Heterotopic ovarian allotransplantation in a caprine model: Effects of implant site on morphological parameters

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Reproduction Science Pub Date : 2024-06-09 DOI:10.1016/j.anireprosci.2024.107509

Antonio Renilson Sousa Vieira , Leda Maria Costa Pereira Bersano , Fabiana Aparecida Santilli Brandão , Celso Henrique Souza Costa Barros , Francisco Carlos de Sousa , Ana Luiza de Sousa Rodrigues , Benner Geraldo Alves , Francisco Denilson Rodrigues Gomes , Ana Paula Ribeiro Rodrigues , Dárcio Ítalo Alves Teixeira

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Abstract

This study aimed to investigate a new implantation site (intra-auricular subcutaneous - IA) compared to intramuscular (IM) in the cervical portion (cervical splenius muscle) of the neck for ovarian transplantation in goats. Morphological aspects of the implant, follicular activation and morphology, and type I and III collagen deposits of the transplanted tissue were evaluated. Four fragments of the ovarian cortex were allotransplanted at the IA and IM sites in all goat recipients and recovered 7 (IA-7; IM-7) or 15 (IA-15; IM-15) days later and submitted to histological analysis. Two fragments/animal were separated for the fresh control (FC) group. There was a higher percentage of normal and developing primordial follicles at the IA-7 site (P < 0.05) compared to the other treatments, with similar values to the fresh control. Type I and III collagen fibers differed between the groups (P < 0.05), showing a considerable decrease in type I collagen fibers at the IA-7 site compared to the FC. However, the IM-7 and IA-15 sites showed higher values of type I collagen fibers, showing similarity to the FC. Therefore, we conclude that the IA site in goats is an effective site for ovarian tissue transplantation, as it is easily accessible, low invasive and has presented satisfactory rates of morphology and follicular activation.

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山羊模型中的异位卵巢异位移植：植入部位对形态参数的影响

本研究旨在探讨一种新的植入部位（耳内皮下 - IA），与颈部（颈脾肌）肌肉注射（IM）相比，用于山羊卵巢移植。对植入物的形态、卵泡活化和形态以及移植组织的 I 型和 III 型胶原沉积进行了评估。在所有山羊的IA和IM部位异体移植了四个卵巢皮质片段，7（IA-7；IM-7）或15（IA-15；IM-15）天后取出并进行组织学分析。新鲜对照（FC）组每只动物分离出两个片段。与其他处理相比，IA-7部位正常和发育中原始卵泡的百分比更高（P <0.05），与新鲜对照组的数值相似。各组之间的 I 型和 III 型胶原纤维存在差异（P < 0.05），与 FC 相比，IA-7 处的 I 型胶原纤维明显减少。然而，IM-7 和 IA-15 位点的 I 型胶原纤维值较高，显示与 FC 相似。因此，我们得出结论，山羊的 IA 位点是卵巢组织移植的有效位点，因为该位点容易接近、创伤小，而且形态和卵泡活化率令人满意。

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.