Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newborn.

IF 2 4区 医学 Q3 HEMATOLOGY Hematology Pub Date : 2024-12-01 Epub Date: 2024-06-03 DOI:10.1080/16078454.2024.2360339
Shipeng Zhang, Sijin Li, Xuan Meng, Jia Chen, Yan Tang, Xiaobin Li
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Abstract

Background: Hemolytic disease of the newborn (HDN) is a common condition that can have a severe impact on the health of newborns due to the hemolytic reactions it triggers. Although numerous studies have focused on understanding the pathogenesis of HDN, there are still many unanswered questions.

Methods: In this retrospective study, serum samples were collected from 15 healthy newborns and 8 infants diagnosed with hemolytic disease. The relationship between different metabolites and various IgG subtypes in Healthy, HDN and BLI groups was studied by biochemical technique and enzyme-linked immunosorbent assay (ELISA). Metabolomics analysis was conducted to identify the differential metabolites associated with HDN. Subsequently, Pearson's correlation analysis was used to determine the relation of these differential metabolites with IgG isoforms. The relationship between the metabolites and IgG subtypes was observed after treatment.

Results: The study results revealed that infants with hemolytic disease exhibited abnormal elevations in TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4 levels when compared to healthy newborns. Additionally, differences in metabolite contents were also observed. N, N-DIMETHYLARGININE showed negative correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4, while 2-HYDROXYBUTYRATE, AMINOISOBUTANOATE, Inosine, and ALLYL ISOTHIOCYANATE exhibited positive correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4. Through metabolomics-based research, we have discovered associations between differential metabolites and different IgG isoforms during the onset of HDN.

Conclusion: These findings suggest that changes in metabolite and IgG isoform levels are linked to HDN. Understanding the involvement of IgG isoforms and metabolites can provide valuable guidance for the diagnosis and treatment of HDN.

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基于代谢组学的新生儿溶血病中不同代谢物结合IgG同工酶的意义研究
背景:新生儿溶血病(HDN新生儿溶血病(HDN)是一种常见疾病,由于其引发的溶血反应会严重影响新生儿的健康。尽管许多研究都致力于了解 HDN 的发病机理,但仍有许多问题尚待解答:在这项回顾性研究中,采集了 15 名健康新生儿和 8 名确诊为溶血性疾病的婴儿的血清样本。通过生化技术和酶联免疫吸附试验(ELISA)研究了健康组、HDN 组和 BLI 组不同代谢物与各种 IgG 亚型之间的关系。代谢组学分析旨在确定与 HDN 相关的不同代谢物。随后,利用皮尔逊相关分析确定了这些差异代谢物与 IgG 同工酶的关系。治疗后观察代谢物与 IgG 亚型之间的关系:研究结果表明,与健康新生儿相比,溶血病婴儿的 TBA、IgG1、IgG2a、IgG2b、IgG3 和 IgG4 水平异常升高。此外,还观察到代谢物含量的差异。N,N-二甲基乙炔与 TBA、IgG1、IgG2a、IgG2b、IgG3 和 IgG4 呈负相关,而 2-羟基丁酸、氨异丁酸、肌苷和 ALLYL 异硫氰酸与 TBA、IgG1、IgG2a、IgG2b、IgG3 和 IgG4 呈正相关。通过基于代谢组学的研究,我们发现了在 HDN 发病期间不同代谢物与不同 IgG 同工酶之间的关联:这些发现表明,代谢物和 IgG 同工酶水平的变化与 HDN 有关。了解 IgG 同工酶和代谢物的参与可为 HDN 的诊断和治疗提供有价值的指导。
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来源期刊
Hematology
Hematology 医学-血液学
CiteScore
2.60
自引率
5.30%
发文量
140
审稿时长
3 months
期刊介绍: Hematology is an international journal publishing original and review articles in the field of general hematology, including oncology, pathology, biology, clinical research and epidemiology. Of the fixed sections, annotations are accepted on any general or scientific field: technical annotations covering current laboratory practice in general hematology, blood transfusion and clinical trials, and current clinical practice reviews the consensus driven areas of care and management.
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