A rapid protocol for inducing acute pancreatitis in zebrafish models

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-06-09 DOI:10.1016/j.cbpc.2024.109958
Kívia Vanessa Gomes Falcão , Rafael David Souto de Azevedo , Luiza Rayanna Amorim de Lima , Ranilson de Souza Bezerra
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Abstract

Acute pancreatitis (AP) is an inflammatory disorder that occurs in the exocrine pancreas associated with tissue injury and necrosis. Experimental models of AP typically involve rodents, such as rats or mice. However, rodents exhibit divergent pathophysiological responses after the establishment of AP between themselves and in comparison, with human. The experiments conducted for this manuscript aimed to standardize a new AP model in zebrafish and validate it. Here, we provide a protocol for inducing AP in zebrafish through intraperitoneal injections of synthetic caerulein. Details are provided for solution preparation, pre-injection procedures, injection technique, and monitoring animal survival. Subsequently, validation was performed through biochemical and histological analyses of pancreatic tissue. The administered dose of caerulein for AP induction was 10 μg/kg applied four times in the intraperitoneal region. The histological validation study demonstrated the presence of necrosis within the first 12 h post-injection, accompanied by an excess of zymogen granules in the extracellular milieu. These observations align with those reported in conventional rodent models. We have standardized and validated the AP model in zebrafish. This model can contribute to preclinical and clinical studies of new drugs for AP treatment. Therefore, this novel model expands the toolkit for exploring faster and more effective preventive and therapeutic strategies for AP.

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在斑马鱼模型中诱导急性胰腺炎的快速方案。
急性胰腺炎(AP)是一种发生在胰腺外分泌系统的炎症性疾病,与组织损伤和坏死有关。急性胰腺炎的实验模型通常涉及啮齿类动物,如大鼠或小鼠。然而,啮齿类动物在发生 AP 后表现出不同的病理生理反应,与人类相比也不尽相同。本手稿的实验旨在将斑马鱼的新 AP 模型标准化,并对其进行验证。在此,我们提供了一种通过腹腔注射合成钙松素诱导斑马鱼 AP 的方案。详细介绍了溶液制备、注射前程序、注射技术和动物存活监测。随后,通过胰腺组织的生化和组织学分析进行了验证。用于诱导AP的caerulein给药剂量为10 μg/kg,腹腔注射4次。组织学验证研究表明,在注射后的最初 12 小时内,胰腺组织出现坏死,同时细胞外环境中的酶原颗粒过多。这些观察结果与传统啮齿动物模型的观察结果一致。我们对斑马鱼 AP 模型进行了标准化和验证。该模型有助于治疗 AP 的新药的临床前和临床研究。因此,这种新型模型扩展了探索更快、更有效的 AP 预防和治疗策略的工具包。
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来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
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