Preparation and anti-tumor effect in hepatocellular carcinoma treatment of AS1411 aptamer-targeted polyphyllin II-loaded PLGA nanoparticles

IF 6.7 3区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Journal of Science: Advanced Materials and Devices Pub Date : 2024-06-02 DOI:10.1016/j.jsamd.2024.100755
Huating Huang , Aqian Chang , Hulinyue Peng , Jing Liu , Aina Yao , Yidan Ruan , Pingzhi Zhang , Tieshan Wang , Changhai Qu , Xingbin Yin , Jian Ni , Xiaoxv Dong
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Abstract

Polyphyllin II (PPII) has been proven to have significant anti-liver cancer activity, but its application is limited by poor solubility, low bioavailability, and systemic toxicity caused by non-selectivity. To address the above problem, PPII was encapsulated into the Poly (lactic-co-glycolic acid) (PLGA) by precipitation method (PPII-NPs) for hepatocellular carcinoma treatment. Subsequently, Box–Behnken design (BBD) with three variables-three levels (33) was utilized to optimize the PPII-NPs formulation. Under optimal conditions, the drug loading of nanoparticles reached 7.29 ± 0.08% and encapsulation efficiency was 80.98 ± 1.63%. Furthermore, aptamer AS1411 was adopted to enhance the tumor-targeting ability of nanoparticles (Apt/PPII-NPs). The drug loading of Apt/PPII-NPs was 6.25 ± 0.26%, had a spherical shape with a rough surface, a particle size of 252.3 ± 3.6 nm, and showed good slow-release performance and stability. In vitro, assays showed that the targeted modified nanoparticles had significant tumor selectivity and exerted efficient anti-tumor effects by inducing tumor cell apoptosis via the mitochondrial apoptotic pathway and death‐receptor pathway. In vivo, anti-tumor evaluation further demonstrated Apt/PPII-NPs not only effectively inhibited the growth of tumors, but also reduced PPII damage to normal tissues. In summary, this report strongly illustrated the advantages of a targeted nanoparticle platform for providing a solution for the rational application of PPII and improving the therapeutic effect of hepatocellular carcinoma.

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AS1411 aptamer 靶向聚菲林 II 载体 PLGA 纳米粒子的制备及其在肝细胞癌治疗中的抗肿瘤作用
聚菲林 II(PPII)已被证实具有显著的抗肝癌活性,但由于其溶解性差、生物利用度低以及非选择性导致的全身毒性,其应用受到了限制。针对上述问题,研究人员采用沉淀法将 PPII 包裹在聚乳酸(PLGA)中(PPII-NPs),用于治疗肝细胞癌。随后,利用三变量-三水平(33)的方框-贝肯设计(BBD)对 PPII-NPs 配方进行了优化。在最佳条件下,纳米颗粒的载药量达到 7.29 ± 0.08%,封装效率为 80.98 ± 1.63%。此外,还采用了aptamer AS1411来增强纳米颗粒(Apt/PPII-NPs)的肿瘤靶向能力。Apt/PPII-NPs的载药量为6.25 ± 0.26%,呈球形,表面粗糙,粒径为252.3 ± 3.6 nm,具有良好的缓释性能和稳定性。体外实验表明,靶向修饰纳米粒子具有显著的肿瘤选择性,可通过线粒体凋亡途径和死亡受体途径诱导肿瘤细胞凋亡,从而发挥高效的抗肿瘤作用。体内抗肿瘤评估进一步表明,Apt/PPII-NPs 不仅能有效抑制肿瘤生长,还能减少 PPII 对正常组织的损伤。总之,本报告有力地说明了靶向纳米粒子平台的优势,为合理应用 PPII 和提高肝细胞癌的治疗效果提供了解决方案。
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文献相关原料
公司名称产品信息其他信息采购帮参考价格
上海源叶 Coumarin 6
¥36.00~¥95226.00
麦克林 dopamine hydrochloride
¥20.00~¥15115.00
麦克林 Polyvinyl alcohol (PVA)
¥19.00~¥9592.00
上海源叶 Polyphyllin II
¥248.00~¥5230.00
上海源叶 PLGA
来源期刊
Journal of Science: Advanced Materials and Devices
Journal of Science: Advanced Materials and Devices Materials Science-Electronic, Optical and Magnetic Materials
CiteScore
11.90
自引率
2.50%
发文量
88
审稿时长
47 days
期刊介绍: In 1985, the Journal of Science was founded as a platform for publishing national and international research papers across various disciplines, including natural sciences, technology, social sciences, and humanities. Over the years, the journal has experienced remarkable growth in terms of quality, size, and scope. Today, it encompasses a diverse range of publications dedicated to academic research. Considering the rapid expansion of materials science, we are pleased to introduce the Journal of Science: Advanced Materials and Devices. This new addition to our journal series offers researchers an exciting opportunity to publish their work on all aspects of materials science and technology within the esteemed Journal of Science. With this development, we aim to revolutionize the way research in materials science is expressed and organized, further strengthening our commitment to promoting outstanding research across various scientific and technological fields.
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