Effects of Cadmium and Nickel Mixtures on Multiple Endpoints of the Microalga Raphidocelis subcapitata

IF 3.6 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Environmental Toxicology and Chemistry Pub Date : 2024-06-12 DOI:10.1002/etc.5927

Larissa Luiza dos Reis, Cínthia Bruno de Abreu, Renan Castelhano Gebara, Giseli Swerts Rocha, Elson Longo, Adrislaine da Silva Mansano, Maria da Graça Gama Melão

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Abstract

It is crucial to investigate the effects of mixtures of contaminants on aquatic organisms, because they reflect what occurs in the environment. Cadmium (Cd) and nickel (Ni) are metals that co-occur in aquatic ecosystems, and information is scarce on their joint toxicity to Chlorophyceae using multiple endpoints. We evaluated the effects of isolated and combined Cd and Ni metals on multiple endpoints of the chlorophycean Raphidocelis subcapitata. The results showed that Cd inhibited cell density, increased reactive oxygen species (ROS) production (up to 308% at 0.075 mg L⁻¹ of Cd), chlorophyll a (Chl a) fluorescence (0.050–0.100 mg L⁻¹ of Cd), cell size (0.025–0.100 mg L⁻¹ of Cd), and cell complexity in all concentrations evaluated. Nickel exposure decreased ROS production by up to 25% at 0.25 mg L⁻¹ of Ni and Chl a fluorescence in all concentrations assessed. Cell density and oxygen-evolving complex (initial fluorescence/variable fluorescence [F₀/F_v]) were only affected at 0.5 mg L⁻¹ of Ni. In terms of algal growth, mixture toxicity showed antagonism at low doses and synergism at high doses, with a dose level change greater than the median inhibitory concentration. The independent action model and dose-level–dependent deviation best fit our data. Cadmium and Ni mixtures resulted in a significant increase in cell size and cell complexity, as well as changes in ROS production and Chl a fluorescence, and they did not affect the photosynthetic parameters. Environ Toxicol Chem 2024;43:1855–1869. © 2024 SETAC

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镉和镍混合物对微藻 Raphidocelis subcapitata 多个终点的影响

研究污染物混合物对水生生物的影响至关重要，因为它们反映了环境中发生的情况。镉（Cd）和镍（Ni）是共同存在于水生生态系统中的金属，而关于它们对叶绿藻的联合毒性的信息却很少。我们评估了镉和镍金属的分离和组合对叶绿藻 Raphidocelis subcapitata 多个终点的影响。结果表明，在所有评估浓度下，镉都会抑制细胞密度、增加活性氧（ROS）的产生（镉含量为 0.075 毫克/升时高达 308%）、叶绿素 a（Chl a）荧光（镉含量为 0.050-0.100 毫克/升时）、细胞大小（镉含量为 0.025-0.100 毫克/升时）和细胞复杂性。当镍浓度为 0.25 mg L-1 时，镍暴露会使 ROS 生成量最多减少 25%；在所有评估浓度下，镍暴露都会使 Chl a 荧光减少。细胞密度和氧演化复合物（初始荧光/可变荧光 [F0/Fv]）仅在镍浓度为 0.5 mg L-1 时受到影响。在藻类生长方面，混合物毒性在低剂量时显示出拮抗作用，在高剂量时显示出协同作用，剂量水平变化大于中位抑制浓度。独立作用模型和剂量水平依赖偏差最适合我们的数据。镉和镍混合物导致细胞体积和细胞复杂性显著增加，ROS产生和Chl a荧光也发生了变化，但它们不影响光合作用参数。环境毒物化学 2024;00:1-15。© 2024 SETAC.

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来源期刊

Environmental Toxicology and Chemistry 环境科学-毒理学

CiteScore

7.40

自引率

9.80%

发文量

265

审稿时长

3.4 months

期刊介绍： The Society of Environmental Toxicology and Chemistry (SETAC) publishes two journals: Environmental Toxicology and Chemistry (ET&C) and Integrated Environmental Assessment and Management (IEAM). Environmental Toxicology and Chemistry is dedicated to furthering scientific knowledge and disseminating information on environmental toxicology and chemistry, including the application of these sciences to risk assessment.[...] Environmental Toxicology and Chemistry is interdisciplinary in scope and integrates the fields of environmental toxicology; environmental, analytical, and molecular chemistry; ecology; physiology; biochemistry; microbiology; genetics; genomics; environmental engineering; chemical, environmental, and biological modeling; epidemiology; and earth sciences. ET&C seeks to publish papers describing original experimental or theoretical work that significantly advances understanding in the area of environmental toxicology, environmental chemistry and hazard/risk assessment. Emphasis is given to papers that enhance capabilities for the prediction, measurement, and assessment of the fate and effects of chemicals in the environment, rather than simply providing additional data. The scientific impact of papers is judged in terms of the breadth and depth of the findings and the expected influence on existing or future scientific practice. Methodological papers must make clear not only how the work differs from existing practice, but the significance of these differences to the field. Site-based research or monitoring must have regional or global implications beyond the particular site, such as evaluating processes, mechanisms, or theory under a natural environmental setting.

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