Unsaturated fatty acid, Nonacosenoic acid isolated from an endophyte Chaetomium nigricolor inhabiting the stem of Catharanthus roseus and its bioactivity

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-06-01 DOI:10.1016/j.funbio.2024.05.008

Geethanjali Dhayanithy , Somnath Mukherjee , Kamalraj Subban , Subathra Radhakrishnan , Jayabaskaran Chelliah

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Abstract

The endophytic fungus Chaetomium nigricolor culture filtrate's hexane extract was used to identify a cytotoxic very long-chain fatty acid. Based on multiple spectroscopic investigations, the structure of the compound was predicted to be an unsaturated fatty acid, Nonacosenoic acid (NA). Using the MTT assay, the compound's cytotoxic potential was evaluated against MCF-7, A-431, U-251, and HEK-293 T cells. The compound was moderately cytotoxic to breast carcinoma cell line, MCF-7 cells and negligibly cytotoxic to non-cancerous cell line HEK-293 T cells. The compound exhibited mild cytotoxic activity against A-431 and U-251 cells. The compound also induced ROS generation and mitochondrial depolarization in MCF-7 cells when assessed via the NBT and JC-1 assays, respectively. This is the first report on the production of nonacosenoic acid from the endophytic fungus Chaetomium nigricolor and the assessment of its bioactivity.

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从栖息于石竹科植物茎中的内生菌 Chaetomium nigricolor 中分离出的不饱和脂肪酸 Nonacosenoic 酸及其生物活性

研究人员利用内生真菌黑茶藨草（Chaetomium nigricolor）培养物滤液的正己烷提取物鉴定了一种具有细胞毒性的超长链脂肪酸。根据多种光谱研究，该化合物的结构被预测为一种不饱和脂肪酸--壬烯烯酸（NA）。利用 MTT 试验评估了该化合物对 MCF-7、A-431、U-251 和 HEK-293 T 细胞的细胞毒性潜力。该化合物对乳腺癌细胞株 MCF-7 细胞有中度细胞毒性，对非癌细胞 HEK-293 T 细胞的细胞毒性可忽略不计。该化合物对 A-431 和 U-251 细胞有轻微的细胞毒性。通过 NBT 和 JC-1 试验评估，该化合物还能分别诱导 MCF-7 细胞产生 ROS 和线粒体去极化。这是首次报道从内生真菌 Chaetomium nigricolor 中产生壬烯烯酸并对其生物活性进行评估。

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