Immunoexpression of IL-33 in the different clinical aspects of canine atopic dermatitis

IF 1.4 3区 农林科学 Q4 IMMUNOLOGY Veterinary immunology and immunopathology Pub Date : 2024-05-26 DOI:10.1016/j.vetimm.2024.110786
Fernanda Borek , Seigo Nagashima , Wendie Roldán Villalobos , Vanessa Cunningham Gmyterco , Tássia Sell , Marconi Rodrigues de Farias , Gervásio Henrique Bechara
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Abstract

Canine atopic dermatitis (CAD) is a chronic and inflammatory skin condition with a multifaceted origin, involving genetic factors, skin barrier abnormalities, immune responses, and hypersensitivity to various allergens. Interleukin 33 (IL-33), released by keratinocytes upon cellular injury, plays a crucial role in atopic dermatitis pathogenesis by inducing Th2 lymphocyte-mediated immune responses. This study aimed to evaluate IL-33 expression in dogs with atopic dermatitis and compare it to a control group. Forty-nine dogs were included, with 39 having atopic dermatitis, subdivided into groups based on clinical characteristics, and ten in the control group. Lesion and pruritus scores were assessed, and incisional biopsies were analyzed for dermatopathological characteristics. IL-33 expression was evaluated using immunohistochemistry, the analyses were blinded, based on the measurement of immunostaining areas using Image Pro-Plus software, version 4.5, relying on a semi-automatic color segmentation method, where the tissue immunostaining area for each biomarker was artificially delimited and quantified. Statistically significant differences in IL-33 immunostaining were found among groups (P=0.0005). Lichenified dogs (group 4) exhibited higher immunostaining compared to erythema (group 3) (P=0.0006), alesional pruritus (group 2) (P=0.0261), and the control group (group 1) (P=0.0079). IL-33 immunostaining increased with lesion progression, strongly correlating with lesion scores (P<0.0001), particularly in patients with chronic lesions characterized by erythema and lichenification. These findings suggest IL-33's significant role in canine atopic dermatitis pathogenesis and its association with lesion and inflammation scores during the chronic phase. This suggests potential therapeutic interventions targeting IL-33 or its receptors, though further studies are needed to explore these possibilities.

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IL-33 在犬特应性皮炎不同临床表现中的免疫表达。
犬特应性皮炎(CAD)是一种慢性炎症性皮肤病,其发病原因是多方面的,涉及遗传因素、皮肤屏障异常、免疫反应以及对各种过敏原的超敏反应。白细胞介素 33(IL-33)在细胞损伤时由角质形成细胞释放,通过诱导 Th2 淋巴细胞介导的免疫反应在特应性皮炎的发病机制中起着至关重要的作用。本研究旨在评估特应性皮炎犬体内 IL-33 的表达,并将其与对照组进行比较。研究共纳入了 49 只狗,其中 39 只患有特应性皮炎,根据临床特征分为不同的组别,10 只为对照组。对皮损和瘙痒评分进行了评估,并对切口活检组织进行了皮肤病理学特征分析。采用免疫组织化学方法评估 IL-33 的表达,分析是盲法,使用 Image Pro-Plus 4.5 版软件测量免疫染色区域,采用半自动颜色分割法,人为划定并量化每种生物标记物的组织免疫染色区域。各组之间的 IL-33 免疫染色差异有统计学意义(P=0.0005)。与红斑(第 3 组)(P=0.0006)、脓疱性瘙痒(第 2 组)(P=0.0261)和对照组(第 1 组)(P=0.0079)相比,苔藓化犬(第 4 组)的免疫染色更高。IL-33 免疫染色随着皮损的发展而增加,与皮损评分密切相关(P=0.0079)。
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来源期刊
CiteScore
3.40
自引率
5.60%
发文量
79
审稿时长
70 days
期刊介绍: The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.
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