Effects of 10-MDP calcium salt on osteoblasts and fibroblasts.

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Dental Materials Pub Date : 2024-06-13 DOI:10.1016/j.dental.2024.06.015

Lvhui Zhou, Yumin Wu, Zehua Bai, Jingjing Bian, Haifeng Xie, Chen Chen

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引用次数: 0

Abstract

Objective: 10-methacryloyloxidecyl dihydrogen phosphate monomer (10-MDP) is commonly used as a bonding monomer in universal adhesives. Adhesives that contain this monomer can directly contact the surrounding periodontium due to the chemical binding of 10-MDP with hydroxyapatite in hard tissue to form calcium salts. However, the effect of these calcium salts on the periodontium in the case of subgingival fillings remains poorly understood. The objective of this study was to investigate effects of 10-MDP calcium salts on osteoblasts and fibroblasts in the periodontal tissues.

Methods: This study investigated the effects of different concentrations of 10-MDP calcium salts on the migration, proliferation, and differentiation of osteoblasts (MC3T3-E1) and fibroblasts (L929); additionally, the effect on apoptosis and matrix metalloproteinases (MMPs) expression in these cells was evaluated. Cell proliferation assay, alkaline phosphatase (ALP) activity assay, Western blotting, and quantitative real-time polymerase chain reaction were performed to determine the effects.

Results: The 10-MDP calcium salts (within a concentration of 0.5 mg/mL) showed no cytotoxicity and did not seem to influence the apoptosis, mitochondrial membrane potential, and reactive oxygen species (ROS) levels in the cells. However, they had an inhibitory effect on the secretion of MMP2 and MMP9 in the osteoblasts and fibroblasts. The ALP activity assay and Alizarin Red staining did not reveal any significant effects of the 10-MDP calcium salts on osteoblast differentiation.

Significance: These results suggest that applying 10-MDP-containing adhesives to subgingival fillings may be safe and beneficial for the periodontal tissues.

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10-MDP 钙盐对成骨细胞和成纤维细胞的影响

目的：10-甲基丙烯酰氧癸基磷酸二氢酯单体（10-MDP）通常用作通用粘合剂的粘合单体。由于 10-MDP 与硬组织中的羟基磷灰石化学结合形成钙盐，因此含有这种单体的粘合剂可直接接触周围的牙周。然而，这些钙盐对龈下充填物牙周的影响仍然知之甚少。本研究的目的是调查 10-MDP 钙盐对牙周组织中成骨细胞和成纤维细胞的影响：本研究调查了不同浓度的 10-MDP 钙盐对成骨细胞（MC3T3-E1）和成纤维细胞（L929）迁移、增殖和分化的影响；此外，还评估了对这些细胞凋亡和基质金属蛋白酶（MMPs）表达的影响。为了确定这些影响，还进行了细胞增殖测定、碱性磷酸酶（ALP）活性测定、Western 印迹和实时定量聚合酶链反应：10-MDP 钙盐（浓度在 0.5 毫克/毫升以内）没有细胞毒性，似乎也不会影响细胞凋亡、线粒体膜电位和活性氧（ROS）水平。不过，它们对成骨细胞和成纤维细胞中 MMP2 和 MMP9 的分泌有抑制作用。ALP 活性测定和茜素红染色并未显示 10-MDP 钙盐对成骨细胞分化有任何显著影响：这些结果表明，在龈下填料中使用含 10-MDP 的粘合剂可能是安全的，而且对牙周组织有益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.