Enzyme-mediated green synthesis of glycosaminoglycans and catalytic process intensification

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology advances Pub Date : 2024-06-08 DOI:10.1016/j.biotechadv.2024.108394
Jie Zheng , Xiao-jun Lin , Han Xu , Muhammad Sohail , Liang-an Chen , Xing Zhang
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Abstract

Glycosaminoglycans (GAGs) are a family of structurally complex heteropolysaccharides that play pivotal roles in biological functions, including the regulation of cell proliferation, enzyme inhibition, and activation of growth factor receptors. Therefore, the synthesis of GAGs is a hot research topic in drug development. The enzymatic synthesis of GAGs has received widespread attention due to their eco-friendly nature, high regioselectivity, and stereoselectivity. The enhancement of the enzymatic synthesis process is the key to its industrial applications. In this review, we overviewed the construction of more efficient in vitro biomimetic synthesis systems of glycosaminoglycans and presented the different strategies to improve enzyme catalysis, including the combination of chemical and enzymatic methods, solid-phase synthesis, and protein engineering to solve the problems of enzyme stability, separation and purification of the product, preparation of structurally defined sugar chains, etc., and discussed the challenges and opportunities in large-scale green synthesis of GAGs.

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酶介导的糖胺聚糖绿色合成及催化过程强化。
糖胺聚糖(GAGs)是一种结构复杂的杂多糖,在调节细胞增殖、酶抑制和激活生长因子受体等生物功能中发挥着举足轻重的作用。因此,GAGs 的合成是药物开发中的一个热门研究课题。GAGs 的酶法合成因其环保性、高区域选择性和立体选择性而受到广泛关注。加强酶法合成工艺是其工业应用的关键。在这篇综述中,我们概述了更高效的糖胺聚糖体外生物模拟合成体系的构建,介绍了改进酶催化的不同策略,包括化学与酶法结合、固相合成、蛋白质工程等,以解决酶的稳定性、产物的分离纯化、结构明确的糖链的制备等问题,并讨论了大规模绿色合成 GAGs 所面临的挑战和机遇。
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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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