Rmg10, a Novel Wheat Blast Resistance Gene Derived from Aegilops tauschii.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES Phytopathology Pub Date : 2024-09-01 Epub Date: 2024-09-12 DOI:10.1094/PHYTO-01-24-0018-R

Motohiro Yoshioka, Masahiro Kishii, Pawan Kumar Singh, Yoshihiro Inoue, Trinh Thi Phuong Vy, Yukio Tosa, Soichiro Asuke

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Abstract

Wheat blast, caused by Pyricularia oryzae (syn. Magnaporthe oryzae) pathotype Triticum (MoT), is a devastating disease that can result in up to 100% yield loss in affected fields. To find new resistance genes against wheat blast, we screened 199 accessions of Aegilops tauschii, the D genome progenitor of common wheat (Triticum aestivum), by seedling inoculation assays with Brazilian MoT isolate Br48 and found 14 resistant accessions. A synthetic hexaploid wheat line (Ldn/KU-2097) derived from a cross between the T. turgidum 'Langdon' (Ldn) and resistant A. tauschii accession KU-2097 exhibited resistance in seedlings and spikes against Br48. In an F₂ population derived from 'Chinese Spring' × Ldn/KU-2097, resistant and susceptible individuals segregated in a 3:1 ratio, suggesting that the resistance from KU-2097 is controlled by a single dominant gene. We designated this gene Rmg10. Genetic mapping using an F_2:3 population from the same cross mapped the RMG10 locus to the short arm of chromosome 2D. Rmg10 was ineffective against Bangladesh isolates but effective against Brazilian isolates. Field tests in Bolivia showed increased spike resistance in a synthetic octaploid wheat line produced from a cross between common wheat cultivar 'Gladius' and KU-2097. These results suggest that Rmg10 would be beneficial in farmers' fields in South America.

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Rmg10 是一种源自 Aegilops tauschii 的新型小麦抗瘟基因。

由 Pyricularia oryzae（同 Magnaporthe oryzae）病原型 Triticum（MoT）引起的小麦瘟病是一种毁灭性病害，可导致受害田块减产达 100%。为了寻找新的抗麦穗瘟基因，我们用巴西 MoT 分离物 Br48 通过幼苗接种试验筛选了普通小麦（Triticum aestivum L.）D 基因组祖先 Aegilops tauschii Coss. 的 199 个品系，发现了 14 个抗性品系。由 T. turgidum 栽培品种'Langdon'（Ldn）和抗性 Ae. tauschii 编号 KU-2097 杂交产生的合成六倍体小麦品系（Ldn/KU-2097）在幼苗和穗上表现出对 Br48 的抗性。在由'中国之春'（CS）×Ldn/KU-2097 衍生的 F2 群体中，抗性个体和易感个体的分离比例为 3:1，这表明 KU-2097 的抗性由一个显性基因控制。我们将该基因命名为 Rmg10。利用来自同一杂交的 F2:3 群体进行基因图谱绘制，发现 RMG10 基因座位于染色体 2D 的短臂上。Rmg10 对孟加拉国的分离株无效，但对巴西的分离株有效。玻利维亚的田间试验表明，由普通小麦栽培品种 "Gladius "和 KU-2097 杂交产生的合成八倍体小麦品系的抗穗性增强。这些结果表明，Rmg10 对南美洲农民的田地有益。

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.