Dual release of daptomycin and BMP-2 from a composite of β-TCP ceramic and ADA gelatin.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Biotechnology Pub Date : 2024-06-03 DOI:10.1186/s12896-024-00863-4

Lucas Ritschl, Pia Schilling, Annette Wittmer, Annerose Serr, Hagen Schmal, Michael Seidenstuecker

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Abstract

Background: Antibiotic-containing carrier systems are one option that offers the advantage of releasing active ingredients over a longer period of time. In vitro sustained drug release from a carrier system consisting of microporous β-TCP ceramic and alginate has been reported in previous works. Alginate dialdehyde (ADA) gelatin gel showed both better mechanical properties when loaded into a β-TCP ceramic and higher biodegradability than pure alginate.

Methods: Dual release of daptomycin and BMP-2 was measured on days 1, 2, 3, 6, 9, 14, 21, and 28 by HPLC and ELISA. After release, the microbial efficacy of the daptomycin was verified and the biocompatibility of the composite was tested in cell culture.

Results: Daptomycin and the model compound FITC protein A (n = 30) were released from the composite over 28 days. A Daptomycin release above the minimum inhibitory concentration (MIC) by day 9 and a burst release of 71.7 ± 5.9% were observed in the loaded ceramics. Low concentrations of BMP-2 were released from the loaded ceramics over 28 days.

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从 β-TCP 陶瓷和 ADA 明胶的复合材料中双重释放达托霉素和 BMP-2。

背景：含抗生素的载体系统是一种可在较长时间内释放活性成分的选择。以前的研究曾报道过由微孔 β-TCP 陶瓷和海藻酸盐组成的载体系统的体外持续药物释放。与纯海藻酸盐相比，海藻酸盐二醛（ADA）凝胶在载入β-TCP陶瓷时显示出更好的机械性能和更高的生物降解性：在第 1、2、3、6、9、14、21 和 28 天，通过 HPLC 和 ELISA 测量达托霉素和 BMP-2 的双重释放。释放后，验证了达托霉素的微生物功效，并在细胞培养中测试了复合材料的生物相容性：结果：28 天内，达托霉素和模型化合物 FITC 蛋白 A（n = 30）从复合材料中释放出来。到第 9 天时，达托霉素的释放量超过了最低抑制浓度（MIC），负载陶瓷的猝灭释放量为 71.7 ± 5.9%。在 28 天内，负载陶瓷释放了低浓度的 BMP-2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.