Internal initiation of reverse transcription in a Penelope-like retrotransposon.

IF 4.7 2区 生物学 Q1 GENETICS & HEREDITY Mobile DNA Pub Date : 2024-06-11 DOI:10.1186/s13100-024-00322-z
Chris J Frangieh, Max E Wilkinson, Daniel Strebinger, Jonathan Strecker, Michelle L Walsh, Guilhem Faure, Irina A Yushenova, Rhiannon K Macrae, Irina R Arkhipova, Feng Zhang
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Abstract

Eukaryotic retroelements are generally divided into two classes: long terminal repeat (LTR) retrotransposons and non-LTR retrotransposons. A third class of eukaryotic retroelement, the Penelope-like elements (PLEs), has been well-characterized bioinformatically, but relatively little is known about the transposition mechanism of these elements. PLEs share some features with the R2 retrotransposon from Bombyx mori, which uses a target-primed reverse transcription (TPRT) mechanism, but their distinct phylogeny suggests PLEs may utilize a novel mechanism of mobilization. Using protein purified from E. coli, we report unique in vitro properties of a PLE from the green anole (Anolis carolinensis), revealing mechanistic aspects not shared by other retrotransposons. We found that reverse transcription is initiated at two adjacent sites within the transposon RNA that is not homologous to the cleaved DNA, a feature that is reflected in the genomic "tail" signature shared between and unique to PLEs. Our results for the first active PLE in vitro provide a starting point for understanding PLE mobilization and biology.

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类 Penelope 逆转录酶的内部反转录起始。
真核逆转录子一般分为两类:长末端重复(LTR)逆转录子和非LTR逆转录子。第三类真核逆转录子--类Penelope元件(PLEs)已经有了很好的生物信息表征,但对这些元件的转座机制却知之甚少。PLEs与来自森双翅目昆虫的R2逆转录转座子有一些共同特征,后者使用的是目标刺激反转录(TPRT)机制,但它们不同的系统发育表明PLEs可能使用了一种新的调动机制。利用从大肠杆菌中纯化的蛋白质,我们报告了绿鼹鼠(Anolis carolinensis)的一种 PLE 的独特体外特性,揭示了其他转座子所不具备的机制方面。我们发现,反转录是在转座子 RNA 的两个相邻位点启动的,而这两个位点与被切割的 DNA 并不同源,这一特征反映在基因组 "尾部 "特征上,这是 PLEs 所共有的,也是 PLEs 所独有的。我们对首个体外活性 PLE 的研究结果为了解 PLE 的动员和生物学提供了一个起点。
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来源期刊
Mobile DNA
Mobile DNA GENETICS & HEREDITY-
CiteScore
8.20
自引率
6.10%
发文量
26
审稿时长
11 weeks
期刊介绍: Mobile DNA is an online, peer-reviewed, open access journal that publishes articles providing novel insights into DNA rearrangements in all organisms, ranging from transposition and other types of recombination mechanisms to patterns and processes of mobile element and host genome evolution. In addition, the journal will consider articles on the utility of mobile genetic elements in biotechnological methods and protocols.
期刊最新文献
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