Lianet Abuin-Denis , Elianne Piloto-Sardiñas , Apolline Maitre , Alejandra Wu-Chuang , Lourdes Mateos-Hernández , Patrícia Gonzaga Paulino , Yamil Bello , Frank Ledesma Bravo , Anays Alvarez Gutierrez , Rafmary Rodríguez Fernández , Alier Fuentes Castillo , Luis Méndez Mellor , Angélique Foucault-Simonin , Dasiel Obregon , Mario Pablo Estrada-García , Alina Rodríguez-Mallon , Alejandro Cabezas-Cruz
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引用次数: 0
Abstract
Understanding the intricate ecological interactions within the microbiome of arthropod vectors is crucial for elucidating disease transmission dynamics and developing effective control strategies. In this study, we investigated the ecological roles of Coxiella-like endosymbiont (CLE) and Anaplasma marginale across larval, nymphal, and adult stages of Rhipicephalus microplus. We hypothesized that CLE would show a stable, nested pattern reflecting co-evolution with the tick host, while A. marginale would exhibit a more dynamic, non-nested pattern influenced by environmental factors and host immune responses. Our findings revealed a stable, nested pattern characteristic of co-evolutionary mutualism for CLE, occurring in all developmental stages of the tick. Conversely, A. marginale exhibited variable occurrence but exerted significant influence on microbial community structure, challenging our initial hypotheses of its non-nested dynamics. Furthermore, in silico removal of both microbes from the co-occurrence networks altered network topology, underscoring their central roles in the R. microplus microbiome. Notably, competitive interactions between CLE and A. marginale were observed in nymphal network, potentially reflecting the impact of CLE on the pathogen transstadial-transmission. These findings shed light on the complex ecological dynamics within tick microbiomes and have implications for disease management strategies.
期刊介绍:
Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.