <em>tagH</em> downregulated), oxidative stress response (<em>lmo0720</em> and <em>lmo0799</em> downregulated), and DNA synthesis (<em>recA</em>, <em>dnaD</em>, <em>yidC2</em> and <em>fruB</em> downregulated). In conclusion, Fe-BL treatment effectively disrupted VBNC cell membranes, induced iron homeostasis imbalance, and triggered Fenton reaction-mediated ferroptosis. These findings provide a promising non-thermal sterilization strategy for inactivating VBNC <em>L. monocytogenes</em>, offering potential applications in food safety.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"131 ","pages":"Article 104796"},"PeriodicalIF":4.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of organic acid treatment on the microbial community composition of raw beef during extended refrigerated storage","authors":"Samuel C. Watson ,&nbsp;Nirosh D. Aluthge ,&nbsp;Rebecca A. Furbeck ,&nbsp;Samodha C. Fernando ,&nbsp;Byron D. Chaves ,&nbsp;Gary A. Sullivan","doi":"10.1016/j.fm.2025.104787","DOIUrl":"10.1016/j.fm.2025.104787","url":null,"abstract":"<div><div>Limiting bacterial spoilage and thereby protecting the economic value of raw beef destined for export is a top priority of the United States beef industry. Organic acid processing aids are commonly used to decrease pathogenic loads on raw beef, but knowledge of their efficacy against common spoilage bacteria is limited. Beef chuck rolls (IMPS 116A, N = 24) were obtained from two different processing facilities in Nebraska and treated with either 4.5 % lactic acid, 2.5 % Beefxide®, or 380 ppm peroxyacetic acid alongside a no-treatment control. Samples were stored at 2.7 °C for 112 days. Every 28 days, samples were evaluated using aerobic, anaerobic, psychrotrophic, lactic acid bacteria, and <em>Pseudomonas</em> plate counts and using amplicon sequencing of the V4 region of the 16S rRNA gene. Significant differences (P &lt; 0.05) between groups were determined using DeSeq2. <em>Lactococcus</em> became the most abundant genus on day 28 and every subsequent sampling point regardless of treatment group. <em>Pseudomonas</em> and <em>Yersinia</em> were also present at perceptible levels and were identified to be higher in control samples compared to lactic acid treated samples through differential abundance analysis. Concentrations of culturable bacteria increased during storage across all plating methods (P &lt; 0.05), but treatment differences were minimal. Overall, these treatments had impact on the bacterial diversity during storage. When considering the use of processing aids to limit spoilage, the treatment should be chosen based on a targeted specific spoilage organism.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"131 ","pages":"Article 104787"},"PeriodicalIF":4.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of radio frequency inactivation on bacterial DNA and membrane integrity in meat-based food model systems with various microstructures","authors":"Julian Espitia ,&nbsp;Davy Verheyen ,&nbsp;Shubhangi Mehrotra ,&nbsp;Dmytro S. Kozak ,&nbsp;Jan F.M. Van Impe","doi":"10.1016/j.fm.2025.104786","DOIUrl":"10.1016/j.fm.2025.104786","url":null,"abstract":"<div><div>Radio Frequency (RF) bacterial inactivation has been successfully used in several food products. However, RF dielectric heating can be affected by several factors, including food composition. Changes in the food matrix can not only influence microbial inactivation but also potentially alter RF inactivation mechanisms. Despite this, limited studies have been conducted to understand RF heating in different food matrices and its inactivation mechanisms. In the present study, three meat-based model systems were used (Liquid, Emulsion and Aqueous gel) for RF inactivation of <em>Listeria monocytogenes</em> and <em>S</em><em>almonella</em> Typhimurium at 27.12 MHz. The presence of fat and gel in the matrix increased <em>L. monocytogenes</em> heat resistance only at mild temperatures (&lt;60 °C) compared to liquid. On the contrary, <em>S.</em> Typhimurium inactivation rate was always slower in the presence of fat and gel in the matrix than in liquid during the whole RF treatment. dsDNA integrity did not show significant differences between treated and untreated samples, while cell membrane was damaged during RF heating for both microorganisms. Thus, RF microbial inactivation can be directly affected depending on the organisms and the medium complexity (e.g. fat, gel). Regarding RF inactivation mechanisms at 27.12 MHz, this study found no evidence of nonthermal effects. However, further research at the microscopic level is needed in order to better understand RF inactivation mechanisms.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"131 ","pages":"Article 104786"},"PeriodicalIF":4.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food products confiscated from air passengers travelling from third countries into the European Union: Microbiological analyses and genomic characterization of zoonotic and multiresistant bacteria","authors":"Nicola Rinn ,&nbsp;Anja Müller ,&nbsp;Ann-Sophie Braun ,&nbsp;Gabriel Greif ,&nbsp;Dagmar Stiefel ,&nbsp;Corinna Kehrenberg","doi":"10.1016/j.fm.2025.104783","DOIUrl":"10.1016/j.fm.2025.104783","url":null,"abstract":"<div><div>Illegal imports of food of animal origin from third countries into the EU are a potential transmission route for zoonotic and multiresistant bacterial pathogens. Here, we collected illegally imported food products that were confiscated from passengers arriving from non-EU countries at Frankfurt International Airport (FRA), Germany. A total of 100 food samples were microbiologically tested for the presence of foodborne and multiresistant pathogens and hygiene parameters were determined. For this, samples were qualitatively examined for <em>Salmonella</em> spp., <em>Listeria monocytogenes</em>, <em>Staphylococcus aureus</em>, Shiga toxin-producing <em>Escherichia coli</em> (STEC), methicillin-resistant <em>S. aureus</em> (MRSA), and extended-spectrum β-lactamase (ESBL)-/AmpC-producing Enterobacterales<em>.</em> Quantitative microbiological analyses included aerobic mesophilic colony counts, <em>L. monocytogenes</em>, coagulase-positive staphylococci, Enterobacterales and <em>E. coli</em> counts. Overall, Enterobacterales and <em>E. coli</em> were detected in 36 % and 23 % of the samples, respectively, indicating hygiene deficiencies, while foodborne pathogens were observed in 17 % of the samples. Selected isolates were subjected to a comprehensive genotypic analysis, for which they were whole genome sequenced. It was demonstrated that the <em>S. aureus</em> isolates (n = 11) revealed a wide variety of genotypic profiles, with one isolate belonging to a newly assigned sequence type ST8323. Three <em>S. aureus</em> isolates were classified as multiresistant, including one MRSA. The <em>Salmonella enterica</em> isolates (n = 3) belonged to three serovars (Uganda, Altona, Rauform) and were susceptible to all antimicrobial agents tested. Further, no resistance was detected in the obtained <em>L. monocytogenes</em> isolates (n = 3; ST8, ST121, ST425). In three of the 23 presumed commensal <em>E. coli</em> isolates multiresistance was observed, whereas the single STEC isolate (O43:H2) was susceptible. In addition, we obtained five ESBL-producing Enterobacterales isolates, of which one isolate carried a rarely described <em>bla</em>_SHV-168 subtype. Overall, the data show that illegal imports in the baggage of airline passengers can facilitate the spread of zoonotic and multiresistant bacterial isolates, including those resistant to third-generation cephalosporins and quinolones. This contributes to the transmission of newly described or uncommon lineages.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"131 ","pages":"Article 104783"},"PeriodicalIF":4.5,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting and preventing the next viral disease transmitted through food","authors":"Jeffrey T. LeJeune","doi":"10.1016/j.fm.2025.104782","DOIUrl":"10.1016/j.fm.2025.104782","url":null,"abstract":"<div><div>The ability of viruses to infect humans following oral exposure and disrupt normal physiological or anatomical functions is a hallmark of their potential to cause foodborne disease. While the etiology of the vast majority of foodborne diseases remains undetermined, viruses are often identified as the culprit when the cause is ascertained. Many undiagnosed causes of foodborne illnesses, especially sporadic cases, may go undetected or be caused by yet-to-be-identified viruses. The potential for food to become a transmission vehicle for viral diseases that are not typically acquired following ingestion may be described within the epidemiological paradigm. This model considers the characteristics and interactions of the host (the human), the agent (the virus), and the environment (the food, the food producing animal or the food production environment). Importantly, these factors are not static and evolution of viruses, transformations in agrifood systems, and changes in environmental conditions and human health and behaviour may contribute to increased pathogenicity, virulence, or exposure. In the context of determining the potential for additional viruses to emerge as important causes of foodborne disease, factors that contribute to hazard characterization (e.g., receptor affinity and distribution) and exposure assessment (e.g., prevalence in food animals and food hygiene) are reviewed. Although it is not possible to predict the type, the timing nor the location of the emergence of the next important cause of foodborne viral disease, the deployment and implementation of actions and behaviours related to personal and food hygiene, sanitation, and safe food handling practices can reduce the likelihood and impact of known and emergent viruses on the safety of the food supply and human health.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"130 ","pages":"Article 104782"},"PeriodicalIF":4.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VBNC induction and persistence of Listeria monocytogenes Scott A as a defence mechanism against free chlorine stress","authors":"Marianna Arvaniti ,&nbsp;Athanasios Balomenos ,&nbsp;Panagiotis Tsakanikas ,&nbsp;Panagiotis Skandamis","doi":"10.1016/j.fm.2025.104781","DOIUrl":"10.1016/j.fm.2025.104781","url":null,"abstract":"<div><div>Sodium hypochlorite (SH) belongs to the chlorine-releasing agents (CRAs) and is widely used as a disinfectant or a bleaching agent for sanitizing in the food processing environment and fresh-cut industry. In the present study, the potential induction of dormancy states, i.e. the VBNC state and persistence, in <em>Listeria monocytogenes</em>, Scott A strain, was evaluated after exposure to SH for 3 h at 20 °C. Our results showed that the concentration of free chlorine after cells (10^9.5 CFU/mL) resuspension into the working solution decreased down to 3.7 ppm (SD ± 0.4 ppm; pH 6.64 ± 0.1). To detect VBNC fractions we evaluated comparatively the results of plate counting with fluorescence microscopy, using 5(6)-carboxy-fluorescein diacetate (CFDA; metabolic activity) and propidium iodide (PI; death) staining. The resuscitation capacity of <em>L. monocytogenes</em> stressed single cells was monitored real-time on TSAYE at 37°C, using time-lapse microscopy. Thus, colony outgrowth kinetics were estimated and non-diving fractions were detected. Furthermore, variability in the division time per generation was examined. Our analyses showed that SH induces the VBNC state and persistence in <em>L. monocytogenes</em>. Phenotypic variants of “high” fitness, i.e. size colony variations (SCVs) were also detected in response to SH stress. <em>L. monocytogenes</em> cells presented a prolonged lag time after exposure to SH. This phenomenon is a defence mechanism that allows cells to tolerate stress and maximize population fitness. The investigation of the VBNC state is of high importance for the food industry, as the impacts of VBNC induction and single cell outgrowth heterogeneity can contribute to false-negative detection outcomes.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"130 ","pages":"Article 104781"},"PeriodicalIF":4.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the evolution of microbial communities from the phyllosphere and carposphere to the grape must of Vitis vinifera L. cv's Chardonnay and Pinot noir","authors":"Amber Justine Africa ,&nbsp;Mathabatha Evodia Setati ,&nbsp;Arina Corli Hitzeroth ,&nbsp;Erna Hailey Blancquaert","doi":"10.1016/j.fm.2025.104780","DOIUrl":"10.1016/j.fm.2025.104780","url":null,"abstract":"<div><div>Microbial communities associated with the grapevine phyllosphere and carposhere are a fundamental determinant of grape and wine quality. High throughput amplicon sequencing was used to profile the fungal and bacterial communities on the associated phylloplane and carposphere of <em>Vitis vinifera</em> L. cv's Chardonnay and Pinot noir in the Elgin and Hemel-en-Aarde wine districts of South Africa in the 2021–2022 growing season. The subsequent grape must was analysed to determine the prevalent microbiome. The most abundant bacterial and fungal genera found in both the phylloplane and carposphere of Chardonnay and Pinot noir were <em>Pseudomonas</em> and <em>Filobasidium</em>. The LEfSe (Linear discriminant analysis Effect Size) revealed significant differences in fungal and bacterial biomarkers from leaf, berry and grape must samples; however, no biomarkers were identified for cultivar nor location. Fungal β-diversity was significantly similar at different phenological stages, whereas bacterial β-diversity was significantly similar regardless of the site of colonisation. However, skin integrity of the grapes was may have influenced the microbial diversity.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"130 ","pages":"Article 104780"},"PeriodicalIF":4.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}