Galleria mellonella larvae as an alternative model to determine the pathogenicity of avian pathogenic Escherichia coli.

IF 2.5 2区农林科学 Q1 VETERINARY SCIENCES Avian Pathology Pub Date : 2024-06-26 DOI:10.1080/03079457.2024.2365932

Elisar Camilotti, Thales Quedi Furian, Karen Apellanis Borges, Oscar Fernando Ortiz Granados, Gabriela Zottis Chitolina, Thaína de Brites Weber, Daniela Tonini da Rocha, Vladimir Pinheiro do Nascimento, Hamilton Luiz de Souza Moraes, Carlos Tadeu Pippi Salle

引用次数: 0

Abstract

Research highlights: Galleria mellonella larvae are a viable model for determining APEC pathogenicity.Larval disease score is the main variable for determining APEC pathogenicity.Response variables should be evaluated up to 24 h post-inoculation.

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将幼虫作为确定禽类致病性大肠杆菌致病性的替代模型。

禽致病性大肠埃希氏菌（APEC）的致病性可通过一天龄雏鸡的生物模型进行可靠测定。然而，由于公众对在研究中使用脊椎动物的担忧，人们开始探索其他生物模型。人们还研究了其他感染模型，包括灰飞虱幼虫。本研究的目的是评估黑线蝇幼虫是否是评估 APEC 分离物致病性的可行体内模型，并确定该模型的最佳方案。首先，测定了来自一天龄雏鸡的 40 株 APEC 分离物的体内致病性指数（PI）。然后，通过测定半数致死剂量、黑化、细胞毒性试验、总血细胞计数、死亡率、构建存活曲线和幼虫疾病评分，使用相同的分离物来确定确定 G. mellonella 幼虫致病性指数的最佳方案。细胞毒性试验和总血细胞计数不适合用于区分致病性组别。半数致死剂量（LD50）是区分高致病性和低致病性的有用工具，但对于中间致病性则不适用。同样，黑色素化也可作为表征 APEC 分离物致病性的变量，但应与其他变量联系起来。死亡率和生存曲线是描述非致病性和高致病性分离物特征的有用工具。在这项研究中，黑线瘿蚊幼虫是一个可行的模型，幼虫疾病评分是决定黑线瘿蚊模型中 APEC 分离物致病性的主要变量。研究要点黑线瘿蚊幼虫是确定 APEC 致病性的一个可行模型，幼虫疾病评分是确定 APEC 致病性的主要变量，应对接种后 24 小时内的反应变量进行评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Avian Pathology 农林科学-兽医学

CiteScore

4.50

自引率

10.70%

发文量

审稿时长

1 months

期刊介绍： Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.