{"title":"Phloretin Ameliorates against Bisphenol A Induced Polycystic Ovarian Syndrome, an Endocrine Disrupting Chemical, in Prepubertal Rats","authors":"Nilufer Coşkun, Nurhayat Barlas","doi":"10.1134/s1062359022601173","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Polycystic ovary syndrome (PCOS) is an endocrine disorder whose etiology is not well understood and affecting 9–18% of women in reproductive age. We investigated the protective effects of phloretin against the PCOS, induced Bisphenol A (BPA) on prepubertal rat ovaries. This study started on female rats on the postnatal 28th day via subcutaneous injection by dissolving the compounds in corn oil at 30 min intervals, starting with phloretin, and followed by BPA. The dose of BPA was 50 mg/kg/day, and the doses of phloretin were 0.5, 5, and 50 mg/kg/day. Treatments were administered every day for 15 days. Likewise corn oil, BPA and testosterone propionate were given by the same way. BPA exposed groups, it has been observed that there are many cystic follicles in the ovary and their morphology has changed. Similarly, prolonged diestrus phases was observed in the smear cycle. The CYP11A1 and CYP17A enzymes stained intensively on the BPA group whereas these enzymes were stained weakly in the BPA + 5 and 50 mg/kg phloretin groups. In PCOS, induced by BPA, the cystic follicles were recorded on the ovaries whereas it was decreased in the BPA + phloretin groups. Prepubertal exposure to BPA was associated with decreased serum luteinizing hormone (LH), and follicle stimulating hormone (FSH) levels, increased estrogen (ERRα) and dihydrotestosterone (DHT) levels in testosterone propiyonat and BPA + phloretin (0.5 mg/kg/day) groups. In conclusion, in rat model, phloretin has positive effects against BPA induced polycystic ovarian syndrome, also it caused positive effects on ovarian tissue and hormone secretion.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology Bulletin","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1062359022601173","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOLOGY","Score":null,"Total":0}
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Abstract
Polycystic ovary syndrome (PCOS) is an endocrine disorder whose etiology is not well understood and affecting 9–18% of women in reproductive age. We investigated the protective effects of phloretin against the PCOS, induced Bisphenol A (BPA) on prepubertal rat ovaries. This study started on female rats on the postnatal 28th day via subcutaneous injection by dissolving the compounds in corn oil at 30 min intervals, starting with phloretin, and followed by BPA. The dose of BPA was 50 mg/kg/day, and the doses of phloretin were 0.5, 5, and 50 mg/kg/day. Treatments were administered every day for 15 days. Likewise corn oil, BPA and testosterone propionate were given by the same way. BPA exposed groups, it has been observed that there are many cystic follicles in the ovary and their morphology has changed. Similarly, prolonged diestrus phases was observed in the smear cycle. The CYP11A1 and CYP17A enzymes stained intensively on the BPA group whereas these enzymes were stained weakly in the BPA + 5 and 50 mg/kg phloretin groups. In PCOS, induced by BPA, the cystic follicles were recorded on the ovaries whereas it was decreased in the BPA + phloretin groups. Prepubertal exposure to BPA was associated with decreased serum luteinizing hormone (LH), and follicle stimulating hormone (FSH) levels, increased estrogen (ERRα) and dihydrotestosterone (DHT) levels in testosterone propiyonat and BPA + phloretin (0.5 mg/kg/day) groups. In conclusion, in rat model, phloretin has positive effects against BPA induced polycystic ovarian syndrome, also it caused positive effects on ovarian tissue and hormone secretion.
摘要多囊卵巢综合征（PCOS）是一种内分泌失调症，其病因尚不十分清楚，影响9-18%的育龄妇女。我们研究了多囊卵巢综合征诱导的双酚 A（BPA）对青春期前大鼠卵巢的保护作用。这项研究从雌性大鼠出生后第 28 天开始，通过皮下注射的方式，将化合物溶解在玉米油中，每隔 30 分钟注射一次。双酚 A 的剂量为 50 毫克/千克/天，紫檀素的剂量分别为 0.5、5 和 50 毫克/千克/天。每天给药，连续 15 天。同样，玉米油、双酚 A 和丙酸睾酮也以同样的方式给药。在暴露于双酚 A 的组别中，观察到卵巢中有许多囊性卵泡，其形态也发生了变化。同样，在涂片周期中也观察到发情期延长。在双酚 A 组中，CYP11A1 和 CYP17A 酶的染色较强，而在双酚 A + 5 和 50 毫克/千克磷脂组中，这些酶的染色较弱。在双酚 A 诱导的多囊卵巢综合征中，卵巢上出现了囊性卵泡，而在双酚 A + 植物提取物组中囊性卵泡减少。青春期前暴露于双酚 A 与血清黄体生成素（LH）和促卵泡激素（FSH）水平下降有关，睾酮丙酮纳组和双酚 A + 植物提取物（0.5 毫克/千克/天）组的雌激素（ERRα）和双氢睾酮（DHT）水平升高。总之，在大鼠模型中，保列汀对双酚 A 诱导的多囊卵巢综合征有积极作用，对卵巢组织和激素分泌也有积极影响。
期刊介绍：
Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.
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