PagJAZ5 regulates cambium activity through coordinately modulating cytokinin concentration and signaling in poplar

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-06-14 DOI:10.1111/nph.19912

Meng-Xuan Hu, Wei Guo, Xue-Qin Song, Ying-Li Liu, Yuan Xue, Yuan Cao, Jian-Jun Hu, Meng-Zhu Lu, Shu-Tang Zhao

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Abstract

Wood is resulted from the radial growth paced by the division and differentiation of vascular cambium cells in woody plants, and phytohormones play important roles in cambium activity.
Here, we identified that PagJAZ5, a key negative regulator of jasmonate (JA) signaling, plays important roles in enhancing cambium cell division and differentiation by mediating cytokinin signaling in poplar 84K (Populus alba × Populus glandulosa).
PagJAZ5 is preferentially expressed in developing phloem and cambium, weakly in developing xylem cells. Overexpression (OE) of PagJAZ5m (insensitive to JA) increased cambium activity and xylem differentiation, while jaz mutants showed opposite results. Transcriptome analyses revealed that cytokinin oxidase/dehydrogenase (CKXs) and type-A response regulators (RRs) were downregulated in PagJAZ5m OE plants. The bioactive cytokinins were significantly increased in PagJAZ5m overexpressing plants and decreased in jaz5 mutants, compared with that in 84K plants. The PagJAZ5 directly interact with PagMYC2a/b and PagWOX4b. Further, we found that the PagRR5 is regulated by PagMYC2a and PagWOX4b and involved in the regulation of xylem development.
Our results showed that PagJAZ5 can increase cambium activity and promote xylem differentiation through modulating cytokinin level and type-A RR during wood formation in poplar.

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PagJAZ5 通过协调调节杨树细胞分裂素的浓度和信号传导来调节韧皮部活性。

木材是由木本植物的维管束壁细胞分裂和分化形成的径向生长，而植物激素在壁细胞活动中发挥着重要作用。在这里，我们发现 PagJAZ5 是茉莉酸盐（JA）信号传导的一个关键负调控因子，它通过介导细胞分裂素信号传导在杨树 84K（Populus alba × Populus glandulosa）中对增强韧皮部细胞分裂和分化起着重要作用。PagJAZ5 优先在发育中的韧皮部和骨皮层中表达，在发育中的木质部细胞中表达较弱。PagJAZ5m（对 JA 不敏感）的过表达（OE）增加了木质部的活性和木质部的分化，而 jaz 突变体则表现出相反的结果。转录组分析表明，细胞分裂素氧化酶/脱氢酶（CKXs）和 A 型响应调节因子（RRs）在 PagJAZ5m OE 植物中下调。与84K植株相比，PagJAZ5m过表达植株的生物活性细胞分裂素显著增加，而jaz5突变体的生物活性细胞分裂素显著减少。PagJAZ5与PagMYC2a/b和PagWOX4b直接相互作用。此外，我们还发现 PagRR5 受 PagMYC2a 和 PagWOX4b 的调控，参与木质部发育的调控。我们的研究结果表明，PagJAZ5 能在杨树木质部形成过程中通过调节细胞分裂素水平和 A 型 RR 增加木质部活性并促进木质部分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.