Upconversion nanoparticles–based targeted imaging of MCF-7 breast cancer cells

IF 2.1 4区材料科学 Q3 CHEMISTRY, MULTIDISCIPLINARY Journal of Nanoparticle Research Pub Date : 2024-06-03 DOI:10.1007/s11051-024-06035-x

Meric Cansu Cinar, Mahla Shahsavar Gocmen, Aysegul Aciksari, Ramazan Ceylan, Seray Sahsuvar, Sibel Cetinel, Ozgul Gok, Ayse Dulda

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Abstract

Upconversion nanoparticles (UCNPs) doped with lanthanides are introduced as a significant tool in bioimaging applications. Here in, a comparative study has been performed to understand the cell internalization capacity of folic acid (FA) and arginine-glycine-aspartic acid-lysine (RGDK) ligands. To achieve this goal, polyacrylic acid (PAA) coated UCNPs (NaYF₄:Yb³⁺, Er³⁺) are conjugated with various surface ligands such as FA and RGDK through a straightforward ligand exchange procedure. Ligand conjugation to UCNPs was characterized with a transmission electron microscope (TEM), Fourier-transform infrared (FT-IR) spectroscopy, zeta potential measurements, nuclear magnetic resonance (NMR) spectroscopy, and NanoDrop measurements. The cellular uptake of the nanoparticles was investigated on the breast cancer MCF-7 cell line. The obtained results demonstrated that folic acid and RGDK functionalized UCNPs showed remarkably higher cellular uptake, which clearly indicates that the specific targeting of UCNPs provides a better quality of sub-cellular imaging at lower energy band region.

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基于上转换纳米粒子的 MCF-7 乳腺癌细胞靶向成像技术

掺杂镧系元素的上转换纳米粒子（UCNPs）是生物成像应用中的重要工具。在这里，我们进行了一项比较研究，以了解叶酸（FA）和精氨酸-甘氨酸-天冬氨酸-赖氨酸（RGDK）配体的细胞内化能力。为实现这一目标，通过直接的配体交换程序，将聚丙烯酸（PAA）包覆的 UCNPs（NaYF4:Yb3+, Er3+）与各种表面配体（如 FA 和 RGDK）共轭。通过透射电子显微镜（TEM）、傅立叶变换红外光谱（FT-IR）、ZETA 电位测量、核磁共振（NMR）光谱和纳米滴定（NanoDrop）测量，对配体与 UCNPs 的共轭进行了表征。在乳腺癌 MCF-7 细胞系上对纳米颗粒的细胞吸收进行了研究。结果表明，叶酸和 RGDK 功能化 UCNPs 的细胞摄取率明显更高，这清楚地表明 UCNPs 的特异性靶向在较低能带区域提供了更好的亚细胞成像质量。

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来源期刊

Journal of Nanoparticle Research 工程技术-材料科学：综合

CiteScore

4.40

自引率

4.00%

发文量

198

审稿时长

3.9 months

期刊介绍： The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size. Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology. The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.