Comparison of structural, mechanical, and histochemical characteristics of L-PRF and T-PRF membranes using different centrifugation protocols – An ex-vivo observational study

Abstract

Objectives

Many investigators are looking into the possibility of using platelet-rich. hemoderivatives as a source of the protein structures and cytokines necessary for. periodontal healing of wounds. The objective of the study was to evaluate and. compare the structural, mechanical, and histochemical characteristics of L-PRF and TPRF. membranes prepared by using three different centrifugation protocols.

Methods

Thirty participants around the ages of 20 and 35 who were in good overall. health had their antecubital veins pricked for ten milliliters of the blood. Venous blood. was centrifuged immediately after collection in silica-coated glass tubes and titaniumcoated. tubes utilizing the APRF machine. The mechanical properties of PRF. membranes (n = 30) for L-PRF and T-PRF each protocol) were assessed using a. desktop universal testing machine.

Results

The forces around 700 g (3200 rpm, 15 min) yielded a PRF membrane with. desirable mechano-degradation characteristics for its clinical application. Histochemical examination revealed mixed inflammatory infiltrate with predominantly. platelets followed by lymphocytes, neutrophils& monocytes in T-PRF. Whereas, in. LPRF thin strands of mixed inflammatory infiltrate predominantly neutrophils &. monocytes are seen in the connective tissue stroma.

Conclusion

This study demonstrated that centrifugation speed, force, and time. influenced the structural, and mechanical properties of PRF membranes prepared with. various protocols. This is the first study to reveal the marked impact of glass and. titanium-reinforced centrifugation tubes on the final production of PRF. It was further. found that a significant difference was observed between L-PRF and T-PRF at. 3200 rpm and 2700 rpm protocols.