Protective effects of Withania somnifera against cyclophosphamide-induced testicular damage in rats.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI:10.5653/cerm.2023.06415
Mehrana Jafari, Ahmad Akbari, Zeynab Esmailpour, Zahra Nadi, Maryam Baazm
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Abstract

Objective: Cyclophosphamide (CP) is an alkylating agent commonly used in cancer treatment. It is known to have detrimental effects on the reproductive system, including the potential to cause infertility. Recently, herbal remedies have gained traction as a complementary approach to addressing these side effects. In this study, our goal was to investigate whether the aqueous-alcoholic extract of Withania somnifera (WS) could mitigate the adverse impacts of CP on testicular tissue.

Methods: Animals were randomly assigned to one of the following groups: control, WS (500 mg/kg), CP (100 mg/kg), CP+WS pre-treatment, and CP+WS post-treatment. WS was administered orally through gavage for 1 month. We assessed sperm parameters, testicular histopathology, and the expression of the Bax and Bcl2 genes in the experimental groups.

Results: Sperm parameters (including count, viability, and motility), the number of spermatogonia, the seminiferous tubule diameter, and Bcl2 gene expression, significantly decreased after CP injection (p<0.05). Conversely, the number of immotile sperm and Bax gene expression significantly increased (p<0.05). Treatment with WS, especially when administered as a pre-treatment, ameliorated the sperm parameters, histological alterations, and the expression of apoptosis-related genes (p<0.05).

Conclusion: The data suggest that WS may mitigate the detrimental effects of CP on testicular tissue by reducing apoptosis. Consequently, WS has the potential to be used as an adjunctive therapy to reduce the complications associated with CP treatment.

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睡茄对环磷酰胺引起的大鼠睾丸损伤的保护作用
目的:环磷酰胺(CP)是一种常用于癌症治疗的烷化剂。众所周知,它对生殖系统有不利影响,包括可能导致不孕。最近,草药疗法作为解决这些副作用的辅助方法受到了关注。在这项研究中,我们的目标是调查睡茄(WS)的水醇提取物是否能减轻氯化石蜡对睾丸组织的不利影响:动物被随机分为以下几组:对照组、WS(500 毫克/千克)组、CP(100 毫克/千克)组、CP+WS 预处理组和 CP+WS 后处理组。WS通过灌胃口服给药,为期1个月。我们评估了实验组的精子参数、睾丸组织病理学以及 Bax 和 Bcl2 基因的表达:结果:注射 CP 后,精子参数(包括数量、存活率和活力)、精原细胞数量、曲细精管直径和 Bcl2 基因表达均显著下降(pConclusion):数据表明,WS 可通过减少细胞凋亡来减轻 CP 对睾丸组织的不利影响。因此,WS 有可能被用作一种辅助疗法,以减少与 CP 治疗相关的并发症。
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