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Berberine-ZnO loaded chitosan nanoparticles inhibits biofilm formation in Pseudomonas aeruginosa PAO1 through targeting extracellular polymeric substances. 载小檗碱氧化锌壳聚糖纳米颗粒通过靶向胞外聚合物物质抑制铜绿假单胞菌PAO1生物膜的形成。
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-02-03 DOI: 10.1186/s12896-026-01103-7
Fatemeh Esnaashari, Fatemeh Azizi Alidoust, Nastaran Jafari, Mohebat Ghasemi, Fatemeh Asadi Rahmani, Sara Sedaghat Safsari, Hossein Zahmatkesh
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引用次数: 0
Development of a nanoemulsion incorporating 3-acetyl-11-keto-β-boswellic acid (AKBA): solubility and oral bioavailability through in vitro and in vivo studies. 含3-乙酰-11-酮-β-乳香酸（AKBA）纳米乳的研制：通过体外和体内研究的溶解度和口服生物利用度
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-02-03 DOI: 10.1186/s12896-026-01099-0
Najmeh Ketabchi, Seyed Nasser Ostad, Mahmoud Ghazi-Khansari, Hossein Ghanbari, Fariba Esmaeili, Amir Amani

Background: 3-Acetyl-11-keto-β-boswellic acid (AKBA) has attracted considerable interest due to its therapeutic potential against inflammatory and cancer-related disorders. However, its poor oral bioavailability remains a critical limitation for clinical application. This study aimed to enhance the bioavailability of AKBA by developing a nanoemulsion (NE)-based delivery system.

Results: Several NE formulations were optimized and characterized based on drug loading, stability, and droplet size. The optimized NE-AKBA showed a droplet size of 12-15 nm, a low polydispersity index, and a zeta potential of - 14.5 mV. Permeability studies revealed significantly improved transport of NE-AKBA across Caco-2 monolayers within the first hour. Pharmacokinetic analysis revealed a notable increase in systemic exposure: Cmax increased from 3.36 to 12.23 µg/mL and AUC0 - t increased from 40.9 to 72.1 µg·h/mL, while Tmax remained unchanged at 6 h, indicating an enhanced extent of absorption without alteration of absorption kinetics.

Conclusion: These findings demonstrate that nanoemulsion-based delivery significantly improves the oral bioavailability of AKBA and provides a promising platform for its future therapeutic development.

背景：3-乙酰基-11-酮-β-乳香酸（AKBA）由于其治疗炎症和癌症相关疾病的潜力而引起了相当大的兴趣。然而，其较差的口服生物利用度仍然是临床应用的关键限制。本研究旨在通过开发纳米乳给药系统来提高AKBA的生物利用度。结果：根据载药量、稳定性和滴度对几种NE制剂进行了优化和表征。优化后的NE-AKBA液滴尺寸为12-15 nm，多分散性指数低，zeta电位为- 14.5 mV。渗透性研究表明，在第一个小时内，NE-AKBA通过Caco-2单层的运输显著改善。药代动力学分析显示，全身暴露量显著增加：Cmax从3.36增加到12.23µg/mL， AUC0 - t从40.9增加到72.1µg·h/mL，而Tmax在6 h时保持不变，表明吸收程度增强，但吸收动力学没有改变。结论：纳米乳化给药可显著提高AKBA的口服生物利用度，为其未来的治疗开发提供了良好的平台。
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引用次数: 0
Digital PCR-based characterization of certified genomic DNA reference materials from genetically modified papaya Huanong No. 1. 转基因木瓜华农1号基因组DNA标准物质的数字pcr鉴定
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-02-02 DOI: 10.1186/s12896-026-01105-5
Guiqin Yang, Zhiwen Pan, Shiran Zheng, Gongwen He, Lili Zhu, Caiyao Wu, Juan Yao, Liang Li, Dagang Jiang
{"title":"Digital PCR-based characterization of certified genomic DNA reference materials from genetically modified papaya Huanong No. 1.","authors":"Guiqin Yang, Zhiwen Pan, Shiran Zheng, Gongwen He, Lili Zhu, Caiyao Wu, Juan Yao, Liang Li, Dagang Jiang","doi":"10.1186/s12896-026-01105-5","DOIUrl":"https://doi.org/10.1186/s12896-026-01105-5","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Scaling the manufacture of adipose tissue-derived mesenchymal stromal cells: integrated bioreactor workflows from inoculation to harvest without the need of seed train. 脂肪组织来源间充质间质细胞的规模化生产：从接种到收获的一体化生物反应器工作流程，无需种子培养。
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-01-22 DOI: 10.1186/s12896-026-01101-9
Marta H G Costa, Ana Paula Terrasso, Inês E Crespo, Raúl Valero, Beatriz Menéndez, Beatriz Painho, Carolina D Sousa, Beatriz Gamelas, Hélio Tomás, Pablo Mancheno-Corvo, Ramón Menta, Carmen R Gugel, Vanessa Fernández-Gómez, Ángel Herrero-Méndez, Irene Zamora Mármol, Alvaro Avivar-Valderas, Laura M Pérez, Olga de la Rosa, Eleuterio Lombardo, Margarida Serra, Maitane Ortiz-Virumbrales
{"title":"Scaling the manufacture of adipose tissue-derived mesenchymal stromal cells: integrated bioreactor workflows from inoculation to harvest without the need of seed train.","authors":"Marta H G Costa, Ana Paula Terrasso, Inês E Crespo, Raúl Valero, Beatriz Menéndez, Beatriz Painho, Carolina D Sousa, Beatriz Gamelas, Hélio Tomás, Pablo Mancheno-Corvo, Ramón Menta, Carmen R Gugel, Vanessa Fernández-Gómez, Ángel Herrero-Méndez, Irene Zamora Mármol, Alvaro Avivar-Valderas, Laura M Pérez, Olga de la Rosa, Eleuterio Lombardo, Margarida Serra, Maitane Ortiz-Virumbrales","doi":"10.1186/s12896-026-01101-9","DOIUrl":"https://doi.org/10.1186/s12896-026-01101-9","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146028374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction and application of an insertional activation cloning vector. 插入激活克隆载体的构建与应用。
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-01-19 DOI: 10.1186/s12896-026-01102-8
Yan Zhang, Yun He, Yi Ding, Shanhua Lyu, Yinglun Fan
{"title":"Construction and application of an insertional activation cloning vector.","authors":"Yan Zhang, Yun He, Yi Ding, Shanhua Lyu, Yinglun Fan","doi":"10.1186/s12896-026-01102-8","DOIUrl":"https://doi.org/10.1186/s12896-026-01102-8","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microalgal extracts optimize lettuce quality through extraction-method-dependent modulation of primary and secondary metabolism. 微藻提取物通过对生菜初级和次级代谢的调节来优化生菜品质。
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-01-16 DOI: 10.1186/s12896-025-01097-8
Mutale Joan Chanda, Aimane Soidihane, Sana Mounaimi, Mohamed Hachimi Alaoui, Hassan Lamaden, Karim Lyamlouli, Hicham El Arroussi
{"title":"Microalgal extracts optimize lettuce quality through extraction-method-dependent modulation of primary and secondary metabolism.","authors":"Mutale Joan Chanda, Aimane Soidihane, Sana Mounaimi, Mohamed Hachimi Alaoui, Hassan Lamaden, Karim Lyamlouli, Hicham El Arroussi","doi":"10.1186/s12896-025-01097-8","DOIUrl":"https://doi.org/10.1186/s12896-025-01097-8","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145987843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of broad-host-range Salmonella phage GSP006 and its efficacy in controlling Salmonella Pullorum contamination in poultry feed and drinking water. 广宿主沙门氏菌噬菌体GSP006的鉴定及其对家禽饲料和饮用水中白痢沙门氏菌污染的控制效果
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-01-15 DOI: 10.1186/s12896-026-01100-w
Shenyu Pang, Hongyang Zhang, Xincong Liu, Jilai Wang, Shunyuan Pan, Xiangyu Kong, Jun Song, Dongyang Gao
{"title":"Characterization of broad-host-range Salmonella phage GSP006 and its efficacy in controlling Salmonella Pullorum contamination in poultry feed and drinking water.","authors":"Shenyu Pang, Hongyang Zhang, Xincong Liu, Jilai Wang, Shunyuan Pan, Xiangyu Kong, Jun Song, Dongyang Gao","doi":"10.1186/s12896-026-01100-w","DOIUrl":"https://doi.org/10.1186/s12896-026-01100-w","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145987905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Process parameters optimization of sweet orange juice ohmic heat-assisted vacuum evaporation and assessment of concentrate quality. 甜橙汁欧姆热真空蒸发工艺参数优化及浓缩液质量评价。
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-01-13 DOI: 10.1186/s12896-025-01094-x
Vasudeva Rao Vangapandu, Venkata S P Bitra
{"title":"Process parameters optimization of sweet orange juice ohmic heat-assisted vacuum evaporation and assessment of concentrate quality.","authors":"Vasudeva Rao Vangapandu, Venkata S P Bitra","doi":"10.1186/s12896-025-01094-x","DOIUrl":"10.1186/s12896-025-01094-x","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":"18"},"PeriodicalIF":3.4,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12879478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of gamma irradiation on lemongrass (Cymbopogon citratus): phytochemical content, antioxidant and antibacterial activity. γ辐射对柠檬草（Cymbopogon citratus）的影响：植物化学成分、抗氧化和抗菌活性。
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-01-09 DOI: 10.1186/s12896-025-01090-1
Wael El-Desouky Ibrahim, Nermien Z Ahmed
{"title":"Impact of gamma irradiation on lemongrass (Cymbopogon citratus): phytochemical content, antioxidant and antibacterial activity.","authors":"Wael El-Desouky Ibrahim, Nermien Z Ahmed","doi":"10.1186/s12896-025-01090-1","DOIUrl":"10.1186/s12896-025-01090-1","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":"13"},"PeriodicalIF":3.4,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12849302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145942408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Statistical optimization of fermentation conditions for enhanced production of Nattokinase from Bacillus subtilis MM26. 枯草芽孢杆菌MM26产纳豆激酶发酵条件的统计优化。
IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BMC Biotechnology
Pub Date : 2026-01-07 DOI: 10.1186/s12896-025-01093-y
M Maneesha, C Subathra Devi
{"title":"Statistical optimization of fermentation conditions for enhanced production of Nattokinase from Bacillus subtilis MM26.","authors":"M Maneesha, C Subathra Devi","doi":"10.1186/s12896-025-01093-y","DOIUrl":"10.1186/s12896-025-01093-y","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":"17"},"PeriodicalIF":3.4,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12870909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145917090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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