Animal Reproduction Science最新文献

Lycopene as a protective antioxidant in sperm preservation. 番茄红素在精子保存中的保护性抗氧化剂作用。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-09 DOI: 10.1016/j.anireprosci.2025.108075

Mohsen Shayestehyekta, Azita Faramarzi, Zahra Rashidi, Mojtaba Moradi

Sperm preservation is a pivotal technique in reproductive science, facilitating the long-term preservation and utilization of valuable genetic material, particularly in animal breeding programs and assisted reproductive technologies (ART). However, the freezing-thawing process imposes significant physiological stress on sperm cells, primarily due to oxidative damage. This oxidative stress disrupts critical cellular functions, leading to reduced sperm motility, viability, DNA integrity, and membrane stability, thereby compromising overall reproductive potential. Among various antioxidant strategies, lycopene, a potent carotenoid, has emerged as a promising candidate for mitigating cryoinjury. This review provides a comprehensive overview of lycopene's role in sperm cryopreservation and cold storage, emphasizing its effect on post-thaw sperm quality. Experimental evidence from animal studies indicates that lycopene supplementation effectively neutralizes reactive oxygen species, reduces lipid peroxidation, and preserves structural integrity by integrating into cell membranes. These protective effects contribute to enhancing sperm functionality post-thaw, potentially improving fertilization outcomes. Yet, this review critically highlights that the efficacy of lycopene is a double-edged sword: its effects are highly dose- and species-dependent, with excessive concentrations paradoxically impairing sperm performance. We conclude that a 'one-size-fits-all' approach is ineffective, and future investigations must move beyond simple supplementation to focus on optimizing species-specific formulations and validating in vitro benefits with in vivo fertility trials to fully harness lycopene's potential in reproductive applications.

精子保存是生殖科学的一项关键技术，有助于长期保存和利用有价值的遗传物质，特别是在动物育种计划和辅助生殖技术（ART）中。然而，冻融过程对精子细胞施加了显著的生理压力，主要是由于氧化损伤。这种氧化应激破坏了关键的细胞功能，导致精子活力、活力、DNA完整性和膜稳定性降低，从而损害了整体生殖潜力。在各种抗氧化策略中，番茄红素是一种有效的类胡萝卜素，已成为减轻低温损伤的有希望的候选者。本文综述了番茄红素在精子低温保存和冷藏中的作用，重点介绍了番茄红素对解冻后精子质量的影响。来自动物研究的实验证据表明，补充番茄红素可以有效地中和活性氧，减少脂质过氧化，并通过整合到细胞膜中来保持结构完整性。这些保护作用有助于提高解冻后精子的功能，潜在地改善受精结果。然而，这篇综述批判性地强调了番茄红素的功效是一把双刃剑：它的作用是高度剂量和物种依赖的，浓度过高反而会损害精子的表现。我们的结论是，“一刀切”的方法是无效的，未来的研究必须超越简单的补充，专注于优化特定物种的配方，并通过体内生育试验验证番茄红素的体外益处，以充分利用番茄红素在生殖应用中的潜力。

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引用次数: 0

Maturity determination and its potential application for fish captive breeding 成熟度测定及其在鱼类圈养养殖中的潜在应用

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-09 DOI: 10.1016/j.anireprosci.2025.108074

Darmawan Setia Budi , Agus Priyadi , Asep Permana , Ikhsan Khasani , Tamás Müller , Ahmad Shofy Mubarak , Imam Mustofa

Habitat degradation, overfishing, pollution, and climate change threaten many fish species, necessitating urgent conservation efforts. Captive breeding is essential for fish species recovery and genetic diversity preservation, with maturity determination being crucial for broodstock management, breeding success, and sustainable aquaculture practices. Standardized, species-specific protocols and non-invasive methods for accurately determining fish maturity are currently lacking. This review synthesizes current methodologies and practical applications of maturity determination in fish captive breeding, emphasizing how established knowledge can be applied to improve broodstock management and conservation. Traditional methods, like morphological and gonadal assessments, remain foundational, while histological and molecular techniques offer enhanced precision but are resource-intensive. Non-invasive imaging methods, including ultrasound, provide innovative alternatives for evaluating gonadal development. Behavioral cues, such as aggression, shoaling, and nesting, complement physiological assessments, enhancing accuracy. A multi-method approach is essential to address the diverse reproductive strategies of fish species. Advances in camera-based technologies and diagnostic kits for hormone detection offer novel, non-invasive, and scalable maturity assessments, reducing stress on fish. The integration of maturity determination with aquaculture facilitates broodstock selection, stock enhancement, and conservation efforts, promoting the long-term sustainability of aquatic ecosystems. This review highlights the need for standardized, species-specific protocols and technological advancements to support sustainable fish breeding. Future research should prioritize developing accessible tools and methodologies that integrate traditional and modern approaches, enhancing biodiversity conservation and promoting aquaculture growth.

栖息地退化、过度捕捞、污染和气候变化威胁着许多鱼类，迫切需要采取保护措施。圈养养殖对鱼类物种恢复和遗传多样性保护至关重要，而成熟度测定对亲鱼管理、繁殖成功和可持续水产养殖做法至关重要。目前还缺乏准确测定鱼类成熟度的标准化、特定物种的协议和非侵入性方法。本文综述了鱼类圈养繁殖中成熟度测定的现有方法和实际应用，强调了如何将已有的知识应用于改善亲鱼的管理和保护。传统的方法，如形态学和性腺评估，仍然是基础，而组织学和分子技术提供了更高的精度，但资源密集。非侵入性成像方法，包括超声，为评估性腺发育提供了创新的选择。行为线索，如攻击性、鱼群和筑巢，补充了生理评估，提高了准确性。多方法研究是解决鱼类繁殖策略多样性问题的关键。基于相机的技术和激素检测诊断试剂盒的进步提供了新颖、无创、可扩展的成熟度评估，减少了鱼类的压力。将成熟度测定与水产养殖相结合，有助于种鱼选择、种群增加和保护工作，促进水生生态系统的长期可持续性。本综述强调需要制定标准化的、特定物种的协议和技术进步，以支持可持续的鱼类养殖。未来的研究应优先开发易获取的工具和方法，将传统和现代方法结合起来，加强生物多样性保护，促进水产养殖增长。

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引用次数: 0

Impact of genetic selection for postweaning weight on the reproductive development of young Nellore bulls 断奶后体重遗传选择对内洛尔幼公牛生殖发育的影响。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-08 DOI: 10.1016/j.anireprosci.2025.108073

Marcelo Sant’Ana Borges , Letícia Padovani da Silva , Marina de Oliveira Silva , Mariana Karla Francolino da Silva , Victor Guidi Zardo , Laura Fernanda Sechirolli Silva , Ricardo Dutra Do Bem , André Maciel Crespilho , Maria Eugênia Zerlotti Mercadante , Fabio Morato Monteiro

This study aimed to evaluate the impact of genetic selection for increased postweaning weight on the reproductive development of young Nellore bulls. Seventy-six young Nellore bulls from two distinct selection lines in two experimental years were used: 23 young Nellore Control (NeC) bulls (14.1 ± 0.9 months old), and 53 young Nellore Selection (NeS) bulls (13.9 ± 0.8 months old). The animals were evaluated from 14 to 24 months of age at a mean interval of 58.4 ± 8.5 days. In each assessment, testicular biometry, B-mode testicular ultrasound, Doppler ultrasound of pampiniform plexus, and seminal evaluation were performed. Ten young bulls from each selection line underwent testicular fine-needle aspiration and were subsequently castrated at 18 months old for histological analysis of testicular tissue. Young bulls from the NeS line exhibited greater mean scrotal circumference (P < 0.0001), testicular volume (P < 0.0001), and end diastolic velocity (P < 0.0001) compared to the NeC line. The mean vascular resistance (P < 0.0001) and pulsatility (P < 0.0001) indices were greater in NeC animals than in NeS animals. Genetic selection of young Nellore bulls for increased postweaning weight resulted in greater scrotal circumference, testicular volume, and end diastolic velocity, indicating more pronounced testicular growth. No differences were observed in the seminal, cytological, or histological parameters between selection lines. These findings suggest that selection for increased postweaning weight gain did not compromise reproductive development, promoted an increase in biometric testicular parameters without negative effect on sperm quality or testicular tissue structure.

本研究旨在评估遗传选择对断奶后体重增加对内洛尔公牛生殖发育的影响。选取两个试验年的两个不同选择系的76头Nellore公牛：对照（NeC）公牛23头（14.1 ± 0.9月龄），NeS公牛53头（13.9 ± 0.8月龄）。实验时间为14 ~ 24月龄，平均间隔58.4 ± 8.5天。在每次评估中，进行睾丸生物测量、b超、多普勒睾丸丛超声和精液评估。每个选择系10头公牛进行睾丸细针穿刺，随后在18个月大时阉割睾丸组织进行组织学分析。NeS系幼牛的平均阴囊周长(P

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引用次数: 0

A pilot study on anogenital distance in non-pregnant and pregnant Merino ewes and ewe hoggets measured by calipers and image analysis 用卡尺和图像分析方法测定美利奴母羊和母羊未怀孕和怀孕时肛门生殖器距离的初步研究

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-08 DOI: 10.1016/j.anireprosci.2025.108072

Tianna R. Boyce, Lillian P. Carlyon, Kiro R. Petrovski, Leesa-Joy Dunstan, Bruno RC Alves, Gebremeskel Mamu Werid, Mariana Caetano

Reproductive traits are fundamental phenotypic traits selected to achieve optimal production efficiency. The aim of this study was to evaluate the use of four measurements of anogenital distances (AGD; in mm) and compare them with ewe pregnancy status, measured via pregnancy scanning, using data collected from 80 Merino ewes and 80 ewe hoggets. Digital calipers were used to measure AGDs (AC – anus to clitoris, AVV – anus to ventral vulva commissure, AF – anus to fourchette and VV – vulva length) in each sheep twice by three assessors. High-resolution images were taken for each sheep to conduct further measurements of AGD using an image analysis software. The measurements conducted with the image analysis were correlated with the pregnancy status of ewe hoggets for AF (P < 0.002), VV (P < 0.001) and AVV (P < 0.002), but no association was found for AC (P > 0.05). The measurements performed with calipers by three assessors were more variable compared to image analysis, resulting in lower correlations between the first and second measurements taken on the same animal. The relationship found between three AGD measurements (AF, AVV and VV), and pregnancy status in the ewe hoggets, demonstrates the potential to use this trait as an inexpensive method to estimate pregnancy status. The association with pregnancy status, especially in ewe hoggets, could be easily adopted by producers.

生殖性状是为实现最佳生产效率而选择的基本表型性状。本研究的目的是评估肛门生殖器距离（AGD，以毫米为单位）的四种测量方法的使用，并将其与母羊妊娠状态进行比较，母羊妊娠状态通过妊娠扫描测量，使用收集自80只美利奴母羊和80只母羊的数据。采用数字卡尺对每只羊的AGDs （AC -肛门到阴蒂，AVV -肛门到外阴腹侧连接，AF -肛门到阴道，VV -外阴长度）进行3次测量。为每只羊拍摄高分辨率图像，使用图像分析软件进一步测量AGD。通过图像分析进行的测量结果与母羊妊娠状态的AF （P <； 0.002）、VV （P <； 0.001）和AVV （P <； 0.002）相关，但与AC （P <； 0.05）无关。与图像分析相比，由三名评估人员用卡尺进行的测量更具可变性，导致同一动物的第一次和第二次测量之间的相关性较低。三种AGD测量值（AF、AVV和VV）与母羊妊娠状态之间的关系表明，利用这一特征作为一种廉价的方法来估计妊娠状态的潜力。与妊娠状态的关联，特别是母羊，很容易被生产者采用。

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引用次数: 0

Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.) 伊比利亚生态型和欧洲生态型的精子功能和形态差异

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-08 DOI: 10.1016/j.anireprosci.2025.108071

J. Gómez-Delgado , B. Martínez-Madrid , A. Toledano-Díaz , C. Castaño , A. Gómez-Crespo , G. de Pedro Aguilar , D. Cubero , A. Kowalczyk , E. Łukaszewicz , J. Santiago-Moreno

The capercaillie (Tetrao urogallus L.) is a threatened forest bird with distinct Iberian and European ecotypes. This study examines the functional and morphometric differences between the sperm of these ecotypes. In a first experiment, semen volume, sperm concentration, motility, viability, and DNA integrity were assessed in 'Iberian' and 'European' capercaillies, both maintained at breeding centres in Spain. In a second experiment, morphometric traits were measured in Iberian capercaillies maintained at a breeding centre in Spain, and in European capercaillies also maintained at breeding centres in Spain (EmS birds) and in Poland (EmP birds). In the first experiment, the European males produced larger ejaculate volumes (39.80 ± 3.56 µL) than the Iberian males (29.68 ± 4.64 µL). However, the Iberian males returned significantly higher sperm concentrations (501.99 ± 83.90 × 10⁶ spz/mL vs. 77.66 ± 26.09 × 10⁶ spz/mL). In the second experiment, the origin of the birds also affected (P < 0.001) sperm head dimensions. These were smaller in the EmP birds compared to the EmS birds (P < 0.01), and compared to Iberian males (always maintained in Spain) (P < 0.001). Within each of these groups, three sperm subpopulations were identified according to head dimensions, with differences (P < 0.001) between these groups in terms of the proportion of each subpopulation. This is the first comprehensive study of sperm morphometric characteristics in these capercaillie ecotypes. These results may provide critical insights into the reproductive and evolutionary strategies of capercaillies and contribute to improving the success of reproductive technologies across different ecotypes and populations.

capercaillie （Tetrao urogallus L.）是一种濒危的森林鸟类，具有独特的伊比利亚和欧洲生态型。本研究考察了这些生态型精子之间的功能和形态差异。在第一个实验中，对“伊比利亚”和“欧洲”的精子数量、精子浓度、活力、生存能力和DNA完整性进行了评估，这两种精子都保存在西班牙的繁殖中心。在第二个实验中，对饲养在西班牙繁殖中心的伊比利亚和饲养在西班牙（EmS鸟类）和波兰（EmP鸟类）繁殖中心的欧洲的capercailes进行了形态计量学特征测量。在第一个实验中，欧洲雄性的射精量（39.80 ± 3.56 µL）大于伊比利亚雄性（29.68 ± 4.64 µL）。然而，伊比利亚雄鼠返回的精子浓度明显更高（501.99 ± 83.90 × 10⁶spz/mL vs. 77.66 ± 26.09 × 10⁶spz/mL）。在第二个实验中，鸟类的起源也会影响精子头部尺寸（P <； 0.001）。与EmP鸟类相比，EmP鸟类的这些较小（P <； 0.01），与伊比利亚雄性（一直在西班牙饲养）相比（P <； 0.001）。在这些组中，根据头部尺寸确定了三个精子亚群，这些组之间在每个亚群的比例方面存在差异（P <； 0.001）。这是第一次对这些花椒生态型的精子形态特征进行全面研究。这些结果可能为研究长尾虫的繁殖和进化策略提供重要的见解，并有助于提高不同生态型和种群的繁殖技术的成功率。

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引用次数: 0

Pharmacological activation of Wnt/β-catenin signaling is essential for oocyte developmental competence and enhances in vitro embryo production in water buffalo Wnt/β-catenin信号的药理激活对水牛卵母细胞发育能力和体外胚胎生成至关重要

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-04 DOI: 10.1016/j.anireprosci.2025.108070

Xiaoya Ma , Haiying Zheng , Anqin Duan , Yingxue Xie , Jinchen Zhou , Jianghua Shang , Chunyan Yang

The Wnt/β-catenin signaling pathway is integral to follicular development, yet its precise role in buffalo folliculogenesis remains poorly defined. This study aimed to elucidate its involvement in follicular selection and underlying regulatory mechanisms. Buffalo follicles were classified as dominant or atretic based on the follicular fluid estradiol-to-progesterone (E₂: P₄) ratio. Quantitative PCR revealed significantly elevated expression of Wnt/β-catenin pathway components in the granulosa cells of dominant follicles. Functional experiments demonstrated that pharmacological inhibition of Wnt/β-catenin signaling markedly suppressed both FSH-induced estradiol (E₂) production and oocyte maturation. Conversely, Wnt/β-catenin pathway activation enhanced oocyte maturation, cleavage, and blastocyst formation rates. Further investigation identified AKT/GSK3β/β-catenin cascade as a key mediator of Wnt's positive regulatory role in FSH-supported steroidogenesis. These findings clarify the pivotal role of Wnt/β-catenin signaling in buffalo follicular selection and provide a foundational strategy for enhancing the efficiency of in vitro embryo production (IVP) in buffalo breeding programs.

Wnt/β-catenin信号通路是卵泡发育不可或缺的一部分，但其在水牛卵泡发生中的确切作用仍不清楚。本研究旨在阐明其参与卵泡选择及其潜在的调控机制。根据卵泡液雌二醇与黄体酮（E2: P4）的比值将水牛卵泡分为显性卵泡和闭锁卵泡。定量PCR结果显示，优势卵泡颗粒细胞中Wnt/β-catenin通路组分的表达显著升高。功能实验表明，药理抑制Wnt/β-catenin信号传导可显著抑制fsh诱导的雌二醇（E2）产生和卵母细胞成熟。相反，Wnt/β-catenin通路激活可提高卵母细胞成熟、卵裂和囊胚形成率。进一步的研究发现AKT/GSK3β/β-catenin级联是Wnt在fsh支持的类固醇生成中积极调节作用的关键介质。这些发现阐明了Wnt/β-catenin信号在水牛卵泡选择中的关键作用，并为提高水牛体外胚胎生产（IVP）的效率提供了基础策略。

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引用次数: 0

Influence of sexual maturity on sperm quality in Duroc boars 性成熟对杜洛克公猪精子质量的影响。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-02 DOI: 10.1016/j.anireprosci.2025.108053

Soudabeh Rouzbehani , Asmita Shrestha , Nazia Haque , Syeda Afsana Nushrat , Teklu Tewoldebrhan Zeremichael , Anna Nordborg , Ann Helen Gaustad , Anne Hege Alm-Kristiansen

The objective of this study was to evaluate sperm quality of Duroc boars during sexual maturation and examine whether these parameters were related to metabolites in semen. Ejaculates were collected longitudinally from 28 Duroc boars at approximately 7 months of age (Age 1), 6 weeks later (Age 2) and 12 weeks later (Age 3). Motility characteristics, acrosome integrity, and viability were assessed on collection day and after five days storage at 18°C using computer-assisted sperm analysis and flow cytometry, while DNA fragmentation was measured on day five. Amino acid and amine concentrations in semen were measured by liquid chromatography-mass spectrometry and correlated with sperm quality parameters. Motility, rapid progressive motility, and acrosome intact live sperm increased significantly (adj P < 0.05) with boar maturation. Mature boars (Age 2 and Age 3) maintained superior sperm quality compared to younger boars after storage. The DNA fragmentation index was low in all samples but declined significantly from 1 % at Age 1 to 0.64 % at Age 3 (adj P < 0.05). Correlation analysis showed significant relationships between sperm quality and specific metabolites. Cystine, glutamate, aspartate, choline and taurine were inversely correlated with progressive and rapid progressive motility, while showing positive correlation with rapid non-progressive motility. These findings demonstrate that sperm quality continues improving beyond initial reproductive ability, especially between Age 1 and Age 3, with improvements in motility, viability, chromatin stability, and storage resilience. The observed relationships between metabolites and sperm quality parameters provide insights into biochemical mechanisms underlying sperm functionality during sexual maturation.

本研究的目的是评价杜洛克公猪性成熟期间的精子质量，并探讨这些参数是否与精液中的代谢物有关。28头杜洛克公猪分别在大约7月龄（1岁）、6周（2岁）和12周（3岁）时纵向采集射精。在采集当天和18°C保存5天后，使用计算机辅助精子分析和流式细胞术评估运动特性、顶体完整性和活力，并在第5天测量DNA片段。采用液相色谱-质谱法测定精液中氨基酸和胺的浓度，并与精子质量参数进行相关性分析。活力、快速进行性活力和顶体完整活精子显著增加（P < 0.05）

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引用次数: 0

Histomorphometric characterization of the endometrium in mules (Equus mulus): An approach to endometritis/endometrosis 骡子子宫内膜的组织形态学特征：子宫内膜炎/子宫内膜异位症的一种方法。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-02 DOI: 10.1016/j.anireprosci.2025.108056

Miguel A. Gutiérrez-Reinoso , Pedro M. Aponte , Manuel García-Herreros , Katlhen G. García-Bravo , Daniela Rojas , Yat Sen Wong , Fernando Saravia , Fidel Ovidio Castro , Jaime Catalán , Jordi Miró

Mules (Equus mulus), as sterile hybrids between mares and donkeys, present a unique uterine morphology and physiology that remains poorly characterized. This study provides the first histomorphometric evaluation and transcriptional profiling of the endometrium in adult mules. To achieve this, endometrial biopsies were analysed using quantitative stereology, histological classification (Kenney–Doig system), and qPCR of key immune-related and fibrotic genes. Histometric analyses revealed that the stratum spongiosum was the predominant component of the mule endometrium (mean volume density: 84.7 %), whereas the epithelium and compact layers accounted for smaller proportions. This distribution, more pronounced than what is typically reported in mares, suggests enhanced stromal expansion or edema. Endometrial glands were abundant, and the overall structural profile exhibited an intermediate phenotype with a clear asinine bias. Most samples were classified as grades I, IIA, or IIB, with no specimens reaching grade III. Mild to moderate stromal remodeling, preserved tissue architecture, and inflammatory signatures –characterized by increased relative abundance of IL1B, IL6, and TNFα transcripts– were observed in higher grades and were consistent with early stages of endometrial degeneration. Although these features indicate early degenerative changes, the hybrid reproductive physiology of mules, including limited hormonal cyclity, may contribute to reduced progression towards fibrosis. This integrated histological–molecular assessment reinforces the diagnostic value of the Kenney–Doig system in mules and supports the adaptation of a refined IIC grade to better capture hybrid-specific features. Overall, the mule endometrium displays distinct yet comparable traits relative to that of horses and donkeys, highlighting its potential as a comparative model for equine reproductive research. These findings provide a foundation for improved clinical decision-making and future studies on hybrid fertility and uterine health.

骡子（Equus mulus）是母马和驴之间的不育杂种，具有独特的子宫形态和生理特征。这项研究首次提供了成年骡子子宫内膜的组织形态学评估和转录谱分析。为了实现这一点，使用定量立体学、组织学分类（kennedy - doig系统）和关键免疫相关基因和纤维化基因的qPCR对子宫内膜活检进行分析。组织计量学分析显示，海绵层是骡子子宫内膜的主要成分（平均体积密度：84.7 %），而上皮和致密层占较小的比例。这种分布比母马的典型报告更为明显，表明间质扩张或水肿增强。子宫内膜腺丰富，整体结构表现出明显的asinine偏倚的中间表型。大多数标本被划分为I、IIA或IIB级，没有标本达到III级。轻度至中度间质重塑、保留的组织结构和炎症特征——以IL1B、IL6和TNFα转录物相对丰度增加为特征——在更高级别的子宫内膜变性中被观察到，并且与早期阶段一致。尽管这些特征表明早期退行性改变，但骡子的杂交生殖生理，包括有限的激素周期，可能有助于减少纤维化的进展。这种综合的组织学-分子评估强化了kennedy - doig系统在骡子中的诊断价值，并支持改进的IIC分级，以更好地捕捉杂交特异性特征。总的来说，与马和驴相比，骡子的子宫内膜显示出不同但可比较的特征，突出了其作为马生殖研究的比较模型的潜力。这些发现为改进临床决策和进一步研究杂交生育和子宫健康提供了基础。

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引用次数: 0

Effect of post-mating TAK-683 (Kisspeptin analog) treatment on luteal morphology and function in suckling goats with lactational anestrus 交配后处理TAK-683 （Kisspeptin类似物）对哺乳期停发山羊黄体形态和功能的影响。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-02 DOI: 10.1016/j.anireprosci.2025.108052

Gökhan Uyanık , Murat Yüksel , Murat Abay , Ahmet Gözer , Ufuk Kaya , Filiz Kara , Ebru Arslanhan , Ishak Gökçek

This study aimed to evaluate the effects of TAK-683 administered to suckling goats in lactational anestrus on the 4th day (D4) after mating on luteal function, serum progesterone (P₄) levels, and reproductive performance. Forty-five Aleppo goats were treated with intravaginal sponges containing medroxyprogesterone acetate for 7 days and were injected intramuscularly with 500 IU eCG and 75 µg d-cloprostenol on the day of sponge removal. Thirty-seven goats showed estrus and were mated with fertile bucks (D0). The mated goats were randomly divided into groups to receive subcutaneous administration of saline (Control, n = 12), 5 μg TAK-683 (TAK-5, n = 12) or 25 μg TAK-683 (TAK-25, n = 13) on D4. Transrectal ovarian ultrasonography and blood sampling were performed on D4, D7, D14, D17, and D21, and pregnancy was diagnosed on D35. Regardless of dose, TAK-683 treatment induced the formation of accessory corpora lutea (aCL) and significantly increased original CL (oCL) diameter, luteal area (LA), and Doppler area (DA) compared with controls (P < 0.001). Total LA and DA were greater (P < 0.001) in TAK-5 and TAK-25, although DA/LA ratio did not differ (P > 0.05). Serum P₄ levels were significantly higher in TAK-25 from D7 to D21 (P < 0.001), while TAK-5 showed a delayed increase from D17. Luteal morphological parameters were strongly and positively correlated with circulating P₄ levels, confirming the luteotropic effect of TAK-683. TAK-683 treatment had no significant effect on fertility parameters (P < 0.05). In conclusion, although TAK-683 treatment enhanced luteal function and increased circulating P₄ concentrations, it failed to improve fertility outcomes in suckling goats under lactational anestrus conditions.

本研究旨在评价TAK-683在配种后第4天（D4）给药对哺乳山羊黄体功能、血清孕酮（硫酸铵）水平和繁殖性能的影响。选取45只阿勒颇山羊，用含醋酸甲孕酮的阴道内海绵治疗7 d，取海绵当日肌内注射500 IU eCG和75 µg d-氯前列醇。37只山羊出现发情，并与可育雄鹿交配（D0）。将配对山羊随机分为各组，分别于D4日皮下注射生理盐水（对照组，n = 12）、5 μg TAK-683 （TAK-5, n = 12）或25 μg TAK-683 （TAK-25, n = 13）。于D4、D7、D14、D17、D21行经直肠卵巢超声及采血，D35诊断妊娠。无论剂量如何，TAK-683处理均诱导副黄体（aCL）的形成，与对照组相比，原始CL （oCL）直径、黄体面积（LA）和多普勒面积（DA）显著增加（P  0.05）。从D7到D21， TAK-25患者血清中硫酸磷水平显著升高(P

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引用次数: 0

A new urinary biomarker reflecting the progesterone excretion pattern identified through serum steroid and urinary progesterone metabolite analysis during pro-oestrus and oestrus in female dogs (Canis familiaris) 一种新的反映孕酮排泄模式的尿液生物标志物，通过对母狗（犬）发情前和发情期间血清类固醇和尿孕酮代谢物的分析确定。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-02 DOI: 10.1016/j.anireprosci.2025.108054

Tia M. Pennanen , Merja R. Häkkinen , Risto O. Juvonen , Jouko J. Vepsäläinen , Seppo Auriola , Sari M. Viitala

In female dogs, the optimal breeding time is estimated by an increase in the serum progesterone hormone concentration. The method is not only invasive and unpleasant to dogs but also laborious and requires professional laboratory expertise. The main aim of this study was to explore potential urinary progesterone metabolites for non-invasive oestrus monitoring. Additionally, thirteen steroid hormones, such as sex steroids and corticosteroids, were determined in serum samples from nine female dogs via multiple reaction monitoring liquid chromatographymass spectrometry (LCMS). In addition, various urinary steroid metabolites from 22 dogs were analysed via nontargeted liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis. The serum oestrone, oestradiol, and progesterone concentration profiles were aligned with the existing literature. When the 17β-oestradiol secretion peak (49–83 pg/mL) during pro-oestrus was considered as Day 0, 17α-hydroxyprogesterone, testosterone, and androstenedione concentrations peaked on Days 0–3 (P-values 0.004, 0.015 and 0.007, respectively). Results of corticosteroids unveiled novel insights into steroid secretion during the breeding season of dogs. Parallel urine samples revealed that progesterone was excreted into the urine as the unidentified pregnanetriol glucuronide “PtGX” instead of the common metabolite pregnanediol-3-glucuronide (PdG) among mammals. The excretion profiles of pregnanetriol glucuronide support its usability as a biomarker in non-invasive oestrus monitoring in female dogs. The findings presented herein lay the foundation for a more animal-friendly and streamlined approach to monitoring the oestrus cycle of dogs, with substantial implications for dog breeding and advancing animal welfare practices.

在母狗中，通过增加血清黄体酮激素浓度来估计最佳繁殖时间。这种方法不仅有侵入性，而且对狗狗来说很不舒服，而且很费力，需要专业的实验室知识。本研究的主要目的是探索用于无创发情监测的潜在尿孕酮代谢物。此外，通过多重反应监测液相色谱-质谱法（LCMS）测定了9只母狗血清样本中的13种类固醇激素，如性类固醇和皮质类固醇。此外，通过非靶向液相色谱-高分辨率质谱（LC-HRMS）分析了22只狗的各种尿液类固醇代谢物。血清雌激素、雌二醇和黄体酮浓度谱与现有文献一致。将17β-雌二醇分泌高峰（49 ~ 83 pg/mL）视为发情期第0天，17α-羟孕酮、睾酮和雄烯二酮浓度在第0 ~ 3天达到峰值（p值分别为0.004、0.015和0.007）。皮质类固醇的结果揭示了在狗的繁殖季节类固醇分泌的新见解。平行尿液样本显示，在哺乳动物中，孕酮以未识别的妊娠三醇-葡糖苷“PtGX”的形式排入尿液，而不是常见的代谢物妊娠二醇-3-葡糖苷（PdG）。孕三醇葡萄糖醛酸酯的排泄谱支持其作为雌性犬无创发情监测生物标志物的可用性。本文提出的研究结果为采用一种更有利于动物的、简化的方法来监测狗的发情周期奠定了基础，对狗的繁殖和推进动物福利实践具有重大意义。

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引用次数: 0