Pathogenicity and colonization of Metrosideros polymorpha by Ceratocystis huliohia

Abstract

Both Ceratocystis lukuohia and C. huliohia have been associated with Rapid ʻŌhiʻa Death (ROD), an emerging threat to ʻōhiʻa (Metrosideros polymorpha), a keystone forest tree species. The vascular wilt disease caused by C. lukuohia has been recently described and is responsible for the widespread ROD epidemic on Hawai'i Island. However, the role of C. huliohia in ROD development and tree death is not clear. Artificial inoculation of field-grown ʻōhiʻa with C. huliohia and dissections of naturally infected, early symptomatic forest trees were conducted to confirm pathogenicity on field grown trees and the pattern of internal colonization. In two trials, crowns of trees with main stems inoculated with C. huliohia were visually healthy at the time of tree harvest after 43–55 days in the first trial, and after 91 days in the second trial. However, elliptical inner bark cankers underlain by reddish-brown xylem were associated with the inoculation points. Similar canker and stain symptoms were found on stems and branches of ʻōhiʻa (24–26 cm trunk diameter) naturally infected by C. huliohia. This xylem stain manifested as multiple distinct elliptical cankers or the coalescing of multiple cankers. The pathogen was commonly isolated from the perimeter of the stained outer sapwood and to a depth of 4 cm. The coalescence of multiple cankers was associated with the crown symptoms observed on the naturally infected forest trees that were dissected. Multiple C. huliohia infections that lead to coalescing cankers which subsequently girdle stems likely occurs over one or more years compared to the shorter time (e.g., months) required for C. lukuohia-caused death to occur.