Pub Date : 2026-03-24DOI: 10.1186/s12896-026-01140-2
Yingying Wang, Meihui Cao, Mei Hu, Hengrui Xu, Yafei Du, Changxing Sun, Lingfei Kong, Yu Luo, Xiaoyu Liu, Jianming Yang, Bo Liang
Background: 3-Hydroxypropionic acid (3-HP) is a promising C3 platform chemical with wide industrial applications. However, its microbial production remains limited by insufficient intracellular malonyl-CoA availability and metabolic imbalance.
Result: In this study, we systematically engineered Escherichia coli for enhanced 3-HP biosynthesis. The malonate assimilation genes (matB, smatPQM) and 3-HP biosynthesis gene (mcr) were chromosomally integrated using CRISPR/Cas9, resulting in a plasmid-free, antibiotic-free strain (WYY04) that produced 21.97 mM 3-HP, 0.51-fold higher than the plasmid-based system. Further improvement was achieved by CRISPRi-mediated repression of fatty acid biosynthesis genes (fabD, fabF), increasing 3-HP titer by 66%. Introduction of a malonyl-CoA-responsive FapR/fapO biosensor enabled dynamic regulation of mcr expression, enhancing 3-HP production by 59%. Through all these above engineering, the 3-HP production of the strain WYY19 increased by 2.29 times compared to that of the plasmid-expressing system. Under optimized fermentation conditions, the final engineered strain WYY19 produced 42.22 g/L 3-HP with the specific productivity of 0.69 g/g and 0.46 g/L/h from glucose and malonate in fed-batch bioreactor.
Conclusions: This study demonstrates a robust, genetically stable, and scalable microbial platform for 3-HP biosynthesis.
{"title":"Systematic engineering of Escherichia coli for biosynthesis of 3-hydroxypropionic acid from glucose and malonate.","authors":"Yingying Wang, Meihui Cao, Mei Hu, Hengrui Xu, Yafei Du, Changxing Sun, Lingfei Kong, Yu Luo, Xiaoyu Liu, Jianming Yang, Bo Liang","doi":"10.1186/s12896-026-01140-2","DOIUrl":"https://doi.org/10.1186/s12896-026-01140-2","url":null,"abstract":"<p><strong>Background: </strong>3-Hydroxypropionic acid (3-HP) is a promising C3 platform chemical with wide industrial applications. However, its microbial production remains limited by insufficient intracellular malonyl-CoA availability and metabolic imbalance.</p><p><strong>Result: </strong>In this study, we systematically engineered Escherichia coli for enhanced 3-HP biosynthesis. The malonate assimilation genes (matB, smatPQM) and 3-HP biosynthesis gene (mcr) were chromosomally integrated using CRISPR/Cas9, resulting in a plasmid-free, antibiotic-free strain (WYY04) that produced 21.97 mM 3-HP, 0.51-fold higher than the plasmid-based system. Further improvement was achieved by CRISPRi-mediated repression of fatty acid biosynthesis genes (fabD, fabF), increasing 3-HP titer by 66%. Introduction of a malonyl-CoA-responsive FapR/fapO biosensor enabled dynamic regulation of mcr expression, enhancing 3-HP production by 59%. Through all these above engineering, the 3-HP production of the strain WYY19 increased by 2.29 times compared to that of the plasmid-expressing system. Under optimized fermentation conditions, the final engineered strain WYY19 produced 42.22 g/L 3-HP with the specific productivity of 0.69 g/g and 0.46 g/L/h from glucose and malonate in fed-batch bioreactor.</p><p><strong>Conclusions: </strong>This study demonstrates a robust, genetically stable, and scalable microbial platform for 3-HP biosynthesis.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-21DOI: 10.1186/s12896-026-01124-2
Mohamed K Y Soliman, Mai M El-Ashmony, Hager A Bendary, Fatma Rasslan, Salem S Salem
{"title":"Phyto mediated synthesis of copper oxide nanoparticles with Zingiber officinale: comprehensive bioactivity assessment against bacterial pathogens, cancer, and viruses.","authors":"Mohamed K Y Soliman, Mai M El-Ashmony, Hager A Bendary, Fatma Rasslan, Salem S Salem","doi":"10.1186/s12896-026-01124-2","DOIUrl":"https://doi.org/10.1186/s12896-026-01124-2","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-17DOI: 10.1186/s12896-026-01131-3
Yunhee Chang, Jun-Young Park, Dasom Hwang, Min Tae Kim, Seyeon Yoon, Seung-Hak Cho
{"title":"A novel method to encapsulate polydeoxyribonucleotides extracted from trout into exosomes and applications in skin recovery.","authors":"Yunhee Chang, Jun-Young Park, Dasom Hwang, Min Tae Kim, Seyeon Yoon, Seung-Hak Cho","doi":"10.1186/s12896-026-01131-3","DOIUrl":"https://doi.org/10.1186/s12896-026-01131-3","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-17DOI: 10.1186/s12896-026-01134-0
Li Zhang, Juan Luo, Chuanguang Zhang, Hong Wang, Mingjie Yan, Naiming Zhang, Yunsheng Xia, Xianrong Yue
{"title":"Sustainable utilization of phosphogypsum in agriculture enhanced by mycorrhizal organisms: a new perspective.","authors":"Li Zhang, Juan Luo, Chuanguang Zhang, Hong Wang, Mingjie Yan, Naiming Zhang, Yunsheng Xia, Xianrong Yue","doi":"10.1186/s12896-026-01134-0","DOIUrl":"https://doi.org/10.1186/s12896-026-01134-0","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1186/s12896-026-01123-3
Wenting Xing, Dongmei Huang, Bin Wu, Funing Ma, Haijie Huang, Shun Song, Yi Xu
{"title":"Breaking the barrier of interspecific recalcitrance: a unified and efficient in vitro conservation system for Passiflora germplasm.","authors":"Wenting Xing, Dongmei Huang, Bin Wu, Funing Ma, Haijie Huang, Shun Song, Yi Xu","doi":"10.1186/s12896-026-01123-3","DOIUrl":"https://doi.org/10.1186/s12896-026-01123-3","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-13DOI: 10.1186/s12896-026-01119-z
Jannatul Firdous Siddique, G R Shree Kumari, Sudarshana Deepa Vijay Kumar, C Subathra Devi, Mohanasrinivasan Vaithilingam
{"title":"p53 mediated cell cytotoxicity and DNA damage in lung cancer cell line A549 triggered by a small peptide extracted from a probiotic strain Lacticaseibacillus casei.","authors":"Jannatul Firdous Siddique, G R Shree Kumari, Sudarshana Deepa Vijay Kumar, C Subathra Devi, Mohanasrinivasan Vaithilingam","doi":"10.1186/s12896-026-01119-z","DOIUrl":"https://doi.org/10.1186/s12896-026-01119-z","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号