Animal Reproduction Science最新文献

The use of wheat grass juice as a promising functional feed additive for enhancing reproductive performance and larvae quality of sterlet (Acipenser ruthenus)

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-15 DOI: 10.1016/j.anireprosci.2025.107796

C.-A. Barbacariu , G. Dumitru , L. Dîrvariu , D.A. Şerban , I. Dincheva , E. Todirascu-Ciornea , M. Burducea

The optimization of broodstock nutrition remains a critical challenge in sturgeon aquaculture. This study investigated the effects of wheatgrass juice (WGJ) supplementation of sterlet (Acipenser ruthenus) broodstock on reproductive performance, larval quality, hematological profile and oxidative status. Female sterlets were randomly assigned to three groups: control diet (V1), WGJ-supplemented diet (V2, 4 % inclusion), both (V1 and V2) hormonally stimulated and saline solution (V3) without hormonal stimulation. WGJ's chemical composition revealed substantial levels of bioactive compounds, including chlorophyll (a: 0.936 ± 0.01, b: 0.329 ± 0.01 mg/mL fw), carotenoids (0.167 ± 0.01 mg/mL fw), proteins (1.205 ± 0.04 mg PSB/mL fw), flavonoids (7.381 ± 0.31 mg/mL fw), and polyphenols (2.963 ± 0.05 mg/mL fw). WGJ supplementation significantly enhanced reproductive parameters, with V2 showing higher egg production (0.23 ± 0.03 kg vs. 0.13 ± 0.01 kg) and gonadosomatic index (14.48 ± 1.73 vs. 8.05 ± 0.51) compared to V1. Larvae from WGJ-supplemented females exhibited significantly improved length (10.9 ± 0.3 cm vs. 9.8 ± 0.14 cm, p < 0.001) and weight parameters. Analysis of oxidative stress markers revealed tissue-specific modulation of antioxidant systems, with WGJ supplementation reducing malondialdehyde levels across tissues (muscle: 1.061 ± 0.075 vs. 1.888 ± 0.105 nM/mg protein, p < 0.0001) and enhancing antioxidant enzyme activities. Hematological parameters remained within physiological ranges, with reduced neutrophil counts in the WGJ group suggesting potential immunomodulatory effects. These findings establish WGJ as a promising functional feed additive for enhancing reproductive performance in sterlet aquaculture, while maintaining optimal physiological status through improved antioxidant defense mechanisms.

{"title":"The use of wheat grass juice as a promising functional feed additive for enhancing reproductive performance and larvae quality of sterlet (Acipenser ruthenus)","authors":"C.-A. Barbacariu , G. Dumitru , L. Dîrvariu , D.A. Şerban , I. Dincheva , E. Todirascu-Ciornea , M. Burducea","doi":"10.1016/j.anireprosci.2025.107796","DOIUrl":"10.1016/j.anireprosci.2025.107796","url":null,"abstract":"<div><div>The optimization of broodstock nutrition remains a critical challenge in sturgeon aquaculture. This study investigated the effects of wheatgrass juice (WGJ) supplementation of sterlet (<em>Acipenser ruthenus</em>) broodstock on reproductive performance, larval quality, hematological profile and oxidative status. Female sterlets were randomly assigned to three groups: control diet (V1), WGJ-supplemented diet (V2, 4 % inclusion), both (V1 and V2) hormonally stimulated and saline solution (V3) without hormonal stimulation. WGJ's chemical composition revealed substantial levels of bioactive compounds, including chlorophyll (a: 0.936 ± 0.01, b: 0.329 ± 0.01 mg/mL fw), carotenoids (0.167 ± 0.01 mg/mL fw), proteins (1.205 ± 0.04 mg PSB/mL fw), flavonoids (7.381 ± 0.31 mg/mL fw), and polyphenols (2.963 ± 0.05 mg/mL fw). WGJ supplementation significantly enhanced reproductive parameters, with V2 showing higher egg production (0.23 ± 0.03 kg vs. 0.13 ± 0.01 kg) and gonadosomatic index (14.48 ± 1.73 vs. 8.05 ± 0.51) compared to V1. Larvae from WGJ-supplemented females exhibited significantly improved length (10.9 ± 0.3 cm vs. 9.8 ± 0.14 cm, p < 0.001) and weight parameters. Analysis of oxidative stress markers revealed tissue-specific modulation of antioxidant systems, with WGJ supplementation reducing malondialdehyde levels across tissues (muscle: 1.061 ± 0.075 vs. 1.888 ± 0.105 nM/mg protein, p < 0.0001) and enhancing antioxidant enzyme activities. Hematological parameters remained within physiological ranges, with reduced neutrophil counts in the WGJ group suggesting potential immunomodulatory effects. These findings establish WGJ as a promising functional feed additive for enhancing reproductive performance in sterlet aquaculture, while maintaining optimal physiological status through improved antioxidant defense mechanisms.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107796"},"PeriodicalIF":2.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nicotinamide mononucleotide enhances porcine sperm quality by activating the SIRT3-SOD2/ROS pathway and promoting oxidative phosphorylation

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-15 DOI: 10.1016/j.anireprosci.2025.107797

Haize Zhang, Xue Qin, Nataraj Bojan, Chaoyue Cao, Jiawen Chai, Weijun Pang

Sperm quality is crucial for animal reproduction but is often deteriorates due to oxidative stress. Preserving functionality during storage by protecting it from oxidative stress is crucial for enhancing the success of assisted reproductive technologies and maintaining fertility. Nicotinamide mononucleotide (NMN), a precursor of Nicotinamide adenine dinucleotide (NAD⁺), acts as a potent antioxidant. However, its role and regulatory mechanism in porcine sperm liquid preservation in vitro are remains unclear. In this study we demonstrated that NMN supplementation in the semen diluent significantly improved porcine sperm quality during storage at 17 °C by increasing NAD⁺ concentrations and decreasing the reactive oxygen species (ROS) concentrations in sperm. Subsequent analysis revealed that NMN, upon conversion to NAD⁺ within sperm, exerted antioxidant effect by upregulating the mitochondrial protein sirtuin 3 (SIRT3), resulting in the downregulation of superoxide dismutase 2 (SOD2) acetylation. This pathway mitigated oxidative stress. Additionally, NMN also enhanced sperm oxidative phosphorylation (OXPHOS) thereby increase adenosine triphosphate (ATP) production concentrations via activating SIRT3, without affecting sperm glycolysis. Furthermore, the semen diluent supplemented with 50 μg/mL NMN resulted in improved reproductive outcomes, including the birth of healthier piglets and a reduction in stillbirths rates following artificial insemination (AI). Taken together, NMN improves porcine sperm quality through SIRT3-activating the SOD2/ROS axis and oxidative phosphorylation. Our findings advance understanding of sperm physiology and metabolism and provide a new avenue promising approach for developing innovative semen diluents for liquid preservation in animal reproduction biology in vitro.

{"title":"Nicotinamide mononucleotide enhances porcine sperm quality by activating the SIRT3-SOD2/ROS pathway and promoting oxidative phosphorylation","authors":"Haize Zhang, Xue Qin, Nataraj Bojan, Chaoyue Cao, Jiawen Chai, Weijun Pang","doi":"10.1016/j.anireprosci.2025.107797","DOIUrl":"10.1016/j.anireprosci.2025.107797","url":null,"abstract":"<div><div>Sperm quality is crucial for animal reproduction but is often deteriorates due to oxidative stress. Preserving functionality during storage by protecting it from oxidative stress is crucial for enhancing the success of assisted reproductive technologies and maintaining fertility. Nicotinamide mononucleotide (<strong>NMN</strong>), a precursor of Nicotinamide adenine dinucleotide (<strong>NAD</strong><sup><strong>+</strong></sup>), acts as a potent antioxidant. However, its role and regulatory mechanism in porcine sperm liquid preservation <em>in vitro</em> are remains unclear. In this study we demonstrated that NMN supplementation in the semen diluent significantly improved porcine sperm quality during storage at 17 °C by increasing NAD<sup>+</sup> concentrations and decreasing the reactive oxygen species (<strong>ROS</strong>) concentrations in sperm. Subsequent analysis revealed that NMN, upon conversion to NAD<sup>+</sup> within sperm, exerted antioxidant effect by upregulating the mitochondrial protein sirtuin 3 (<strong>SIRT3</strong>), resulting in the downregulation of superoxide dismutase 2 (<strong>SOD2</strong>) acetylation. This pathway mitigated oxidative stress. Additionally, NMN also enhanced sperm oxidative phosphorylation (<strong>OXPHOS</strong>) thereby increase adenosine triphosphate (<strong>ATP</strong>) production concentrations via activating SIRT3, without affecting sperm glycolysis. Furthermore, the semen diluent supplemented with 50 μg/mL NMN resulted in improved reproductive outcomes, including the birth of healthier piglets and a reduction in stillbirths rates following artificial insemination (<strong>AI</strong>). Taken together, NMN improves porcine sperm quality through SIRT3-activating the SOD2/ROS axis and oxidative phosphorylation. Our findings advance understanding of sperm physiology and metabolism and provide a new avenue promising approach for developing innovative semen diluents for liquid preservation in animal reproduction biology <em>in vitro.</em></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107797"},"PeriodicalIF":2.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of dietary supplementation of Bacillus subtilis QST 713 on constipation, reproductive performance and offspring growth performance of sows

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-12 DOI: 10.1016/j.anireprosci.2025.107785

Fuyong Li , Deyuan Wu , Kaidi Ma , Tanghong Wei , Junyi Wu , Shijian Zhou , Shizhe Xiang , Zhenpeng Zhu , Xiandong Zhang , Chengquan Tan , Hefeng Luo , Jinping Deng

The experiment aimed to investigate the effects of dietary supplementation with Bacillus subtilis QST 713 (BS) on the reproductive performance, perinatal constipation, and offspring growth performance of sows. 78 multiparous Landrace-Yorkshire sows with same parity and backfat thickness were divided into 2 groups: a basal diet group (CON) and a basal diet supplemented with 1.5 × 10⁹ CFU/kg BS. The experiment was conducted from day 85 of gestation to day 21 of lactation. Reproductive performance, lactation performance, and perinatal fecal scores of sows were recorded. Samples were collected for subsequent analysis, including the feces of sows on day 110 of gestation, colostrum within 2 hours of parturition, milk on day 21 of lactation and feces from sows on day 21 of lactation. The results showed that, compared with the CON group, the BS group significantly shortened the duration of parturition (P < 0.01) and the average birth interval of piglets (P < 0.05), reduced stillbirth rate (P < 0.05) and invalid piglet rate (P < 0.05). Additionally, the BS group increased the content of protein (P < 0.05) and level of IgM in colostrum (P < 0.05), as well as the average daily gain (P < 0.05) of weaned piglets. The BS group increased the perinatal fecal score (P < 0.05) of sows, decreased the incidence of post-partum constipation (P < 0.05), and increased the relative abundance of Lactobacillus (P < 0.05) in feces on day 110 of gestation. In summary, a maternal diet supplemented with BS reduced the stillbirth rate by shortening the duration of parturition, alleviated perinatal constipation, and improved intestinal microbiota, meanwhile improving the growth performance of piglets by increasing nutrients and immunoglobulins in colostrum.

{"title":"Effect of dietary supplementation of Bacillus subtilis QST 713 on constipation, reproductive performance and offspring growth performance of sows","authors":"Fuyong Li , Deyuan Wu , Kaidi Ma , Tanghong Wei , Junyi Wu , Shijian Zhou , Shizhe Xiang , Zhenpeng Zhu , Xiandong Zhang , Chengquan Tan , Hefeng Luo , Jinping Deng","doi":"10.1016/j.anireprosci.2025.107785","DOIUrl":"10.1016/j.anireprosci.2025.107785","url":null,"abstract":"<div><div>The experiment aimed to investigate the effects of dietary supplementation with <em>Bacillus subtilis</em> QST 713 (BS) on the reproductive performance, perinatal constipation, and offspring growth performance of sows. 78 multiparous Landrace-Yorkshire sows with same parity and backfat thickness were divided into 2 groups: a basal diet group (CON) and a basal diet supplemented with 1.5 × 10<sup>9</sup> CFU/kg BS. The experiment was conducted from day 85 of gestation to day 21 of lactation. Reproductive performance, lactation performance, and perinatal fecal scores of sows were recorded. Samples were collected for subsequent analysis, including the feces of sows on day 110 of gestation, colostrum within 2 hours of parturition, milk on day 21 of lactation and feces from sows on day 21 of lactation. The results showed that, compared with the CON group, the BS group significantly shortened the duration of parturition (<em>P</em> < 0.01) and the average birth interval of piglets (<em>P</em> < 0.05), reduced stillbirth rate (<em>P</em> < 0.05) and invalid piglet rate (<em>P</em> < 0.05). Additionally, the BS group increased the content of protein (<em>P</em> < 0.05) and level of IgM in colostrum (<em>P</em> < 0.05), as well as the average daily gain (<em>P</em> < 0.05) of weaned piglets. The BS group increased the perinatal fecal score (<em>P</em> < 0.05) of sows, decreased the incidence of post-partum constipation (<em>P</em> < 0.05), and increased the relative abundance of <em>Lactobacillus</em> (<em>P</em> < 0.05) in feces on day 110 of gestation. In summary, a maternal diet supplemented with BS reduced the stillbirth rate by shortening the duration of parturition, alleviated perinatal constipation, and improved intestinal microbiota, meanwhile improving the growth performance of piglets by increasing nutrients and immunoglobulins in colostrum.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107785"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the mechanism of sperm cell aging – Functional, structural, and kinematic characteristics in fresh semen of mature and aged dogs

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-11 DOI: 10.1016/j.anireprosci.2025.107798

Kenneth Owoicho Abah , Zuzanna Ligocka-Kowalczyk , Agnieszka Partyka , Sylwia Prochowska , Alain Fontbonne , Wojciech Nizanski

This study aimed to evaluate fresh semen from mature and senile dogs, identify differences in sperm parameters, and determine the correlations between sperm variables and semen quality. Forty dogs were divided into two groups based on age: mature, aged 2–4 years (n = 20); and senile, aged ≥ 9 years (n = 20). Two or three ejaculates, totaling 107 samples, were collected from each dog and evaluated for various parameters including motility and sperm movement kinematics, morphology and viability, membrane lipid disorder, DNA integrity, membrane integrity, mitochondrial potential, seminal plasma total antioxidant capacity, apoptotic-like changes, and proAKAP4 protein levels. The results showed significantly lower values for progressive motility, average path velocity, straight-line velocity, and curvilinear velocity in senile dogs than in mature dogs (P < 0.001). At the same time, the proportions of slow motile and static sperm were higher in senile dogs (P < 0.05). The semen of senile dogs was characterized by a higher percentage of apoptotic sperm, lower mitochondrial potential, higher levels of proAKAP4, and a higher percentage of defective spermatozoa (P < 0.01). The two groups had no significant differences in DNA fragmentation, membrane lipid disorder, and total antioxidant capacity in the seminal plasma. In conclusion, advancing male age in dogs is associated with the expression of early apoptotic-like changes, as evidenced by significantly increased permeability of the sperm membrane to YO-PRO-1 and low mitochondrial potential. These apoptotic markers were correlated with reduced sperm quality parameters, highlighting the impact of aging on sperm functionality.

{"title":"Deciphering the mechanism of sperm cell aging – Functional, structural, and kinematic characteristics in fresh semen of mature and aged dogs","authors":"Kenneth Owoicho Abah , Zuzanna Ligocka-Kowalczyk , Agnieszka Partyka , Sylwia Prochowska , Alain Fontbonne , Wojciech Nizanski","doi":"10.1016/j.anireprosci.2025.107798","DOIUrl":"10.1016/j.anireprosci.2025.107798","url":null,"abstract":"<div><div>This study aimed to evaluate fresh semen from mature and senile dogs, identify differences in sperm parameters, and determine the correlations between sperm variables and semen quality. Forty dogs were divided into two groups based on age: mature, aged 2–4 years (<em>n</em> = 20); and senile, aged ≥ 9 years (<em>n</em> = 20). Two or three ejaculates, totaling 107 samples, were collected from each dog and evaluated for various parameters including motility and sperm movement kinematics, morphology and viability, membrane lipid disorder, DNA integrity, membrane integrity, mitochondrial potential, seminal plasma total antioxidant capacity, apoptotic-like changes, and proAKAP4 protein levels. The results showed significantly lower values for progressive motility, average path velocity, straight-line velocity, and curvilinear velocity in senile dogs than in mature dogs (<em>P</em> < 0.001). At the same time, the proportions of slow motile and static sperm were higher in senile dogs (<em>P</em> < 0.05). The semen of senile dogs was characterized by a higher percentage of apoptotic sperm, lower mitochondrial potential, higher levels of proAKAP4, and a higher percentage of defective spermatozoa (<em>P</em> < 0.01). The two groups had no significant differences in DNA fragmentation, membrane lipid disorder, and total antioxidant capacity in the seminal plasma. In conclusion, advancing male age in dogs is associated with the expression of early apoptotic-like changes, as evidenced by significantly increased permeability of the sperm membrane to YO-PRO-1 and low mitochondrial potential. These apoptotic markers were correlated with reduced sperm quality parameters, highlighting the impact of aging on sperm functionality.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107798"},"PeriodicalIF":2.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interplay between thyroxine and cortisol in zebrafish: Effects on growth, physiology, and reproduction

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-09 DOI: 10.1016/j.anireprosci.2025.107795

Hamed Abdollahpour, Naghmeh Jafari Pastaki, Bahram Falahatkar

In the current study, the role of thyroxine (T4) and cortisol (CO) on growth, reproduction, and whole-body hormone levels in male and female zebrafish was assayed. Fifty-seven days post-fertilization, zebrafish [132 females; initial weight: 0.3 ± 0.0 g and 132 males (initial weight: 0.2 ± 0.0 g)] were fed with diets including 10 mg T4 kg feed⁻¹ (T4), 10 mg CO kg feed⁻¹ (CO), 10 mg T4 kg feed⁻¹ + 10 mg CO kg feed⁻¹ (T4 + CO), and a control group (without hormones) for 28 days. At the end of the experiment, growth performance, feed utilization, whole-body hormone levels, gonadal development, and spawning performance were determined. The growth performance was improved following T4 administration in both males and females. Viscerosomatic and gonadosomatic indices in females were significantly enhanced in the T4 group compared with the other groups. Regarding oocyte developmental stages, a lower number of oocytes at final maturation were found in the CO group compared with the other treatments. Oocyte diameter was significantly increased in T4 compared with the other groups. Whole-body CO levels in both males and females were increased and decreased in CO and T4 treatments, respectively. Moreover, whole-body thyroid hormone levels in both sexes were higher in T4 group than the other groups. The hatching rate and survival rate at three days post-hatch were increased in the T4 group. Overall, the findings of this study underscore the stimulatory role of T4 in enhancing the physiological performance of zebrafish, leading to improved growth and increased reproductive output in both males and females.

{"title":"The interplay between thyroxine and cortisol in zebrafish: Effects on growth, physiology, and reproduction","authors":"Hamed Abdollahpour, Naghmeh Jafari Pastaki, Bahram Falahatkar","doi":"10.1016/j.anireprosci.2025.107795","DOIUrl":"10.1016/j.anireprosci.2025.107795","url":null,"abstract":"<div><div>In the current study, the role of thyroxine (T4) and cortisol (CO) on growth, reproduction, and whole-body hormone levels in male and female zebrafish was assayed. Fifty-seven days post-fertilization, zebrafish [132 females; initial weight: 0.3 ± 0.0 g and 132 males (initial weight: 0.2 ± 0.0 g)] were fed with diets including 10 mg T4 kg feed<sup>−1</sup> (T4), 10 mg CO kg feed<sup>−1</sup> (CO), 10 mg T4 kg feed<sup>−1</sup> + 10 mg CO kg feed<sup>−1</sup> (T4 + CO), and a control group (without hormones) for 28 days. At the end of the experiment, growth performance, feed utilization, whole-body hormone levels, gonadal development, and spawning performance were determined. The growth performance was improved following T4 administration in both males and females. Viscerosomatic and gonadosomatic indices in females were significantly enhanced in the T4 group compared with the other groups. Regarding oocyte developmental stages, a lower number of oocytes at final maturation were found in the CO group compared with the other treatments. Oocyte diameter was significantly increased in T4 compared with the other groups. Whole-body CO levels in both males and females were increased and decreased in CO and T4 treatments, respectively. Moreover, whole-body thyroid hormone levels in both sexes were higher in T4 group than the other groups. The hatching rate and survival rate at three days post-hatch were increased in the T4 group. Overall, the findings of this study underscore the stimulatory role of T4 in enhancing the physiological performance of zebrafish, leading to improved growth and increased reproductive output in both males and females.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107795"},"PeriodicalIF":2.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De-SUMO1ylated hexokinase 1 selectively degraded by ubiquitin-proteasome pathway promotes boar sperm capacitation in vitro

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-08 DOI: 10.1016/j.anireprosci.2025.107794

Shunfa Yao , Qinglong Xu , Yanqiu Lv, Yuyang Zhang, Hao Li, Junzheng Zhang, Zhiwei Yao, Yang An, Xuan Chen, Yi Jin

This study investigates the effect of de-SUMOylated hexokinase 1 (HK1) degradation on sperm capacitation in vitro. Sperm from adult Landrace pigs was divided into three groups: non-capacitated group, capacitated group, and 16 mM 2-deoxy-D-glucose (2-DG) group. Immunoprecipitation was performed to detect the binding levels of HK1 and small ubiquitin-like modifier 1 (SUMO1) before and after capacitation. Western blotting was used to assess the degradation pathways of HK1 and tyrosine phosphorylation levels after capacitation. Immunofluorescence was employed to localize HK1 and SUMO1 in sperm. A kit was used to measure pyruvate and adenosine triphosphate (ATP) content. Fluorescent staining was used to detect reactive oxygen species (ROS) levels, zinc (Zn²⁺) levels, calcium (Ca²⁺) levels, and mitochondrial membrane potential (MMP) in sperm. Hoechst 33342 staining was used to visualize sperm nuclei and assess sperm-oocyte adhesion rates. The results showed that inhibition of HK1 activity significantly reduced pyruvate and ATP content, sperm capacitation markers (tyrosine phosphorylation, Zn²⁺), MMP, and sperm adhesion rates (P < 0.05), while significantly increasing ROS levels in capacitated sperm (P < 0.01). Immunoprecipitation and Western blotting results indicated that HK1 undergoes de-SUMOylation during capacitation and is selectively degraded via the ubiquitin-proteasome pathway (P < 0.01). Our findings suggest that de-SUMOylated HK1 activates the glycolytic pathway through ubiquitin-proteasome pathway (UPP) degradation, thereby promoting sperm capacitation. These results provide new theoretical insights into the molecular mechanisms of sperm capacitation.

{"title":"De-SUMO1ylated hexokinase 1 selectively degraded by ubiquitin-proteasome pathway promotes boar sperm capacitation in vitro","authors":"Shunfa Yao , Qinglong Xu , Yanqiu Lv, Yuyang Zhang, Hao Li, Junzheng Zhang, Zhiwei Yao, Yang An, Xuan Chen, Yi Jin","doi":"10.1016/j.anireprosci.2025.107794","DOIUrl":"10.1016/j.anireprosci.2025.107794","url":null,"abstract":"<div><div>This study investigates the effect of de-SUMOylated hexokinase 1 (HK1) degradation on sperm capacitation in vitro. Sperm from adult Landrace pigs was divided into three groups: non-capacitated group, capacitated group, and 16 mM 2-deoxy-D-glucose (2-DG) group. Immunoprecipitation was performed to detect the binding levels of HK1 and small ubiquitin-like modifier 1 (SUMO1) before and after capacitation. Western blotting was used to assess the degradation pathways of HK1 and tyrosine phosphorylation levels after capacitation. Immunofluorescence was employed to localize HK1 and SUMO1 in sperm. A kit was used to measure pyruvate and adenosine triphosphate (ATP) content. Fluorescent staining was used to detect reactive oxygen species (ROS) levels, zinc (Zn²⁺) levels, calcium (Ca²⁺) levels, and mitochondrial membrane potential (MMP) in sperm. Hoechst 33342 staining was used to visualize sperm nuclei and assess sperm-oocyte adhesion rates. The results showed that inhibition of HK1 activity significantly reduced pyruvate and ATP content, sperm capacitation markers (tyrosine phosphorylation, Zn²⁺), MMP, and sperm adhesion rates (<em>P</em> < 0.05), while significantly increasing ROS levels in capacitated sperm (<em>P</em> < 0.01). Immunoprecipitation and Western blotting results indicated that HK1 undergoes de-SUMOylation during capacitation and is selectively degraded via the ubiquitin-proteasome pathway (<em>P</em> < 0.01). Our findings suggest that de-SUMOylated HK1 activates the glycolytic pathway through ubiquitin-proteasome pathway (UPP) degradation, thereby promoting sperm capacitation. These results provide new theoretical insights into the molecular mechanisms of sperm capacitation.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107794"},"PeriodicalIF":2.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective standardization of bovine frozen semen sperm motility analysis in a multi-laboratory setting

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-06 DOI: 10.1016/j.anireprosci.2025.107793

Leonardo F.C. Brito , Clara González-Marín, Pablo Ross

Sperm motility is the only functional parameter that is part of basic semen analysis, and its results have important implications for clinical practice and the production of semen doses for artificial insemination. However, substantial variations in sperm motility analysis within and across laboratories have been reported. The objective of the present study was to evaluate the precision and accuracy of sperm motility analysis in a controlled, multi-laboratory setting. Ten batches of conventional and sexed bovine frozen semen were produced, with three replicates of the same batch coded with different identification numbers. Sperm motility evaluations were performed using computer-assisted sperm analysis (CASA) systems by nine technicians across six laboratories, during three test periods conducted over one year. The overall intra-technician CV was 14.7 % for conventional semen and 13.9 % for sexed semen. Bland-Altman plots showed that the mean differences of individual test results from the overall mean were close to zero (-0.4–1.1 %), with most differences (61.5 %) ranging between ± 5 %. In conclusion, precise and accurate sperm motility results were obtained by qualified technicians when following standardized procedures and utilizing CASA systems.

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引用次数: 0

Protective effect of supplementation with water soluble β-carotene and α-tocopherol in boar sperm cooling-freezing extender, but not in the thawing extender

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-04 DOI: 10.1016/j.anireprosci.2025.107792

Adrián Martín-San Juan , Eduardo de Mercado , Helena Nieto-Cristóbal , Andrea Cabero , Miguel Ángel Silvestre , Jane M. Morrell , Manuel Álvarez-Rodríguez

Cryopreservation of sperm is a crucial tool for the long-term preservation of male genetic material, causing significant issues in motility, membrane, and acrosome integrity, among other parameters. Antioxidants have been used to cope with these detrimental effects. We tested 1. the toxicity of a wide range of water soluble β-carotene (β: 250–4000 µM) and α-tocopherol (α: 31–496 µM) concentrations on boar ejaculated sperm (n = 6) in parameters as motility, viability, acrosome reaction, apoptosis, oxidation, mitochondrial activation and membrane potential; 2. the effect of various β-carotene (250–1000 µM) and α-tocopherol (31–124 µM) concentrations added to the cooling-freezing or thawing extenders (n = 30) before (0 min) and after 90 min incubation (37 °C). Toxicity results showed a decrease in the proportion of live spermatozoa with non-reacted acrosome from 75.1 ± 3.3 % using β250/α31 to 60.1 ± 5.7 % and 59.3 ± 5.4 % in samples with β2000/α248 and β4000/α496 respectively (p < 0.05), suggesting a detrimental effect of the highest concentrations. Antioxidant supplementation in the cooling-freezing extender decreased the apoptotic and oxidized spermatozoa in β500/α62 and β1000/α124, relative to the control. In contrast, antioxidants addition to the thawing extender induced some detrimental effects in several sperm parameters analyzed. In conclusion, water-soluble β-carotene and α-tocopherol prevent acrosome reaction and oxidation during cooling-freezing on boar sperm. High concentrations of these antioxidants negatively impacted motility and mitochondrial function, suggesting cytotoxic effects and potential capacitation-like changes. The β1000/α124 showed protective effects during cryopreservation, but post-thawing supplementation may stimulate oxidative stress rather than prevent it.

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引用次数: 0

Selenium yeast alleviates diquat-induced oxidative stress and testicular damage in roosters 酵母硒可减轻diquat诱导的公鸡氧化应激和睾丸损伤。

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-01 DOI: 10.1016/j.anireprosci.2024.107760

Ningna Xiong , Sili Liu , Wei Hu , Yamei Liu , Xiang Ding , Deming Xu , Yiqiang Ling , Manxin Fang , Ben Liu , Lucheng Zheng , Wenya Zheng , Bingyan Wu

Diquat (DQ) is a pro-oxidant that generates free radicals in cells through redox reactions, leading to the induction of oxidative stress. During the processes of growth and reproduction, poultry are particularly vulnerable to oxidative stress. Selenium yeast (SeY) serves as an organic selenium source characterized by high activity and low toxicity, imparting antioxidant effects. The objective of this study was to assess the protective effects of SeY against DQ-induced oxidative stress in rooster testicles.The results demonstrated that SeY pretreatment mitigated DQ-induced oxidative damage in the testes. This mitigation encompassed the alleviation of inhibited spermatogenesis, reduced spermatogenic cell abundance, and the alleviation of decreased expression of genes StAR, P450scc, and 3β-HSD which related to testosterone synthesis. Specifically, SeY pretreatment counteracted DQ-induced oxidative stress by activating the Nrf2/HO-1 antioxidant signaling pathway, enhancing the activity of antioxidant enzymes such as catalase (CAT) and total superoxide dismutase (T-SOD), and reducing the concentration of malondialdehyde (MDA). Furthermore, SeY pretreatment attenuated DQ-induced spermatogonia apoptosis by modulating the expression of apoptosis-related genes and proteins, including Bax, Bcl-2, Caspase3, and NF-κB. Additionally, SeY restored the proliferative capacity of spermatogenic cells by promoting the expression of the proliferation-related protein Ki67. The aforementioned findings signify that SeY effectively safeguards the testes against DQ-induced damage through mechanisms involving the reduction of oxidative stress, inhibition of apoptosis, promotion of proliferation, and enhancing the expression of testosterone synthesis related genes. This study lays a solid theoretical foundation for future research aimed at safeguarding the reproductive health of male poultry exposed to agricultural pesticides.

敌草快（DQ）是一种促氧化剂，可通过氧化还原反应在细胞中产生自由基，从而诱发氧化应激。家禽在生长和繁殖过程中特别容易受到氧化应激的影响。硒酵母（SeY）是一种有机硒源，具有高活性、低毒性的特点，能起到抗氧化作用。本研究的目的是评估硒酵母对公鸡睾丸中 DQ 诱导的氧化应激的保护作用。结果表明，SeY 预处理减轻了 DQ 诱导的睾丸氧化损伤。这种减轻包括精子发生受抑制、生精细胞数量减少，以及与睾酮合成有关的基因 StAR、P450scc 和 3β-HSD 表达减少。具体而言，SeY通过激活Nrf2/HO-1抗氧化信号通路，提高过氧化氢酶（CAT）和总超氧化物歧化酶（T-SOD）等抗氧化酶的活性，降低丙二醛（MDA）的浓度，从而抵消DQ诱导的氧化应激。此外，SeY预处理通过调节凋亡相关基因和蛋白（包括Bax、Bcl-2、Caspase3和NF-κB）的表达，减轻了DQ诱导的精原细胞凋亡。此外，SeY 还能促进增殖相关蛋白 Ki67 的表达，从而恢复生精细胞的增殖能力。上述研究结果表明，SeY 通过降低氧化应激、抑制细胞凋亡、促进细胞增殖和提高睾酮合成相关基因的表达等机制，有效保护睾丸免受 DQ 引起的损伤。这项研究为今后旨在保护接触农药的雄性家禽生殖健康的研究奠定了坚实的理论基础。

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引用次数: 0

SUMO1 modification reduces oxidative stress and SUMO1ylated AKAP4 degradation affects frozen-thawed boar sperm quality SUMO1修饰降低氧化应激，SUMO1化的AKAP4降解影响冻融猪精子质量。

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-02-01 DOI: 10.1016/j.anireprosci.2024.107759

Yuyang Zhang, Xuan Chen, Lipeng Cao, Junzheng Zhang, Jie Wang, Zhiwei Yao, Kun Zhao, Yi Jin

Low-temperature injury affects normal physiological function and viability of boar sperm during cryopreservation. Small ubiquitin-like modifier (SUMO) modification of proteins after translation is related to the cell stress response but the relationship between SUMO modification and oxidative stress in freeze–thawed sperm remains unclear. A-kinase ankyrin 4 (AKAP4) and its precursor proAKAP4 are two main proteins in mammalian sperm. Although AKAP4 expression has been studied in many species, its expression in porcine sperm has not been described in detail. In this study, liquid chromatography-mass spectrometry was used to determine the differentially expressed SUMO-modified proteins in porcine sperm after freezen and thawed. The results identified 26 down-regulated SUMO-modified proteins, with AKAP4 identified as one of the target proteins of SUMO1 under sperm stress. In addition, the level of SUMO1 protein increased significantly (P < 0.001) and the level of AKAP4 protein decreased (P < 0.05) after freezing and oxidative stress treatment. Inhibition of SUMO1 modification of AKAP4 protein did not affect its degradation (P > 0.05), indicating that SUMO1 is not involved in the degradation of AKAP4. The inhibition of SUMO1 modification by sperm protein decreased sperm motility (P < 0.05), ATP content, and DNA integrity (P < 0.05). In summary, cryopreservation and oxidative stress can induce SUMO modification of porcine sperm proteins and the modification of sperm protein SUMO1 can help sperm resist oxidative stress; and its role in protecting sperm quality is not via regulating the degradation of AKAP4. These findings provide new insights into the mechanisms underlying SUMO1 modifications during sperm cryopreservation and oxidative stress.

低温损伤影响猪精子在低温保存过程中的正常生理功能和生存能力。蛋白质翻译后的小泛素样修饰物（Small ubiquitin-like modifier， SUMO）修饰与细胞应激反应有关，但SUMO修饰与冷冻解冻精子氧化应激的关系尚不清楚。a激酶锚蛋白4 （AKAP4）及其前体proAKAP4是哺乳动物精子中的两种主要蛋白。虽然AKAP4在许多物种中的表达已被研究，但其在猪精子中的表达尚未得到详细描述。本研究采用液相色谱-质谱联用技术测定猪精子冷冻和解冻后sumo修饰蛋白的差异表达。结果鉴定出26个下调的sumo修饰蛋白，其中AKAP4被鉴定为精子应激下SUMO1的靶蛋白之一。此外，SUMO1蛋白水平显著升高（P  0.05），表明SUMO1不参与AKAP4的降解。精子蛋白对SUMO1修饰的抑制降低了精子活力(P

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引用次数: 0