Efektifitas Perasan Kulit Mangis Sebagai Pengganti Eosin 2% Pada Pemeriksaan Telur Cacing

E. Febrianti, P. Mulia, Trivini Valencia
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Abstract

Examination of Intestinal Nematode worm eggs is one of the practicum subjects in the Basic Science of Nursing course at the Biomedical Laboratory of the Faculty of Nursing, University of Riau. Eosin 2% is a stain used in the examination of Intestinal Nematode worm eggs. However, the availability of 2% eosin cannot be sufficient to facilitate every student. This is because the procurement of consumables in the laboratory is very limited. An alternative new coloring method is needed using natural ingredients, one of which is derived from mangosteen peel juice. This study aims to determine the effectiveness of the percentage of mangosteen peel juice that can be used as a substitute for 2% eosin. The research design was an experimental study using a completely randomized design (CRD). The subjects of the study were direct staining of mangosteen peel juice and 2% eosin as a control. The object of research is worm positive feces. As a treatment is mangosteen peel which consists of 2 (two) times the level of treatment, namely mangosteen peel juice 5%, 10%. The results showed that a concentration of 10% mangosteen peel juice was effective in examining worm eggs as a substitute for eosin. Based on the results obtained, it can be concluded that mangosteen peel juice is effective as a substitute for 2% eosin in staining worm eggs, namely at a concentration of 10%.
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山竹果皮汁替代 2% 曙红检测虫卵的有效性
肠线虫虫卵检查是廖内大学护理学院生物医学实验室护理学基础科学课程的实习科目之一。2%曙红是用于检查肠线虫虫卵的染色剂。然而,2% 曙红的供应并不能满足每个学生的需要。这是因为实验室采购的消耗品非常有限。我们需要一种使用天然成分的新着色方法,其中一种天然成分是从山竹果皮汁中提取的。本研究旨在确定可用于替代 2% 曙红的山竹果皮汁的百分比的有效性。研究设计是一项采用完全随机设计(CRD)的实验研究。研究对象是山竹果皮汁的直接染色和作为对照的 2% 曙红。研究对象是蠕虫阳性粪便。作为处理的是山竹果皮,由 2(两)倍的处理水平组成,即山竹果皮汁 5%、10%。结果表明,浓度为 10%的山竹果皮汁可有效代替曙红检查虫卵。根据所获得的结果,可以得出结论:山竹果皮汁可有效替代 2% 的伊红对虫卵进行染色,即浓度为 10%。
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