Microfluidic paper-based chemiluminescence sensing platform based on functionalized CaCO3 for time-resolved multiplex detection of avian influenza virus biomarkers

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Analytical biochemistry Pub Date : 2024-06-04 DOI:10.1016/j.ab.2024.115583

Yafei Tian, Yujiao Zhang, Xueyun Lu, Dan Xiao, Cuisong Zhou

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Abstract

Multiplex detection can enhance diagnostic precision and improve diagnostic efficiency, providing important assistance for epidemiological investigation and epidemic prevention. There is a great need for multi-detection sensing platforms to accurately diagnose diseases. Herein, we reported a μPAD-based chemiluminescence (CL) assay for ultrasensitive multiplex detection of AIV biomarkers, based on three DNAzyme/Lum/PEI/CaCO₃. Three time-resolved CL signals were sequentially generated with detection limits of 0.32, 0.34, and 0.29 pM for H1N1, H7N9, and H5N1, respectively, and with excellent selectivity against interfering DNA. The recovery test in human serum displayed satisfactory analysis capabilities for complex biological samples. The μPAD-based CL assay achieved multiplex detection within 70 s, with a high time resolution of 20 s. The proposed strategy has the advantages of low cost, high sensitivity, good selectivity, and wide time resolution, the μPAD-based CL assay has shown great potential in the early and accurate diagnosis of diseases.

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基于功能化 CaCO3 的微流纸基化学发光传感平台，用于禽流感病毒生物标记物的时间分辨多重检测。

多重检测可以提高诊断精度和诊断效率，为流行病学调查和防疫提供重要帮助。准确诊断疾病亟需多重检测传感平台。在此，我们报道了一种基于μPAD的化学发光（CL）检测方法，该方法以DNAzyme/Lum/PEI/CaCO3三种化学发光为基础，用于超灵敏地多重检测AIV生物标记物。连续产生的三个时间分辨 CL 信号对 H1N1、H7N9 和 H5N1 的检测限分别为 0.32、0.34 和 0.29 pM，并且对干扰 DNA 具有极佳的选择性。在人血清中进行的回收试验显示出了令人满意的复杂生物样本分析能力。所提出的策略具有成本低、灵敏度高、选择性好、时间分辨率宽等优点，基于 μPAD 的 CL 检测在疾病的早期准确诊断方面具有巨大潜力。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.