Decoding changes in tumor-infiltrating leukocytes through dynamic experimental models and single-cell technologies

IF 3.2 4区 医学 Q3 CELL BIOLOGY Immunology & Cell Biology Pub Date : 2024-06-10 DOI:10.1111/imcb.12787
Colin YC Lee, Menna R Clatworthy, David R Withers
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Abstract

The ability to characterize immune cells and explore the molecular interactions that govern their functions has never been greater, fueled in recent years by the revolutionary advance of single-cell analysis platforms. However, precisely how immune cells respond to different stimuli and where differentiation processes and effector functions operate remain incompletely understood. Inferring cellular fate within single-cell transcriptomic analyses is now omnipresent, despite the assumptions typically required in such analyses. Recently developed experimental models support dynamic analyses of the immune response, providing insights into the temporal changes that occur within cells and the tissues in which such transitions occur. Here we will review these approaches and discuss how these can be combined with single-cell technologies to develop a deeper understanding of the immune responses that should support the development of better therapeutic options for patients.

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通过动态实验模型和单细胞技术解码肿瘤浸润白细胞的变化。
近年来,随着单细胞分析平台的革命性进步,描述免疫细胞特征和探索支配其功能的分子相互作用的能力空前提高。然而,人们对免疫细胞如何对不同刺激做出反应,以及分化过程和效应功能在何处运作等问题仍不完全清楚。在单细胞转录组分析中推断细胞命运现在已无处不在,尽管这类分析通常需要一些假设。最近开发的实验模型支持对免疫反应进行动态分析,让人们深入了解细胞内发生的时间变化以及发生这种转变的组织。在此,我们将回顾这些方法,并讨论如何将它们与单细胞技术相结合,以加深对免疫反应的理解,从而为患者开发更好的治疗方案提供支持。
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来源期刊
Immunology & Cell Biology
Immunology & Cell Biology 医学-免疫学
CiteScore
7.50
自引率
2.50%
发文量
98
审稿时长
4-8 weeks
期刊介绍: The Australasian Society for Immunology Incorporated (ASI) was created by the amalgamation in 1991 of the Australian Society for Immunology, formed in 1970, and the New Zealand Society for Immunology, formed in 1975. The aim of the Society is to encourage and support the discipline of immunology in the Australasian region. It is a broadly based Society, embracing clinical and experimental, cellular and molecular immunology in humans and animals. The Society provides a network for the exchange of information and for collaboration within Australia, New Zealand and overseas. ASI members have been prominent in advancing biological and medical research worldwide. We seek to encourage the study of immunology in Australia and New Zealand and are active in introducing young scientists to the discipline.
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